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Citation for Study 10182

About Citation title: "Phylogenetic Placement, Genome Size, and GC-Content of the Liquid Hydrocarbon Producing Green Microalga Botryococcus Braunii var. Berkeley (Showa) (Chlorophyta)".
About This study was previously identified under the legacy study ID S2526 (Status: Published).

Citation

Weiss T., Johnston J., Fujisawa K., Sumimoto K., Okada S., Chappell J., & Devarenne T. 2010. Phylogenetic Placement, Genome Size, and GC-Content of the Liquid Hydrocarbon Producing Green Microalga Botryococcus Braunii var. Berkeley (Showa) (Chlorophyta). Journal of Phycology, 46(3): 534-540.

Authors

  • Weiss T.
  • Johnston J.
  • Fujisawa K.
  • Sumimoto K.
  • Okada S.
  • Chappell J.
  • Devarenne T.

Abstract

We report the genome size, the GC content and perform a phylogenetic analysis on Botryococcus braunii, K?tzing, a green, colony forming, hydrocarbon-rich alga that is an attractive source for biopetroleum. While the chemistry of the hydrocarbons produced by the B race of B. braunii has been studied for many years, there is a deficiency of information concerning the molecular biology of this alga. Additionally, there has been some discrepancy as to the phylogenetic placement of the Berkeley (or Showa) strain of the B race. In order to clarify its classification, we isolated the Berkeley strain nuclear small subunit (18S) rRNA gene and beta-actin cDNA and used these sequences for phylogenetic analysis to determine that the Berkeley strain belongs to the Trebouxiophyceae class. This is in agreement with other B races of B. braunii, indicating the Berkeley strain is a true B race of B. braunii. In order to better understand molecular aspects of B. braunii, we obtained the Berkeley strain genome size as a first step in genome sequencing. Using flow cytometry, we determined the B. braunii, Berkeley genome size to be 166.2 +/- 2.2 Mb. We also estimated the GC-content of the Berkeley strain as 54.4 +/- 1.2% for expressed gene sequences.

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About this resource

  • Canonical resource URI: http://purl.org/phylo/treebase/phylows/study/TB2:S10182
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