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Citation for Study 19035

Citation title: "Cryphonectriaceae associated with rust infected Syzygium jambos in Hawaii".

Study name: "Cryphonectriaceae associated with rust infected Syzygium jambos in Hawaii".

This study is part of submission 19035 (Status: Published).

Citation

Nkuekam G.K., Roux J., Wingfield M.J., & Van der merwe N. 2016. Cryphonectriaceae associated with rust infected Syzygium jambos in Hawaii. Mycologia, .

Authors

• Nkuekam G.K. (submitter) 0027824354655
• Roux J.
• Wingfield M.J.
• Van der merwe N.

Abstract

Syzygium jambos trees infected by the Myrtle rust pathogen, Puccinia psidii, on the Hawaiian Islands commonly revealed species in the Cryphonectriaceae (Diaporthales). These fungi include at least nineteen genera of ascomycetes including important tree pathogens. In this study, S. jambos and other trees in the Myrtales were examined on three Hawaiian Islands for the presence of Cryphonectriaceae. Bark samples with fruiting bodies were collected from infected trees and fungi were isolated directly from these structures. Pure cultures were produced and the fungi were identified using both morphological characteristics and DNA sequence data for the internal transcribed spacer (ITS) regions, part of the β-tubulin (BT) and the transcription elongation factor-1α (TEF) gene regions. Representative isolates of the species collected were used in artificial inoculation studies to consider their pathogenicity to S. jambos. Five species of Cryphonectriaceae, residing in three genera were identified from samples originating from three tree genera in the Myrtaceae. These included Chrysoporthe deuterocubensis, Celoporthe guangdongensis, Microthia havanensis and two previously unknown taxa described here as Ce. hawaiiensis sp. nov. and Ce. hauoliensis sp. nov. Celoporthe guangdongensis, Ce. hawaiiensis and Chr. deuterocubensis produced lesions on young S. jambos trees in inoculation trials, suggesting that, together with P. psidii, they may contribute to the death of trees. Microsatellite markers were subsequently used to consider the diversity of Chr. deuterocubensis on the islands and thus to gain insights into its possible origin in Hawaii. Isolates of this important pathogen of the Myrtaceae, and particularly Eucalyptus, were found to be clonal. This provides evidence that Chr. deuterocubensis was introduced to the Hawaiian Islands as a single introduction from a currently unknown source.

Keywords

fungi, genetic diversity, Myrtales, pathogens, pathogen introductions

External links

About this resource

• Canonical resource URI: http://purl.org/phylo/treebase/phylows/study/TB2:S19035
• Other versions: Nexus NeXML
• Show BibTeX reference

  		@ARTICLE{TreeBASE2Ref25682,
  	 author = {Gilbert Kamgan Nkuekam and Jolanda  Roux and Michael J. Wingfield and Nicolaas A  Van der Merwe},
  	 title = {Cryphonectriaceae associated with rust infected Syzygium jambos in Hawaii},
  	 year = {2016},
  	 keywords = {fungi, genetic diversity, Myrtales, pathogens, pathogen introductions},
  	 doi = {},
  	 url = {http://},
  	 pmid = {},
  	 journal = {Mycologia},
  	 volume = {},
  	 number = {},
  	 pages = {},
  	 abstract = {Syzygium jambos trees infected by the Myrtle rust pathogen, Puccinia psidii, on the Hawaiian Islands commonly revealed species in the Cryphonectriaceae (Diaporthales). These fungi include at least nineteen genera of ascomycetes including important tree pathogens. In this study, S. jambos and other trees in the Myrtales were examined on three Hawaiian Islands for the presence of Cryphonectriaceae. Bark samples with fruiting bodies were collected from infected trees and fungi were isolated directly from these structures. Pure cultures were produced and the fungi were identified using both morphological characteristics and DNA sequence data for the internal transcribed spacer (ITS) regions, part of the β-tubulin (BT) and the transcription elongation factor-1α (TEF) gene regions. Representative isolates of the species collected were used in artificial inoculation studies to consider their pathogenicity to S. jambos. Five species of Cryphonectriaceae, residing in three genera were identified from samples originating from three tree genera in the Myrtaceae. These included Chrysoporthe deuterocubensis, Celoporthe guangdongensis, Microthia havanensis and two previously unknown taxa described here as Ce. hawaiiensis sp. nov. and Ce. hauoliensis sp. nov. Celoporthe guangdongensis, Ce. hawaiiensis and Chr. deuterocubensis produced lesions on young S. jambos trees in inoculation trials, suggesting that, together with P. psidii, they may contribute to the death of trees. Microsatellite markers were subsequently used to consider the diversity of Chr. deuterocubensis on the islands and thus to gain insights into its possible origin in Hawaii. Isolates of this important pathogen of the Myrtaceae, and particularly Eucalyptus, were found to be clonal. This provides evidence that Chr. deuterocubensis was introduced to the Hawaiian Islands as a single introduction from a currently unknown source.}
  }
  		

• Show RIS reference

  		TY  - JOUR
  ID  - 25682
  AU  - Nkuekam,Gilbert Kamgan
  AU  - Roux,Jolanda 
  AU  - Wingfield,Michael J.
  AU  - Van der Merwe,Nicolaas A 
  T1  - Cryphonectriaceae associated with rust infected Syzygium jambos in Hawaii
  PY  - 2016
  KW  - fungi
  KW  - genetic diversity
  KW  - Myrtales
  KW  - pathogens
  KW  - pathogen introductions
  UR  - http://dx.doi.org/
  N2  - Syzygium jambos trees infected by the Myrtle rust pathogen, Puccinia psidii, on the Hawaiian Islands commonly revealed species in the Cryphonectriaceae (Diaporthales). These fungi include at least nineteen genera of ascomycetes including important tree pathogens. In this study, S. jambos and other trees in the Myrtales were examined on three Hawaiian Islands for the presence of Cryphonectriaceae. Bark samples with fruiting bodies were collected from infected trees and fungi were isolated directly from these structures. Pure cultures were produced and the fungi were identified using both morphological characteristics and DNA sequence data for the internal transcribed spacer (ITS) regions, part of the β-tubulin (BT) and the transcription elongation factor-1α (TEF) gene regions. Representative isolates of the species collected were used in artificial inoculation studies to consider their pathogenicity to S. jambos. Five species of Cryphonectriaceae, residing in three genera were identified from samples originating from three tree genera in the Myrtaceae. These included Chrysoporthe deuterocubensis, Celoporthe guangdongensis, Microthia havanensis and two previously unknown taxa described here as Ce. hawaiiensis sp. nov. and Ce. hauoliensis sp. nov. Celoporthe guangdongensis, Ce. hawaiiensis and Chr. deuterocubensis produced lesions on young S. jambos trees in inoculation trials, suggesting that, together with P. psidii, they may contribute to the death of trees. Microsatellite markers were subsequently used to consider the diversity of Chr. deuterocubensis on the islands and thus to gain insights into its possible origin in Hawaii. Isolates of this important pathogen of the Myrtaceae, and particularly Eucalyptus, were found to be clonal. This provides evidence that Chr. deuterocubensis was introduced to the Hawaiian Islands as a single introduction from a currently unknown source.
  L3  - 
  JF  - Mycologia
  VL  - 
  IS  - 
  ER  -