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Citation for Study 14758

Citation title: "Identification, organization, and distribution of the mating type locus in fusaria that cause soybean sudden death syndrome and bean root rot".

Study name: "Identification, organization, and distribution of the mating type locus in fusaria that cause soybean sudden death syndrome and bean root rot".

This study is part of submission 14758 (Status: Published).

Citation

Hughes T.J., O'donnell K., Rooney A., Sink S., Scandiani M.M., Luque A., Bhattacharyya M.K., & Huang X. 2013. Identification, organization, and distribution of the mating type locus in fusaria that cause soybean sudden death syndrome and bean root rot. Mycologia, .

Authors

• Hughes T.J.
• O'donnell K. 309-681-6383
• Rooney A.
• Sink S. (submitter)
• Scandiani M.M.
• Luque A.
• Bhattacharyya M.K.
• Huang X.

Abstract

Fusarium tucumaniae is the only known sexually reproducing species among the seven closely related fusaria that cause soybean sudden death syndrome (SDS) or bean root rot (BRR). In a previous study, laboratory mating of F. tucumaniae yielded recombinant ascospore progeny, but required two mating-compatible strains, indicating that it is heterothallic. To assess the reproductive mode of the other SDS and BRR fusaria, and their potential for mating, whole-genome sequences of two SDS and one BRR pathogen were analyzed to characterize their mating type (MAT) loci. This bioinformatic approach identified a MAT1-1 idiomorph in F. virguliforme NRRL 22292 and MAT1-2 idiomorphs in F. tucumaniae NRRL 34546 and F. azukicola NRRL 54364. Alignments of the MAT loci were used to design PCR primers within the conserved regions of the flanking genes APN1 and SLA2, which enabled primer walking to obtain nearly complete sequences of the MAT region for six MAT1-1 and five MAT1-2 SDS/BRR fusaria. As expected, the sequences of the highly divergent 4.7 kb MAT1-1 and 3.7 kb MAT1-2 idiomorphs were unalignable. However, the sequences of the respective idiomorphs and those that flank MAT1-1 and MAT1-2, were highly conserved. In addition to three genes at MAT1-1 (MAT1-1-1, MAT1-1-2 and MAT1-1-3) and two at MAT1-2 (MAT1-2-1 and MAT1-2-3), the MAT loci of SDS/BRR fusaria appeared to have expanded to include a putative gene predicted to encode for a 252 amino acid protein of unknown function. Alignments of the MAT1-1-3 and MAT1-2-1 sequences were used to design a multiplex PCR assay for the MAT loci. This assay was used to screen DNA from 439 SDS/BRR isolates, which revealed that each isolate possessed MAT1-1 or MAT1-2, consistent with heterothallism. Both idiomorphs were represented among isolates of F. azukicola, F. brasiliense, F. phaseoli and F. tucumaniae, whereas isolates of F. virguliforme and F. cuneirostrum were only MAT1-1 and F. crassistipitatum were only MAT1-2. Although phylogenies inferred from MAT were mostly unresolved, consistent with their hypothesized relatively recent evolutionary origin, analyses of the MAT locus sequences and six other loci suggested that Fusarium sp. NRRL 22411 may represent an interspecific hybrid between F. brasiliense and F. phaseoli-like parents.

Keywords

Fusarium solani, clade 2, α-box, heterothallic, high mobility group, idiomorph, interspecific hybridization, MAT1-2-3, PCR assay, whole genome, disease management

External links

About this resource

• Canonical resource URI: http://purl.org/phylo/treebase/phylows/study/TB2:S14758
• Other versions: Nexus NeXML
• Show BibTeX reference

  		@ARTICLE{TreeBASE2Ref22413,
  	 author = {Teresa J. Hughes and Kerry  O'Donnell and Alejandro  Rooney and stacy  sink and Mar?a Mercedes Scandiani and Alicia  Luque and Madan K. Bhattacharyya and Xiaoqui  Huang},
  	 title = {Identification, organization, and distribution of the mating type locus in fusaria that cause soybean sudden death syndrome and bean root rot},
  	 year = {2013},
  	 keywords = {Fusarium solani, clade 2, α-box, heterothallic, high mobility group, idiomorph, interspecific hybridization, MAT1-2-3, PCR assay, whole genome, disease management },
  	 doi = {},
  	 url = {http://},
  	 pmid = {},
  	 journal = {Mycologia},
  	 volume = {},
  	 number = {},
  	 pages = {},
  	 abstract = {Fusarium tucumaniae is the only known sexually reproducing species among the seven closely related fusaria that cause soybean sudden death syndrome (SDS) or bean root rot (BRR).   In a previous study, laboratory mating of F. tucumaniae yielded recombinant ascospore progeny, but required two mating-compatible strains, indicating that it is heterothallic.  To assess the reproductive mode of the other SDS and BRR fusaria, and their potential for mating, whole-genome sequences of two SDS and one BRR pathogen were analyzed to characterize their mating type (MAT) loci.  This bioinformatic approach identified a MAT1-1 idiomorph in F. virguliforme NRRL 22292 and MAT1-2 idiomorphs in F. tucumaniae NRRL 34546 and F. azukicola NRRL 54364.  Alignments of the MAT loci were used to design PCR primers within the conserved regions of the flanking genes APN1 and SLA2, which enabled primer walking to obtain nearly complete sequences of the MAT region for six MAT1-1 and five MAT1-2 SDS/BRR fusaria.  As expected, the sequences of the highly divergent 4.7 kb MAT1-1 and 3.7 kb MAT1-2 idiomorphs were unalignable. However, the sequences of the respective idiomorphs and those that flank MAT1-1 and MAT1-2, were highly conserved.  In addition to three genes at MAT1-1 (MAT1-1-1, MAT1-1-2 and MAT1-1-3) and two at MAT1-2 (MAT1-2-1 and MAT1-2-3), the MAT loci of SDS/BRR fusaria appeared to have expanded to include a putative gene predicted to encode for a 252 amino acid protein of unknown function. Alignments of the MAT1-1-3 and MAT1-2-1 sequences were used to design a multiplex PCR assay for the MAT loci.  This assay was used to screen DNA from 439 SDS/BRR isolates, which revealed that each isolate possessed MAT1-1 or MAT1-2, consistent with heterothallism.  Both idiomorphs were represented among isolates of F. azukicola, F. brasiliense, F. phaseoli and F. tucumaniae, whereas isolates of F. virguliforme and F. cuneirostrum were only MAT1-1 and F. crassistipitatum were only MAT1-2.  Although phylogenies inferred from MAT were mostly unresolved, consistent with their hypothesized relatively recent evolutionary origin, analyses of the MAT locus sequences and six other loci suggested that Fusarium sp. NRRL 22411 may represent an interspecific hybrid between F. brasiliense and F. phaseoli-like parents.  }
  }
  		

• Show RIS reference

  		TY  - JOUR
  ID  - 22413
  AU  - Hughes,Teresa J.
  AU  - O'Donnell,Kerry 
  AU  - Rooney,Alejandro 
  AU  - sink,stacy 
  AU  - Scandiani,Mar?a Mercedes
  AU  - Luque,Alicia 
  AU  - Bhattacharyya,Madan K.
  AU  - Huang,Xiaoqui 
  T1  - Identification, organization, and distribution of the mating type locus in fusaria that cause soybean sudden death syndrome and bean root rot
  PY  - 2013
  KW  - Fusarium solani
  KW  - clade 2
  KW  - α-box
  KW  - heterothallic
  KW  - high mobility group
  KW  - idiomorph
  KW  - interspecific hybridization
  KW  - MAT1-2-3
  KW  - PCR assay
  KW  - whole genome
  KW  - disease management 
  UR  - http://dx.doi.org/
  N2  - Fusarium tucumaniae is the only known sexually reproducing species among the seven closely related fusaria that cause soybean sudden death syndrome (SDS) or bean root rot (BRR).   In a previous study, laboratory mating of F. tucumaniae yielded recombinant ascospore progeny, but required two mating-compatible strains, indicating that it is heterothallic.  To assess the reproductive mode of the other SDS and BRR fusaria, and their potential for mating, whole-genome sequences of two SDS and one BRR pathogen were analyzed to characterize their mating type (MAT) loci.  This bioinformatic approach identified a MAT1-1 idiomorph in F. virguliforme NRRL 22292 and MAT1-2 idiomorphs in F. tucumaniae NRRL 34546 and F. azukicola NRRL 54364.  Alignments of the MAT loci were used to design PCR primers within the conserved regions of the flanking genes APN1 and SLA2, which enabled primer walking to obtain nearly complete sequences of the MAT region for six MAT1-1 and five MAT1-2 SDS/BRR fusaria.  As expected, the sequences of the highly divergent 4.7 kb MAT1-1 and 3.7 kb MAT1-2 idiomorphs were unalignable. However, the sequences of the respective idiomorphs and those that flank MAT1-1 and MAT1-2, were highly conserved.  In addition to three genes at MAT1-1 (MAT1-1-1, MAT1-1-2 and MAT1-1-3) and two at MAT1-2 (MAT1-2-1 and MAT1-2-3), the MAT loci of SDS/BRR fusaria appeared to have expanded to include a putative gene predicted to encode for a 252 amino acid protein of unknown function. Alignments of the MAT1-1-3 and MAT1-2-1 sequences were used to design a multiplex PCR assay for the MAT loci.  This assay was used to screen DNA from 439 SDS/BRR isolates, which revealed that each isolate possessed MAT1-1 or MAT1-2, consistent with heterothallism.  Both idiomorphs were represented among isolates of F. azukicola, F. brasiliense, F. phaseoli and F. tucumaniae, whereas isolates of F. virguliforme and F. cuneirostrum were only MAT1-1 and F. crassistipitatum were only MAT1-2.  Although phylogenies inferred from MAT were mostly unresolved, consistent with their hypothesized relatively recent evolutionary origin, analyses of the MAT locus sequences and six other loci suggested that Fusarium sp. NRRL 22411 may represent an interspecific hybrid between F. brasiliense and F. phaseoli-like parents.  
  L3  - 
  JF  - Mycologia
  VL  - 
  IS  - 
  ER  -