CiteULike CiteULike
Delicious Delicious
Connotea Connotea

Citation for Study 11416

About Citation title: "What does it take to resolve relationships and to identify species with molecular markers? An example from the epiphytic Rhipsalideae (Cactaceae)".
About Study name: "What does it take to resolve relationships and to identify species with molecular markers? An example from the epiphytic Rhipsalideae (Cactaceae)".
About This study is part of submission 11406 (Status: Published).

Citation

Korotkova N., Borsch T., Quandt D., Taylor N.P., Müller K.F., & Barthlott W. 2011. What does it take to resolve relationships and to identify species with molecular markers? An example from the epiphytic Rhipsalideae (Cactaceae). American Journal of Botany, 98(9): 1549–1572.

Authors

  • Korotkova N. (submitter) Phone 004903083859962
  • Borsch T.
  • Quandt D.
  • Taylor N.P.
  • Müller K.F.
  • Barthlott W.

Abstract

• Premise of the study: The Cactaceae are a major New World plant family and popular in horticulture. Still, taxonomic units and species limits have been diffi cult to defi ne, and molecular phylogenetic studies so far have yielded largely unresolved trees, so relationships within Cactaceae remain insuffi ciently understood. This study focuses on the predominantly epiphytic tribe Rhipsalideae and evaluates the utility of a spectrum of plastid genomic regions. • Methods: We present a phylogenetic study including 52 of the 53 Rhipsalideae species and all the infraspecifi c taxa. Seven regions ( trnK intron, matK , rbcL , rps3 - rpl16 , rpl16 intron, psbA-trnH , trnQ-rps16 ), ca. 5600 nucleotides (nt) were sequenced per sample. The regions used were evaluated for their phylogenetic performance and performance in DNA-based species recognition based on operational taxonomic units (OTUs) defi ned beforehand. • Key results: The Rhipsalideae are monophyletic and contain fi ve clades that correspond to the genera Rhipsalis , Lepismium , Schlumbergera , Hatiora , and Rhipsalidopsis . The species-level tree was well resolved and supported; the rpl16 and trnK introns yielded the best phylogenetic signal. Although the psbA-trnH and trnQ-rps16 spacers were the most successful individual regions for OTU identifi cation, their success rate did not signifi cantly exceed 70%. The highest OTU identifi cation rate of 97% was found using the combination of psbA-trnH, rps3-rpl16, trnK intron, and trnQ-rps16 as a minimum possible marker length (ca. 1660 nt). • Conclusions : The phylogenetic performance of a marker is not determined by the level of sequence variability, and species discrimination power does not necessarily correlate with phylogenetic utility.

Keywords

Cactaceae; DNA barcoding; Hatiora; inversion; Lepismium; Rhipsalidopsis; Rhipsalis; Schlumbergera; species-level phylogeny; taxonomy

External links

About this resource

  • Canonical resource URI: http://purl.org/phylo/treebase/phylows/study/TB2:S11416
  • Other versions: Download Reconstructed NEXUS File Nexus Download NeXML File NeXML
  • Show BibTeX reference
  • Show RIS reference