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Citation for Study 631

Citation title: "Assessment of genetic relationships among isolates of Macrophomina phaseolina using a simplified AFLP technique and two different methods of analysis.".

This study was previously identified under the legacy study ID S461 (Status: Published).

Citation

Vandemark G., Martinez O., Pecina V., & Alvarado M. 2000. Assessment of genetic relationships among isolates of Macrophomina phaseolina using a simplified AFLP technique and two different methods of analysis. Mycologia, 92(4): 656-664.

Authors

Vandemark G.
Martinez O.
Pecina V.
Alvarado M.

Abstract

A simplified protocol for detecting amplified restriction fragment length polymorphisms (AFLPs) was used to evaluate genetic diversity among isolates of Macrophomina phaseolina collected from different hosts and locations. Fifteen different selective primer pairs were evaluated. No significant differences were observed among primer pairs, grouped based on the AT% of the selective nucleotides, for several parameters that define primer utility. Relationship matrices generated with each group of AFLP primers were highly correlated (r^2 > 0.92). Isolates were very diverse and could not clearly be grouped based on the geographic locations from which they were obtained. Genetic relationships among isolates were very robust. Relationships were determined using both the 'genetic similarity' method of Nei and Li and the 'genetic distance' method of Skroch. We conclude that both methods are equally effective for determining intraspecific genetic relationships when the majority of markers are polymorphic.

Keywords

agarose gel electrophoresis; genetic distance; genetic similarity; selective primers

External links

Other URL: http://www.jstor.org/stable/3761423

About this resource

Canonical resource URI: http://purl.org/phylo/treebase/phylows/study/TB2:S631
Other versions: Nexus NeXML

Show BibTeX reference

		@ARTICLE{TreeBASE2Ref17936,
	 author = {George  Vandemark and Octavio  Martinez and Victor  Pecina and Maria de Jesus  Alvarado},
	 title = {Assessment of genetic relationships among isolates of Macrophomina phaseolina using a simplified AFLP technique and two different methods of analysis.},
	 year = {2000},
	 keywords = {agarose gel electrophoresis; genetic distance; genetic similarity; selective primers },
	 doi = {},
	 url = {http://www.jstor.org/stable/3761423},
	 pmid = {},
	 journal = {Mycologia},
	 volume = {92},
	 number = {4},
	 pages = {656--664},
	 abstract = {A simplified protocol for detecting amplified restriction fragment length polymorphisms (AFLPs) was used to evaluate genetic diversity among isolates of Macrophomina phaseolina collected from different hosts and locations. Fifteen different selective primer pairs were evaluated. No significant differences were observed among primer pairs, grouped based on the AT% of the selective nucleotides, for several parameters that define primer utility. Relationship matrices generated with each group of AFLP primers were highly correlated (r^2 > 0.92). Isolates were very diverse and could not clearly be grouped based on the geographic locations from which they were obtained. Genetic relationships among isolates were very robust. Relationships were determined using both the 'genetic similarity' method of Nei and Li and the 'genetic distance' method of Skroch. We conclude that both methods are equally effective for determining intraspecific genetic relationships when the majority of markers are polymorphic.}
}

Show RIS reference

TY - JOUR
ID - 17936
AU - Vandemark,George
AU - Martinez,Octavio
AU - Pecina,Victor
AU - Alvarado,Maria de Jesus
T1 - Assessment of genetic relationships among isolates of Macrophomina phaseolina using a simplified AFLP technique and two different methods of analysis.
PY - 2000
KW - agarose gel electrophoresis; genetic distance; genetic similarity; selective primers
UR - http://www.jstor.org/stable/3761423
N2 - A simplified protocol for detecting amplified restriction fragment length polymorphisms (AFLPs) was used to evaluate genetic diversity among isolates of Macrophomina phaseolina collected from different hosts and locations. Fifteen different selective primer pairs were evaluated. No significant differences were observed among primer pairs, grouped based on the AT% of the selective nucleotides, for several parameters that define primer utility. Relationship matrices generated with each group of AFLP primers were highly correlated (r^2 > 0.92). Isolates were very diverse and could not clearly be grouped based on the geographic locations from which they were obtained. Genetic relationships among isolates were very robust. Relationships were determined using both the 'genetic similarity' method of Nei and Li and the 'genetic distance' method of Skroch. We conclude that both methods are equally effective for determining intraspecific genetic relationships when the majority of markers are polymorphic.
L3 -
JF - Mycologia
VL - 92
IS - 4
SP - 656
EP - 664
ER -