@ARTICLE{TreeBASE2Ref17364,
author = {Scott R. Santos and Derek J. Taylor and Mary Alice Coffroth},
title = {Genetic comparisons of freshly isolated vs. cultured symbiotic dinoflagellates: Implications for extrapolation to the intact symbiosis.},
year = {2001},
keywords = {culture; internal transcribed spacer; microsatellites; small subunit rDNA genes; Symbiodinium; symbiosis; symbiotic dinoflagellates; zooxanthellae},
doi = {10.1046/j.1529-8817.2001.00194.x},
url = {},
pmid = {},
journal = {Journal of Phycology},
volume = {37},
number = {5},
pages = {900--912},
abstract = {Zooxanthellae, algal symbionts in divergent marine invertebrate hosts, are a genetically heterogeneous group. All species descriptions and most physiological and infection studies of zooxanthellae have been conducted using cultured material, yet few studies to date have attempted to quantify the representation of cultures isolated from cnidarians to the in hospite zooxanthella populations from which they were established. Restriction Fragment Length Polymorphisms (RFLPs) of 18S-rDNA, Internal Transcribed Spacer (ITS) sequence data and microsatellites analyses were conducted to assess the relatedness between cultured zooxanthellae and the in hospite population from which they were isolated. RFLP data demonstrated cultures may represent either the numerically dominant symbiont or ones present in lower number. ITS sequences from zooxanthella cultures are disconcordant with ITS sequence identified from the in hospite zooxanthellae and microsatellites present in in hospite zooxanthella populations were absent from the corresponding cultures. Lastly, reexamination of the literature reveals examples of zooxanthella cultures being nonrepresentative of in hospite populations. These data suggest that, in most cases, cultures are a subset of the original in hospite population. Factors such as failing to homogenize bulk cultures prior to transfer, growth medium employed and the picking of single motile cells are thought to contribute to many zooxanthella cultures being nonrepresentative.}
}
Citation for Study 740
Citation title:
"Genetic comparisons of freshly isolated vs. cultured symbiotic dinoflagellates: Implications for extrapolation to the intact symbiosis.".
This study was previously identified under the legacy study ID S586
(Status: Published).
Citation
Santos S., Taylor D., & Coffroth M. 2001. Genetic comparisons of freshly isolated vs. cultured symbiotic dinoflagellates: Implications for extrapolation to the intact symbiosis. Journal of Phycology, 37(5): 900-912.
Authors
-
Santos S.
-
Taylor D.
-
Coffroth M.
Abstract
Zooxanthellae, algal symbionts in divergent marine invertebrate hosts, are a genetically heterogeneous group. All species descriptions and most physiological and infection studies of zooxanthellae have been conducted using cultured material, yet few studies to date have attempted to quantify the representation of cultures isolated from cnidarians to the in hospite zooxanthella populations from which they were established. Restriction Fragment Length Polymorphisms (RFLPs) of 18S-rDNA, Internal Transcribed Spacer (ITS) sequence data and microsatellites analyses were conducted to assess the relatedness between cultured zooxanthellae and the in hospite population from which they were isolated. RFLP data demonstrated cultures may represent either the numerically dominant symbiont or ones present in lower number. ITS sequences from zooxanthella cultures are disconcordant with ITS sequence identified from the in hospite zooxanthellae and microsatellites present in in hospite zooxanthella populations were absent from the corresponding cultures. Lastly, reexamination of the literature reveals examples of zooxanthella cultures being nonrepresentative of in hospite populations. These data suggest that, in most cases, cultures are a subset of the original in hospite population. Factors such as failing to homogenize bulk cultures prior to transfer, growth medium employed and the picking of single motile cells are thought to contribute to many zooxanthella cultures being nonrepresentative.
Keywords
culture; internal transcribed spacer; microsatellites; small subunit rDNA genes; Symbiodinium; symbiosis; symbiotic dinoflagellates; zooxanthellae
External links
About this resource
- Canonical resource URI:
http://purl.org/phylo/treebase/phylows/study/TB2:S740
- Other versions:
Nexus
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- Show BibTeX reference
@ARTICLE{TreeBASE2Ref17364,
author = {Scott R. Santos and Derek J. Taylor and Mary Alice Coffroth},
title = {Genetic comparisons of freshly isolated vs. cultured symbiotic dinoflagellates: Implications for extrapolation to the intact symbiosis.},
year = {2001},
keywords = {culture; internal transcribed spacer; microsatellites; small subunit rDNA genes; Symbiodinium; symbiosis; symbiotic dinoflagellates; zooxanthellae},
doi = {10.1046/j.1529-8817.2001.00194.x},
url = {},
pmid = {},
journal = {Journal of Phycology},
volume = {37},
number = {5},
pages = {900--912},
abstract = {Zooxanthellae, algal symbionts in divergent marine invertebrate hosts, are a genetically heterogeneous group. All species descriptions and most physiological and infection studies of zooxanthellae have been conducted using cultured material, yet few studies to date have attempted to quantify the representation of cultures isolated from cnidarians to the in hospite zooxanthella populations from which they were established. Restriction Fragment Length Polymorphisms (RFLPs) of 18S-rDNA, Internal Transcribed Spacer (ITS) sequence data and microsatellites analyses were conducted to assess the relatedness between cultured zooxanthellae and the in hospite population from which they were isolated. RFLP data demonstrated cultures may represent either the numerically dominant symbiont or ones present in lower number. ITS sequences from zooxanthella cultures are disconcordant with ITS sequence identified from the in hospite zooxanthellae and microsatellites present in in hospite zooxanthella populations were absent from the corresponding cultures. Lastly, reexamination of the literature reveals examples of zooxanthella cultures being nonrepresentative of in hospite populations. These data suggest that, in most cases, cultures are a subset of the original in hospite population. Factors such as failing to homogenize bulk cultures prior to transfer, growth medium employed and the picking of single motile cells are thought to contribute to many zooxanthella cultures being nonrepresentative.}
}
- Show RIS reference
TY - JOUR
ID - 17364
AU - Santos,Scott R.
AU - Taylor,Derek J.
AU - Coffroth,Mary Alice
T1 - Genetic comparisons of freshly isolated vs. cultured symbiotic dinoflagellates: Implications for extrapolation to the intact symbiosis.
PY - 2001
KW - culture; internal transcribed spacer; microsatellites; small subunit rDNA genes; Symbiodinium; symbiosis; symbiotic dinoflagellates; zooxanthellae
UR - http://dx.doi.org/10.1046/j.1529-8817.2001.00194.x
N2 - Zooxanthellae, algal symbionts in divergent marine invertebrate hosts, are a genetically heterogeneous group. All species descriptions and most physiological and infection studies of zooxanthellae have been conducted using cultured material, yet few studies to date have attempted to quantify the representation of cultures isolated from cnidarians to the in hospite zooxanthella populations from which they were established. Restriction Fragment Length Polymorphisms (RFLPs) of 18S-rDNA, Internal Transcribed Spacer (ITS) sequence data and microsatellites analyses were conducted to assess the relatedness between cultured zooxanthellae and the in hospite population from which they were isolated. RFLP data demonstrated cultures may represent either the numerically dominant symbiont or ones present in lower number. ITS sequences from zooxanthella cultures are disconcordant with ITS sequence identified from the in hospite zooxanthellae and microsatellites present in in hospite zooxanthella populations were absent from the corresponding cultures. Lastly, reexamination of the literature reveals examples of zooxanthella cultures being nonrepresentative of in hospite populations. These data suggest that, in most cases, cultures are a subset of the original in hospite population. Factors such as failing to homogenize bulk cultures prior to transfer, growth medium employed and the picking of single motile cells are thought to contribute to many zooxanthella cultures being nonrepresentative.
L3 - 10.1046/j.1529-8817.2001.00194.x
JF - Journal of Phycology
VL - 37
IS - 5
SP - 900
EP - 912
ER -