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Citation for Study 15322

Citation title: "A taxonomic study of snow Chloromonas species (Volvocales, Chlorophyceae) based on light and electron microscopy and molecular analysis of cultured material".

Study name: "A taxonomic study of snow Chloromonas species (Volvocales, Chlorophyceae) based on light and electron microscopy and molecular analysis of cultured material".

This study is part of submission 15322 (Status: Published).

Citation

Matsuzaki R., Hara Y., & Nozaki H. 2014. A taxonomic study of snow Chloromonas species (Volvocales, Chlorophyceae) based on light and electron microscopy and molecular analysis of cultured material. Phycologia, 53(3): 293-304.

Authors

• Matsuzaki R. (submitter) +81-29-850-2204
• Hara Y.
• Nozaki H. +81-3-5841-4048

Abstract

Although zygote morphology is important in traditional taxonomic systems of snow-inhabiting species of Chloromonas (Volvocales, Chlorophyceae), induction of sexual reproduction or hypnospore formation is difficult in culture. Thus, alternative taxonomic methodologies are required when sexual reproduction does not occur in cultures. Here, we performed a taxonomic study of snow-inhabiting Chloromonas species based on culture strains with elongate or ellipsoidal vegetative cells. Our comparative light and electron microscopy demonstrated that the strains were clearly distinguished into six species based on differences in vegetative cell shape and chloroplast morphology, the number of zoospores within the parental cell and the presence or absence of cell aggregates in old cultures. The six species are Chloromonas chenangoensis, C. fukushimae sp. nov., C. hohamii, C. pichinchae, C. tenuis sp. nov. and C. tughillensis. Although four of the six species formed a small clade based on phylogenetic analysis of multiple genes, their separation was supported by comparison of secondary structures of the internal transcribed spacer 2 of nuclear ribosomal DNA and genetic differences of nuclear- and chloroplast-encoded genes. The present study demonstrated that polyphasic analyses of culture strains of snow Chloromonas species could be used for the identification of natural species.

Keywords

Chloromonadinia; Chloromonas fukushimae; Chloromonas tenuis; Molecular phylogeny; Morphology; Snow algae; Taxonomy; Ultrastructure

External links

• DOI: 10.2216/14-3.1
• Other URL: http://www.phycologia.org/doi/abs/10.2216/14-3.1

About this resource

• Canonical resource URI: http://purl.org/phylo/treebase/phylows/study/TB2:S15322
• Other versions: Nexus NeXML
• Show BibTeX reference

  		@ARTICLE{TreeBASE2Ref22822,
  	 author = {Ryo  Matsuzaki and Yoshiaki  Hara and Hisayoshi  Nozaki},
  	 title = {A taxonomic study of snow Chloromonas species (Volvocales, Chlorophyceae) based on light and electron microscopy and molecular analysis of cultured material},
  	 year = {2014},
  	 keywords = {Chloromonadinia; Chloromonas fukushimae; Chloromonas tenuis; Molecular phylogeny; Morphology; Snow algae; Taxonomy; Ultrastructure},
  	 doi = {10.2216/14-3.1},
  	 url = {http://www.phycologia.org/doi/abs/10.2216/14-3.1},
  	 pmid = {},
  	 journal = {Phycologia},
  	 volume = {53},
  	 number = {3},
  	 pages = {293--304},
  	 abstract = {Although zygote morphology is important in traditional taxonomic systems of snow-inhabiting species of Chloromonas (Volvocales, Chlorophyceae), induction of sexual reproduction or hypnospore formation is difficult in culture. Thus, alternative taxonomic methodologies are required when sexual reproduction does not occur in cultures. Here, we performed a taxonomic study of snow-inhabiting Chloromonas species based on culture strains with elongate or ellipsoidal vegetative cells. Our comparative light and electron microscopy demonstrated that the strains were clearly distinguished into six species based on differences in vegetative cell shape and chloroplast morphology, the number of zoospores within the parental cell and the presence or absence of cell aggregates in old cultures. The six species are Chloromonas chenangoensis, C. fukushimae sp. nov., C. hohamii, C. pichinchae, C. tenuis sp. nov. and C. tughillensis. Although four of the six species formed a small clade based on phylogenetic analysis of multiple genes, their separation was supported by comparison of secondary structures of the internal transcribed spacer 2 of nuclear ribosomal DNA and genetic differences of nuclear- and chloroplast-encoded genes. The present study demonstrated that polyphasic analyses of culture strains of snow Chloromonas species could be used for the identification of natural species.}
  }
  		

• Show RIS reference

  		TY  - JOUR
  ID  - 22822
  AU  - Matsuzaki,Ryo 
  AU  - Hara,Yoshiaki 
  AU  - Nozaki,Hisayoshi 
  T1  - A taxonomic study of snow Chloromonas species (Volvocales, Chlorophyceae) based on light and electron microscopy and molecular analysis of cultured material
  PY  - 2014
  KW  - Chloromonadinia; Chloromonas fukushimae; Chloromonas tenuis; Molecular phylogeny; Morphology; Snow algae; Taxonomy; Ultrastructure
  UR  - http://www.phycologia.org/doi/abs/10.2216/14-3.1
  N2  - Although zygote morphology is important in traditional taxonomic systems of snow-inhabiting species of Chloromonas (Volvocales, Chlorophyceae), induction of sexual reproduction or hypnospore formation is difficult in culture. Thus, alternative taxonomic methodologies are required when sexual reproduction does not occur in cultures. Here, we performed a taxonomic study of snow-inhabiting Chloromonas species based on culture strains with elongate or ellipsoidal vegetative cells. Our comparative light and electron microscopy demonstrated that the strains were clearly distinguished into six species based on differences in vegetative cell shape and chloroplast morphology, the number of zoospores within the parental cell and the presence or absence of cell aggregates in old cultures. The six species are Chloromonas chenangoensis, C. fukushimae sp. nov., C. hohamii, C. pichinchae, C. tenuis sp. nov. and C. tughillensis. Although four of the six species formed a small clade based on phylogenetic analysis of multiple genes, their separation was supported by comparison of secondary structures of the internal transcribed spacer 2 of nuclear ribosomal DNA and genetic differences of nuclear- and chloroplast-encoded genes. The present study demonstrated that polyphasic analyses of culture strains of snow Chloromonas species could be used for the identification of natural species.
  L3  - 10.2216/14-3.1
  JF  - Phycologia
  VL  - 53
  IS  - 3
  SP  - 293
  EP  - 304
  ER  -