@ARTICLE{TreeBASE2Ref17846,
author = {Paul W. Tooley and Erin D. Goley and Marie M. Carras and Reid D. Frederick and Erin L. Weber and Gretchen A. Kuldau},
title = {Characterization of Claviceps species pathogenic on sorghum by sequence analysis of the beta-tubulin gene intron 3 region and EF-1 alpha gene intron 4.},
year = {2000},
keywords = {detection; ergot; PCR; phylogenetics; sugary disease; taxonomy },
doi = {},
url = {http://www.jstor.org/stable/3761739},
pmid = {},
journal = {Mycologia},
volume = {93},
number = {3},
pages = {541--551},
abstract = {The intron 3 region of the beta -tubulin gene, and intron 4 of the translation elongation factor gene were PCR-amplified, cloned, and sequenced to determine relationships among Claviceps species and characterize isolates of Claviceps causing ergot of sorghum in the USA and other countries. The beta -tubulin gene intron 3 region and intron 4 of the EF-1 alpha gene allowed clear differentiation of five species (C. africana, C. sorghicola, C. purpurea, C. fusiformis, and C. paspali), two of which (C. africana and C. sorghicola) are pathogens of sorghum, with almost no intraspecific variation observed among isolates. Claviceps isolates obtained from sorghum in the USA contained beta -tubulin gene intron 3 region sequences identical to those, of C. africana isolates from India, Australia, and South Africa. PCR primers were designed from unique sequences within the beta -tubulin intron 3 region that can differentiate the five Claviceps species used in this study. We describe primers that allow direct PCR detection of C. africana from honeydew produced on infected sorghum plants, providing a useful tool for analysis of field samples. The beta -tubulin gene intron 3 region and EF-1 alpha intron 4 should prove useful in phylogenetic and epidemiological studies of additional Claviceps species.}
}
Citation for Study 943
Citation title:
"Characterization of Claviceps species pathogenic on sorghum by sequence analysis of the beta-tubulin gene intron 3 region and EF-1 alpha gene intron 4.".
This study was previously identified under the legacy study ID S496
(Status: Published).
Citation
Tooley P., Goley E., Carras M., Frederick R., Weber E., & Kuldau G. 2000. Characterization of Claviceps species pathogenic on sorghum by sequence analysis of the beta-tubulin gene intron 3 region and EF-1 alpha gene intron 4. Mycologia, 93(3): 541-551.
Authors
-
Tooley P.
-
Goley E.
-
Carras M.
-
Frederick R.
-
Weber E.
-
Kuldau G.
Abstract
The intron 3 region of the beta -tubulin gene, and intron 4 of the translation elongation factor gene were PCR-amplified, cloned, and sequenced to determine relationships among Claviceps species and characterize isolates of Claviceps causing ergot of sorghum in the USA and other countries. The beta -tubulin gene intron 3 region and intron 4 of the EF-1 alpha gene allowed clear differentiation of five species (C. africana, C. sorghicola, C. purpurea, C. fusiformis, and C. paspali), two of which (C. africana and C. sorghicola) are pathogens of sorghum, with almost no intraspecific variation observed among isolates. Claviceps isolates obtained from sorghum in the USA contained beta -tubulin gene intron 3 region sequences identical to those, of C. africana isolates from India, Australia, and South Africa. PCR primers were designed from unique sequences within the beta -tubulin intron 3 region that can differentiate the five Claviceps species used in this study. We describe primers that allow direct PCR detection of C. africana from honeydew produced on infected sorghum plants, providing a useful tool for analysis of field samples. The beta -tubulin gene intron 3 region and EF-1 alpha intron 4 should prove useful in phylogenetic and epidemiological studies of additional Claviceps species.
Keywords
detection; ergot; PCR; phylogenetics; sugary disease; taxonomy
External links
About this resource
- Canonical resource URI:
http://purl.org/phylo/treebase/phylows/study/TB2:S943
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- Show BibTeX reference
@ARTICLE{TreeBASE2Ref17846,
author = {Paul W. Tooley and Erin D. Goley and Marie M. Carras and Reid D. Frederick and Erin L. Weber and Gretchen A. Kuldau},
title = {Characterization of Claviceps species pathogenic on sorghum by sequence analysis of the beta-tubulin gene intron 3 region and EF-1 alpha gene intron 4.},
year = {2000},
keywords = {detection; ergot; PCR; phylogenetics; sugary disease; taxonomy },
doi = {},
url = {http://www.jstor.org/stable/3761739},
pmid = {},
journal = {Mycologia},
volume = {93},
number = {3},
pages = {541--551},
abstract = {The intron 3 region of the beta -tubulin gene, and intron 4 of the translation elongation factor gene were PCR-amplified, cloned, and sequenced to determine relationships among Claviceps species and characterize isolates of Claviceps causing ergot of sorghum in the USA and other countries. The beta -tubulin gene intron 3 region and intron 4 of the EF-1 alpha gene allowed clear differentiation of five species (C. africana, C. sorghicola, C. purpurea, C. fusiformis, and C. paspali), two of which (C. africana and C. sorghicola) are pathogens of sorghum, with almost no intraspecific variation observed among isolates. Claviceps isolates obtained from sorghum in the USA contained beta -tubulin gene intron 3 region sequences identical to those, of C. africana isolates from India, Australia, and South Africa. PCR primers were designed from unique sequences within the beta -tubulin intron 3 region that can differentiate the five Claviceps species used in this study. We describe primers that allow direct PCR detection of C. africana from honeydew produced on infected sorghum plants, providing a useful tool for analysis of field samples. The beta -tubulin gene intron 3 region and EF-1 alpha intron 4 should prove useful in phylogenetic and epidemiological studies of additional Claviceps species.}
}
- Show RIS reference
TY - JOUR
ID - 17846
AU - Tooley,Paul W.
AU - Goley,Erin D.
AU - Carras,Marie M.
AU - Frederick,Reid D.
AU - Weber,Erin L.
AU - Kuldau,Gretchen A.
T1 - Characterization of Claviceps species pathogenic on sorghum by sequence analysis of the beta-tubulin gene intron 3 region and EF-1 alpha gene intron 4.
PY - 2000
KW - detection; ergot; PCR; phylogenetics; sugary disease; taxonomy
UR - http://www.jstor.org/stable/3761739
N2 - The intron 3 region of the beta -tubulin gene, and intron 4 of the translation elongation factor gene were PCR-amplified, cloned, and sequenced to determine relationships among Claviceps species and characterize isolates of Claviceps causing ergot of sorghum in the USA and other countries. The beta -tubulin gene intron 3 region and intron 4 of the EF-1 alpha gene allowed clear differentiation of five species (C. africana, C. sorghicola, C. purpurea, C. fusiformis, and C. paspali), two of which (C. africana and C. sorghicola) are pathogens of sorghum, with almost no intraspecific variation observed among isolates. Claviceps isolates obtained from sorghum in the USA contained beta -tubulin gene intron 3 region sequences identical to those, of C. africana isolates from India, Australia, and South Africa. PCR primers were designed from unique sequences within the beta -tubulin intron 3 region that can differentiate the five Claviceps species used in this study. We describe primers that allow direct PCR detection of C. africana from honeydew produced on infected sorghum plants, providing a useful tool for analysis of field samples. The beta -tubulin gene intron 3 region and EF-1 alpha intron 4 should prove useful in phylogenetic and epidemiological studies of additional Claviceps species.
L3 -
JF - Mycologia
VL - 93
IS - 3
SP - 541
EP - 551
ER -
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