TreeBASE Search-Citation for study 13716

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Citation for Study 13716

Citation title: "Spatiotemporal reconstruction of the Aquilegia rapid radiation through next-generation sequencing of rapidly evolving cpDNA regions.".

Study name: "Spatiotemporal reconstruction of the Aquilegia rapid radiation through next-generation sequencing of rapidly evolving cpDNA regions.".

This study is part of submission 13716 (Status: Published).

Citation

Fior S., Li M., Oxelman B., Viola R., Hodges S.A., Ometto L., & Varotto C. 2013. Spatiotemporal reconstruction of the Aquilegia rapid radiation through next-generation sequencing of rapidly evolving cpDNA regions. New Phytologist, 198: 579-592.

Authors

Fior S. (submitter)
Li M.
Oxelman B.
Viola R.
Hodges S.A.
Ometto L.
Varotto C.

Abstract

Aquilegia is a well-known model system in the field of evolutionary biology, but obtaining a resolved and well-supported phylogenetic reconstruction for the genus has been hindered by its recent and rapid diversification. Here, we applied 454 next-generation sequencing to PCR amplicons of 21 of the most rap- idly evolving regions of the plastome to generate c. 24 kb of sequences from each of 84 indi- viduals from throughout the genus. The resulting phylogeny has well-supported resolution of the main lineages of the genus, although recent diversification such as in the European taxa remains unresolved. By producing a chronogram of the whole Ranunculaceae family based on published data, we inferred cali- bration points for dating the Aquilegia radiation. The genus originated in the upper Miocene c. 6.9 million yr ago (Ma) in Eastern Asia, and diversification occurred c. 4.8 Ma with the split of two main clades, one colonizing North America, and the other Western Eurasia through the mountains of Central Asia. This was followed by a back-to-Asia migration, originating from the European stock using a North Asian route. These results provide the first backbone phylogeny and spatiotemporal reconstruction of the Aquilegia radiation, and constitute a robust framework to address the adaptative nature of speciation within the group.

Keywords

Aquilegia, Next-generation sequencing, Ranunculaceae molecular dat- ing, rapidly evolving noncoding cpDNA, recent rapid radiation, Roche 454.

External links

About this resource

Canonical resource URI: http://purl.org/phylo/treebase/phylows/study/TB2:S13716
Other versions: Nexus NeXML

Show BibTeX reference

		@ARTICLE{TreeBASE2Ref22083,
	 author = {Simone  Fior and Mingai  Li and Bengt  Oxelman and Roberto  Viola and Scott A. Hodges and Lino  Ometto and Claudio  Varotto},
	 title = {Spatiotemporal reconstruction of the Aquilegia rapid radiation through next-generation sequencing of rapidly evolving cpDNA regions.},
	 year = {2013},
	 keywords = {Aquilegia, Next-generation sequencing, Ranunculaceae molecular dat- ing, rapidly evolving noncoding cpDNA, recent rapid radiation, Roche 454.},
	 doi = {},
	 url = {http://},
	 pmid = {},
	 journal = {New Phytologist},
	 volume = {198},
	 number = {},
	 pages = {579--592},
	 abstract = {Aquilegia is a well-known model system in the field of evolutionary biology, but obtaining a resolved and well-supported phylogenetic reconstruction for the genus has been hindered by its recent and rapid diversification.  Here, we applied 454 next-generation sequencing to PCR amplicons of 21 of the most rap- idly evolving regions of the plastome to generate c. 24 kb of sequences from each of 84 indi- viduals from throughout the genus.  The resulting phylogeny has well-supported resolution of the main lineages of the genus, although recent diversification such as in the European taxa remains unresolved. By producing a chronogram of the whole Ranunculaceae family based on published data, we inferred cali- bration points for dating the Aquilegia radiation. The genus originated in the upper Miocene c. 6.9 million yr ago (Ma) in Eastern Asia, and diversification occurred c. 4.8 Ma with the split of two main clades, one colonizing North America, and the other Western Eurasia through the mountains of Central Asia. This was followed by a back-to-Asia migration, originating from the European stock using a North Asian route.  These results provide the first backbone phylogeny and spatiotemporal reconstruction of the Aquilegia radiation, and constitute a robust framework to address the adaptative nature of speciation within the group.}
}

Show RIS reference

TY - JOUR
ID - 22083
AU - Fior,Simone
AU - Li,Mingai
AU - Oxelman,Bengt
AU - Viola,Roberto
AU - Hodges,Scott A.
AU - Ometto,Lino
AU - Varotto,Claudio
T1 - Spatiotemporal reconstruction of the Aquilegia rapid radiation through next-generation sequencing of rapidly evolving cpDNA regions.
PY - 2013
KW - Aquilegia
KW - Next-generation sequencing
KW - Ranunculaceae molecular dat- ing
KW - rapidly evolving noncoding cpDNA
KW - recent rapid radiation
KW - Roche 454.
UR - http://dx.doi.org/
N2 - Aquilegia is a well-known model system in the field of evolutionary biology, but obtaining a resolved and well-supported phylogenetic reconstruction for the genus has been hindered by its recent and rapid diversification. Here, we applied 454 next-generation sequencing to PCR amplicons of 21 of the most rap- idly evolving regions of the plastome to generate c. 24 kb of sequences from each of 84 indi- viduals from throughout the genus. The resulting phylogeny has well-supported resolution of the main lineages of the genus, although recent diversification such as in the European taxa remains unresolved. By producing a chronogram of the whole Ranunculaceae family based on published data, we inferred cali- bration points for dating the Aquilegia radiation. The genus originated in the upper Miocene c. 6.9 million yr ago (Ma) in Eastern Asia, and diversification occurred c. 4.8 Ma with the split of two main clades, one colonizing North America, and the other Western Eurasia through the mountains of Central Asia. This was followed by a back-to-Asia migration, originating from the European stock using a North Asian route. These results provide the first backbone phylogeny and spatiotemporal reconstruction of the Aquilegia radiation, and constitute a robust framework to address the adaptative nature of speciation within the group.
L3 -
JF - New Phytologist
VL - 198
IS -
SP - 579
EP - 592
ER -