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Citation for Study 23166

Citation title: "Molecular systematics of two sister clades, the Fusarium concolor and F. babinda species complexes, and the discovery of a novel microcycle macroconidium-producing species from South Africa ".

Study name: "Molecular systematics of two sister clades, the Fusarium concolor and F. babinda species complexes, and the discovery of a novel microcycle macroconidium-producing species from South Africa ".

This study is part of submission 23166 (Status: Published).

Citation

Jacobs-venter A., Laraba I., Geiser D., Busman M., Vaughan M.M., Proctor R.H., Mccormick S.P., & O'donnell K. 2018. Molecular systematics of two sister clades, the Fusarium concolor and F. babinda species complexes, and the discovery of a novel microcycle macroconidium-producing species from South Africa. Mycologia, .

Authors

• Jacobs-venter A.
• Laraba I.
• Geiser D.
• Busman M.
• Vaughan M.M.
• Proctor R.H.
• Mccormick S.P.
• O'donnell K. 309-681-6383

Abstract

Multilocus DNA sequence data were used to investigate species identity and diversity in two sister clades, the Fusarium concolor (FCOSC) and F. babinda species complexes. The molecular phylogenetic results support the recognition of a novel Fusarium species within the FCOSC, which is described and illustrated here as F. austroafricanum sp. nov. It was isolated as an endophyte of kikuyu grass associated with a putative mycotoxicosis of cattle and from plant debris in soil in South Africa. Molecular phylogenetic analyses indicated it represents a genealogically exclusive lineage that is sister to F. concolor. Of the 109 isolates analyzed, only four were received correctly identified to species and these included 1/46 F. concolor, 1/31 F. babinda and 2/3 F. anguioides. The majority of the F. concolor and F. babinda isolates were received as F. polyphialidicum, which is a heterotypic synonym of the former species. Previously documented from South America, Africa, Europe and Australia, our data show F. concolor is also present in North America. The present study expands the known distribution of F. babinda in Australia to Asia, Europe and North America. Fusarium austroafricanum is most similar morphologically to F. concolor and F. babinda but differs from the latter two species in producing: (i) much longer macroconidia in which the apical cell is blunt to slightly papillate and the basal cell is only slightly notched, (ii) macroconidia via microcycle conidiation on water agar, and (iii) head blight disease symptoms in inoculated spikelets of the susceptible wheat cultivar Norm. BLASTn searches of the whole genome sequence of F. austroafricanum NRRL 53441 were conducted to predict mycotoxin potential, using genes known to be essential for the synthesis of several mycotoxins and biologically active metabolites. Based on the presence of intact gene clusters that confer the ability to synthesize mycotoxins and pigments, we analyzed cracked corn kernel cultures of F. austroafricanum via liquid chromatography-mass spectrometry (LC-MS) but failed to detect these metabolites in vitro.

Keywords

Fusarium austroafricanum, Fusarium babinda, Fusarium polyphialidicum, gene genealogies, morphology, mycotoxins, pathogenicity, phylogenetic species recognition, 1 new taxon

External links

About this resource

• Canonical resource URI: http://purl.org/phylo/treebase/phylows/study/TB2:S23166
• Other versions: Nexus NeXML
• Show BibTeX reference

  		@ARTICLE{TreeBASE2Ref28735,
  	 author = {Adriaana  Jacobs-Venter and Imane  Laraba and David M.  Geiser and Mark  Busman and Martha M. Vaughan and Robert H. Proctor and Susan P. McCormick and Kerry  O'Donnell},
  	 title = {Molecular systematics of two sister clades, the Fusarium concolor and F. babinda species complexes, and the discovery of a novel microcycle macroconidium-producing species from South Africa },
  	 year = {2018},
  	 keywords = {Fusarium austroafricanum, Fusarium babinda, Fusarium polyphialidicum, gene genealogies, morphology, mycotoxins, pathogenicity, phylogenetic species recognition, 1 new taxon},
  	 doi = {},
  	 url = {http://},
  	 pmid = {},
  	 journal = {Mycologia},
  	 volume = {},
  	 number = {},
  	 pages = {},
  	 abstract = {Multilocus DNA sequence data were used to investigate species identity and diversity in two sister clades, the Fusarium concolor (FCOSC) and F. babinda species complexes.  The molecular phylogenetic results support the recognition of a novel Fusarium species within the FCOSC, which is described and illustrated here as F. austroafricanum sp. nov.   It was isolated as an endophyte of kikuyu grass associated with a putative mycotoxicosis of cattle and from plant debris in soil in South Africa.  Molecular phylogenetic analyses indicated it represents a genealogically exclusive lineage that is sister to F. concolor.  Of the 109 isolates analyzed, only four were received correctly identified to species and these included 1/46 F. concolor, 1/31 F. babinda and 2/3 F. anguioides.  The majority of the F. concolor and F. babinda isolates were received as F. polyphialidicum, which is a heterotypic synonym of the former species.  Previously documented from South America, Africa, Europe and Australia, our data show F. concolor is also present in North America.  The present study expands the known distribution of F. babinda in Australia to Asia, Europe and North America.  Fusarium austroafricanum is most similar morphologically to F. concolor and F. babinda but differs from the latter two species in producing: (i) much longer macroconidia in which the apical cell is blunt to slightly papillate and the basal cell is only slightly notched, (ii) macroconidia via microcycle conidiation on water agar, and (iii) head blight disease symptoms in inoculated spikelets of the susceptible wheat cultivar Norm.  BLASTn searches of the whole genome sequence of F. austroafricanum NRRL 53441 were conducted to predict mycotoxin potential, using genes known to be essential for the synthesis of several mycotoxins and biologically active metabolites.  Based on the presence of intact gene clusters that confer the ability to synthesize mycotoxins and pigments, we analyzed cracked corn kernel cultures of F. austroafricanum via liquid chromatography-mass spectrometry (LC-MS) but failed to detect these metabolites in vitro.  }
  }
  		

• Show RIS reference

  		TY  - JOUR
  ID  - 28735
  AU  - Jacobs-Venter,Adriaana 
  AU  - Laraba,Imane 
  AU  - Geiser,David M. 
  AU  - Busman,Mark 
  AU  - Vaughan,Martha M.
  AU  - Proctor,Robert H.
  AU  - McCormick,Susan P.
  AU  - O'Donnell,Kerry 
  T1  - Molecular systematics of two sister clades, the Fusarium concolor and F. babinda species complexes, and the discovery of a novel microcycle macroconidium-producing species from South Africa 
  PY  - 2018
  KW  - Fusarium austroafricanum
  KW  - Fusarium babinda
  KW  - Fusarium polyphialidicum
  KW  - gene genealogies
  KW  - morphology
  KW  - mycotoxins
  KW  - pathogenicity
  KW  - phylogenetic species recognition
  KW  - 1 new taxon
  UR  - http://dx.doi.org/
  N2  - Multilocus DNA sequence data were used to investigate species identity and diversity in two sister clades, the Fusarium concolor (FCOSC) and F. babinda species complexes.  The molecular phylogenetic results support the recognition of a novel Fusarium species within the FCOSC, which is described and illustrated here as F. austroafricanum sp. nov.   It was isolated as an endophyte of kikuyu grass associated with a putative mycotoxicosis of cattle and from plant debris in soil in South Africa.  Molecular phylogenetic analyses indicated it represents a genealogically exclusive lineage that is sister to F. concolor.  Of the 109 isolates analyzed, only four were received correctly identified to species and these included 1/46 F. concolor, 1/31 F. babinda and 2/3 F. anguioides.  The majority of the F. concolor and F. babinda isolates were received as F. polyphialidicum, which is a heterotypic synonym of the former species.  Previously documented from South America, Africa, Europe and Australia, our data show F. concolor is also present in North America.  The present study expands the known distribution of F. babinda in Australia to Asia, Europe and North America.  Fusarium austroafricanum is most similar morphologically to F. concolor and F. babinda but differs from the latter two species in producing: (i) much longer macroconidia in which the apical cell is blunt to slightly papillate and the basal cell is only slightly notched, (ii) macroconidia via microcycle conidiation on water agar, and (iii) head blight disease symptoms in inoculated spikelets of the susceptible wheat cultivar Norm.  BLASTn searches of the whole genome sequence of F. austroafricanum NRRL 53441 were conducted to predict mycotoxin potential, using genes known to be essential for the synthesis of several mycotoxins and biologically active metabolites.  Based on the presence of intact gene clusters that confer the ability to synthesize mycotoxins and pigments, we analyzed cracked corn kernel cultures of F. austroafricanum via liquid chromatography-mass spectrometry (LC-MS) but failed to detect these metabolites in vitro.  
  L3  - 
  JF  - Mycologia
  VL  - 
  IS  - 
  ER  -