@ARTICLE{TreeBASE2Ref19585,
author = {Nicolas Feau and Agathe Vialle and Mathieu Allaire and Wolfgang Maier and Richard C. Hamelin},
title = {DNA barcoding in the rust genus Chrysomyxa and its implications for the phylogeny of the genus},
year = {2011},
keywords = {Phylogeny, fungal pathogen, species complex, Chrysomyxa needle rusts, cone rusts, DNA barcode, mitochondrial heteroplasmon},
doi = {},
url = {http://},
pmid = {},
journal = {Mycologia},
volume = {},
number = {},
pages = {},
abstract = {Chrysomyxa rusts are fungal pathogens widely present in the boreal forest. Taxonomic delimitation and precise species identification are difficult within this genus since several species display similar morphological features. We applied a DNA barcode system based on the ribosomal internal transcribed spacer region (ITS), large subunit (28S) ribosomal RNA gene, mitochondrial cytochrome oxidase 1 (CO1) and mitochondrial NADH dehydrogenase subunit 6 (nad6) in 86 strains from 16 different Chrysomyxa species, including members of the Chrysomyxa ledi species complex. The nuclear ITS and 28S loci revealed higher resolving power than the mitochondrial genes. Amplification of the full CO1 barcode region failed due to the presence of introns limiting the data set obtained with this barcode. In most cases, the ITS barcodes were in agreement with taxonomic species based on phenotypic characters. Nevertheless, we observed genetically distinct (different DNA barcodes) lineages within Chrysomyxa pyrolae and Chrysomyxa rhododendri, providing some evidence for allopatric speciation within these morphologically defined species. This finding, together with the observed pattern of host specificities of the studied rust fungi suggest that species diversification within the C. ledi species complex might be governed by a set of factors, such as specialisation to certain Ericaceae species as telial hosts and, to a lesser extent, specialisation to different spruce species as aecial hosts. Moreover, allopatric speciation by geographic disruption of species also seems to take place. Unexpectedly, when our data were integrated into a broader phylogenetic framework, the Chrysomyxa genus was not resolved as a monophyletic group. Indeed, the spruce cone rusts C. pyrolae and C. monesis coalesced with the pine needle rusts belonging to the genus Coleosporium, whereas the microcyclic species Chrysomyxa weirii was embedded within a clade comprising the genus Melampsora. }
}
Citation for Study 11350
Citation title:
"DNA barcoding in the rust genus Chrysomyxa and its implications for the phylogeny of the genus".
Study name:
"DNA barcoding in the rust genus Chrysomyxa and its implications for the phylogeny of the genus".
This study is part of submission 11340
(Status: Published).
Citation
Feau N., Vialle A., Allaire M., Maier W., & Hamelin R. 2011. DNA barcoding in the rust genus Chrysomyxa and its implications for the phylogeny of the genus. Mycologia, .
Authors
-
Feau N.
(submitter)
-
Vialle A.
-
Allaire M.
-
Maier W.
-
Hamelin R.
Abstract
Chrysomyxa rusts are fungal pathogens widely present in the boreal forest. Taxonomic delimitation and precise species identification are difficult within this genus since several species display similar morphological features. We applied a DNA barcode system based on the ribosomal internal transcribed spacer region (ITS), large subunit (28S) ribosomal RNA gene, mitochondrial cytochrome oxidase 1 (CO1) and mitochondrial NADH dehydrogenase subunit 6 (nad6) in 86 strains from 16 different Chrysomyxa species, including members of the Chrysomyxa ledi species complex. The nuclear ITS and 28S loci revealed higher resolving power than the mitochondrial genes. Amplification of the full CO1 barcode region failed due to the presence of introns limiting the data set obtained with this barcode. In most cases, the ITS barcodes were in agreement with taxonomic species based on phenotypic characters. Nevertheless, we observed genetically distinct (different DNA barcodes) lineages within Chrysomyxa pyrolae and Chrysomyxa rhododendri, providing some evidence for allopatric speciation within these morphologically defined species. This finding, together with the observed pattern of host specificities of the studied rust fungi suggest that species diversification within the C. ledi species complex might be governed by a set of factors, such as specialisation to certain Ericaceae species as telial hosts and, to a lesser extent, specialisation to different spruce species as aecial hosts. Moreover, allopatric speciation by geographic disruption of species also seems to take place. Unexpectedly, when our data were integrated into a broader phylogenetic framework, the Chrysomyxa genus was not resolved as a monophyletic group. Indeed, the spruce cone rusts C. pyrolae and C. monesis coalesced with the pine needle rusts belonging to the genus Coleosporium, whereas the microcyclic species Chrysomyxa weirii was embedded within a clade comprising the genus Melampsora.
Keywords
Phylogeny, fungal pathogen, species complex, Chrysomyxa needle rusts, cone rusts, DNA barcode, mitochondrial heteroplasmon
External links
About this resource
- Canonical resource URI:
http://purl.org/phylo/treebase/phylows/study/TB2:S11350
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- Show BibTeX reference
@ARTICLE{TreeBASE2Ref19585,
author = {Nicolas Feau and Agathe Vialle and Mathieu Allaire and Wolfgang Maier and Richard C. Hamelin},
title = {DNA barcoding in the rust genus Chrysomyxa and its implications for the phylogeny of the genus},
year = {2011},
keywords = {Phylogeny, fungal pathogen, species complex, Chrysomyxa needle rusts, cone rusts, DNA barcode, mitochondrial heteroplasmon},
doi = {},
url = {http://},
pmid = {},
journal = {Mycologia},
volume = {},
number = {},
pages = {},
abstract = {Chrysomyxa rusts are fungal pathogens widely present in the boreal forest. Taxonomic delimitation and precise species identification are difficult within this genus since several species display similar morphological features. We applied a DNA barcode system based on the ribosomal internal transcribed spacer region (ITS), large subunit (28S) ribosomal RNA gene, mitochondrial cytochrome oxidase 1 (CO1) and mitochondrial NADH dehydrogenase subunit 6 (nad6) in 86 strains from 16 different Chrysomyxa species, including members of the Chrysomyxa ledi species complex. The nuclear ITS and 28S loci revealed higher resolving power than the mitochondrial genes. Amplification of the full CO1 barcode region failed due to the presence of introns limiting the data set obtained with this barcode. In most cases, the ITS barcodes were in agreement with taxonomic species based on phenotypic characters. Nevertheless, we observed genetically distinct (different DNA barcodes) lineages within Chrysomyxa pyrolae and Chrysomyxa rhododendri, providing some evidence for allopatric speciation within these morphologically defined species. This finding, together with the observed pattern of host specificities of the studied rust fungi suggest that species diversification within the C. ledi species complex might be governed by a set of factors, such as specialisation to certain Ericaceae species as telial hosts and, to a lesser extent, specialisation to different spruce species as aecial hosts. Moreover, allopatric speciation by geographic disruption of species also seems to take place. Unexpectedly, when our data were integrated into a broader phylogenetic framework, the Chrysomyxa genus was not resolved as a monophyletic group. Indeed, the spruce cone rusts C. pyrolae and C. monesis coalesced with the pine needle rusts belonging to the genus Coleosporium, whereas the microcyclic species Chrysomyxa weirii was embedded within a clade comprising the genus Melampsora. }
}
- Show RIS reference
TY - JOUR
ID - 19585
AU - Feau,Nicolas
AU - Vialle,Agathe
AU - Allaire,Mathieu
AU - Maier,Wolfgang
AU - Hamelin,Richard C.
T1 - DNA barcoding in the rust genus Chrysomyxa and its implications for the phylogeny of the genus
PY - 2011
KW - Phylogeny
KW - fungal pathogen
KW - species complex
KW - Chrysomyxa needle rusts
KW - cone rusts
KW - DNA barcode
KW - mitochondrial heteroplasmon
UR - http://dx.doi.org/
N2 - Chrysomyxa rusts are fungal pathogens widely present in the boreal forest. Taxonomic delimitation and precise species identification are difficult within this genus since several species display similar morphological features. We applied a DNA barcode system based on the ribosomal internal transcribed spacer region (ITS), large subunit (28S) ribosomal RNA gene, mitochondrial cytochrome oxidase 1 (CO1) and mitochondrial NADH dehydrogenase subunit 6 (nad6) in 86 strains from 16 different Chrysomyxa species, including members of the Chrysomyxa ledi species complex. The nuclear ITS and 28S loci revealed higher resolving power than the mitochondrial genes. Amplification of the full CO1 barcode region failed due to the presence of introns limiting the data set obtained with this barcode. In most cases, the ITS barcodes were in agreement with taxonomic species based on phenotypic characters. Nevertheless, we observed genetically distinct (different DNA barcodes) lineages within Chrysomyxa pyrolae and Chrysomyxa rhododendri, providing some evidence for allopatric speciation within these morphologically defined species. This finding, together with the observed pattern of host specificities of the studied rust fungi suggest that species diversification within the C. ledi species complex might be governed by a set of factors, such as specialisation to certain Ericaceae species as telial hosts and, to a lesser extent, specialisation to different spruce species as aecial hosts. Moreover, allopatric speciation by geographic disruption of species also seems to take place. Unexpectedly, when our data were integrated into a broader phylogenetic framework, the Chrysomyxa genus was not resolved as a monophyletic group. Indeed, the spruce cone rusts C. pyrolae and C. monesis coalesced with the pine needle rusts belonging to the genus Coleosporium, whereas the microcyclic species Chrysomyxa weirii was embedded within a clade comprising the genus Melampsora.
L3 -
JF - Mycologia
VL -
IS -
ER -