@ARTICLE{TreeBASE2Ref22544,
author = {Sung-Jin Cho and Yvonne Valles and David A. Weisblat},
title = {Differential expression of conserved germ line markers, and delayed segregation of male and female primordial germ cells, in a hermaphrodite, the leech Helobdella},
year = {2013},
keywords = {},
doi = {},
url = {http://},
pmid = {},
journal = {Molecular Biology and Evolution},
volume = {},
number = {},
pages = {},
abstract = {In sexually reproducing animals, primordial germ cells (PGCs) are often set aside
early in embryogenesis, a strategy which minimizes the risk of genomic damage associated
with replication and mitosis during the cell cycle. Here, we have used germ line markers
(piwi, vasa and nanos) and micro-injected cell lineage tracers to show that PGC specification
in the leech genus Helobdella follows a different scenario: in this hermaphrodite, the male
and female PGCs segregate from somatic lineages only after more than 20 rounds of zygotic
mitosis; the male and female PGCs share the same (mesodermal) cell lineage for 19 rounds of
zygotic mitosis. Moreover, while all three markers are expressed in both male and female
reproductive tissues of the adult, they are expressed differentially between the male and
female PGCs of the developing embryo: piwi and vasa are expressed preferentially in female
PGCs at a time when nanos is expressed preferentially in male PGCs. A priori, the delayed
segregation of male and female PGCs from somatic tissues and from one another increases
the probability of mutations affecting both male and female PGCs of a given individual. We
speculate that this suite of features, combined with a capacity for self-fertilization, may
contribute to the dramatically rearranged genome of Helobdella robusta relative to other
animals.
}
}
Citation for Study 14911
Citation title:
"Differential expression of conserved germ line markers, and delayed segregation of male and female primordial germ cells, in a hermaphrodite, the leech Helobdella".
Study name:
"Differential expression of conserved germ line markers, and delayed segregation of male and female primordial germ cells, in a hermaphrodite, the leech Helobdella".
This study is part of submission 14911
(Status: Published).
Citation
Cho S., Valles Y., & Weisblat D. 2013. Differential expression of conserved germ line markers, and delayed segregation of male and female primordial germ cells, in a hermaphrodite, the leech Helobdella. Molecular Biology and Evolution, .
Authors
-
Cho S.
-
Valles Y.
(submitter)
-
Weisblat D.
Abstract
In sexually reproducing animals, primordial germ cells (PGCs) are often set aside
early in embryogenesis, a strategy which minimizes the risk of genomic damage associated
with replication and mitosis during the cell cycle. Here, we have used germ line markers
(piwi, vasa and nanos) and micro-injected cell lineage tracers to show that PGC specification
in the leech genus Helobdella follows a different scenario: in this hermaphrodite, the male
and female PGCs segregate from somatic lineages only after more than 20 rounds of zygotic
mitosis; the male and female PGCs share the same (mesodermal) cell lineage for 19 rounds of
zygotic mitosis. Moreover, while all three markers are expressed in both male and female
reproductive tissues of the adult, they are expressed differentially between the male and
female PGCs of the developing embryo: piwi and vasa are expressed preferentially in female
PGCs at a time when nanos is expressed preferentially in male PGCs. A priori, the delayed
segregation of male and female PGCs from somatic tissues and from one another increases
the probability of mutations affecting both male and female PGCs of a given individual. We
speculate that this suite of features, combined with a capacity for self-fertilization, may
contribute to the dramatically rearranged genome of Helobdella robusta relative to other
animals.
External links
About this resource
- Canonical resource URI:
http://purl.org/phylo/treebase/phylows/study/TB2:S14911
- Other versions:
Nexus
NeXML
- Show BibTeX reference
@ARTICLE{TreeBASE2Ref22544,
author = {Sung-Jin Cho and Yvonne Valles and David A. Weisblat},
title = {Differential expression of conserved germ line markers, and delayed segregation of male and female primordial germ cells, in a hermaphrodite, the leech Helobdella},
year = {2013},
keywords = {},
doi = {},
url = {http://},
pmid = {},
journal = {Molecular Biology and Evolution},
volume = {},
number = {},
pages = {},
abstract = {In sexually reproducing animals, primordial germ cells (PGCs) are often set aside
early in embryogenesis, a strategy which minimizes the risk of genomic damage associated
with replication and mitosis during the cell cycle. Here, we have used germ line markers
(piwi, vasa and nanos) and micro-injected cell lineage tracers to show that PGC specification
in the leech genus Helobdella follows a different scenario: in this hermaphrodite, the male
and female PGCs segregate from somatic lineages only after more than 20 rounds of zygotic
mitosis; the male and female PGCs share the same (mesodermal) cell lineage for 19 rounds of
zygotic mitosis. Moreover, while all three markers are expressed in both male and female
reproductive tissues of the adult, they are expressed differentially between the male and
female PGCs of the developing embryo: piwi and vasa are expressed preferentially in female
PGCs at a time when nanos is expressed preferentially in male PGCs. A priori, the delayed
segregation of male and female PGCs from somatic tissues and from one another increases
the probability of mutations affecting both male and female PGCs of a given individual. We
speculate that this suite of features, combined with a capacity for self-fertilization, may
contribute to the dramatically rearranged genome of Helobdella robusta relative to other
animals.
}
}
- Show RIS reference
TY - JOUR
ID - 22544
AU - Cho,Sung-Jin
AU - Valles,Yvonne
AU - Weisblat,David A.
T1 - Differential expression of conserved germ line markers, and delayed segregation of male and female primordial germ cells, in a hermaphrodite, the leech Helobdella
PY - 2013
KW -
UR - http://dx.doi.org/
N2 - In sexually reproducing animals, primordial germ cells (PGCs) are often set aside
early in embryogenesis, a strategy which minimizes the risk of genomic damage associated
with replication and mitosis during the cell cycle. Here, we have used germ line markers
(piwi, vasa and nanos) and micro-injected cell lineage tracers to show that PGC specification
in the leech genus Helobdella follows a different scenario: in this hermaphrodite, the male
and female PGCs segregate from somatic lineages only after more than 20 rounds of zygotic
mitosis; the male and female PGCs share the same (mesodermal) cell lineage for 19 rounds of
zygotic mitosis. Moreover, while all three markers are expressed in both male and female
reproductive tissues of the adult, they are expressed differentially between the male and
female PGCs of the developing embryo: piwi and vasa are expressed preferentially in female
PGCs at a time when nanos is expressed preferentially in male PGCs. A priori, the delayed
segregation of male and female PGCs from somatic tissues and from one another increases
the probability of mutations affecting both male and female PGCs of a given individual. We
speculate that this suite of features, combined with a capacity for self-fertilization, may
contribute to the dramatically rearranged genome of Helobdella robusta relative to other
animals.
L3 -
JF - Molecular Biology and Evolution
VL -
IS -
ER -