@ARTICLE{TreeBASE2Ref20549,
author = {Jeremy Catalin Andersen and Nicholas J Mills},
title = {DNA extraction from museum specimens of parasitic Hymenoptera},
year = {2012},
keywords = {ancient DNA, Insect Museum, Braconidae, Trioxys, Metoerus, Atanycolus},
doi = {10.1371/journal.pone.0045549},
url = {http://},
pmid = {},
journal = {PLoS ONE},
volume = {7},
number = {10},
pages = {e45549},
abstract = {At the same time that molecular researchers are improving techniques to extract DNA from museum specimens, this increased demand for access to museum specimens has created tension between the need to preserve specimens for maintaining collections and morphological research and the desire to conduct molecular analyses. To address these concerns, we examined the suitability of non-invasive DNA extraction techniques on three species of parasitic Hymenoptera (Braconidae), and test the effects of body size (parasitoid species), age (time since collection), and DNA concentration from each extract on the probability of amplifying meaningful fragments of two commonly used genetic loci. We found that age was a significant factor for determining the probability of success for sequencing both 28S and COI fragments. While the size of the braconid parasitoids significantly affected the total amount of extracted DNA, neither size nor DNA concentration were significant factors for the amplification of either gene region. We also tested several primer combinations of various lengths, but were unable to amplify fragments longer than ,150 base pairs. These short fragments of 28S and COI were however sufficient for species identification, and for the discovery of within species genetic variation.}
}
Citation for Study 12519
Citation title:
"DNA extraction from museum specimens of parasitic Hymenoptera".
Study name:
"DNA extraction from museum specimens of parasitic Hymenoptera".
This study is part of submission 12519
(Status: Published).
Citation
Andersen J.C., & Mills N.J. 2012. DNA extraction from museum specimens of parasitic Hymenoptera. PLoS ONE, 7(10): e45549.
Authors
-
Andersen J.C.
(submitter)
-
Mills N.J.
Abstract
At the same time that molecular researchers are improving techniques to extract DNA from museum specimens, this increased demand for access to museum specimens has created tension between the need to preserve specimens for maintaining collections and morphological research and the desire to conduct molecular analyses. To address these concerns, we examined the suitability of non-invasive DNA extraction techniques on three species of parasitic Hymenoptera (Braconidae), and test the effects of body size (parasitoid species), age (time since collection), and DNA concentration from each extract on the probability of amplifying meaningful fragments of two commonly used genetic loci. We found that age was a significant factor for determining the probability of success for sequencing both 28S and COI fragments. While the size of the braconid parasitoids significantly affected the total amount of extracted DNA, neither size nor DNA concentration were significant factors for the amplification of either gene region. We also tested several primer combinations of various lengths, but were unable to amplify fragments longer than ,150 base pairs. These short fragments of 28S and COI were however sufficient for species identification, and for the discovery of within species genetic variation.
Keywords
ancient DNA, Insect Museum, Braconidae, Trioxys, Metoerus, Atanycolus
External links
About this resource
- Canonical resource URI:
http://purl.org/phylo/treebase/phylows/study/TB2:S12519
- Other versions:
Nexus
NeXML
- Show BibTeX reference
@ARTICLE{TreeBASE2Ref20549,
author = {Jeremy Catalin Andersen and Nicholas J Mills},
title = {DNA extraction from museum specimens of parasitic Hymenoptera},
year = {2012},
keywords = {ancient DNA, Insect Museum, Braconidae, Trioxys, Metoerus, Atanycolus},
doi = {10.1371/journal.pone.0045549},
url = {http://},
pmid = {},
journal = {PLoS ONE},
volume = {7},
number = {10},
pages = {e45549},
abstract = {At the same time that molecular researchers are improving techniques to extract DNA from museum specimens, this increased demand for access to museum specimens has created tension between the need to preserve specimens for maintaining collections and morphological research and the desire to conduct molecular analyses. To address these concerns, we examined the suitability of non-invasive DNA extraction techniques on three species of parasitic Hymenoptera (Braconidae), and test the effects of body size (parasitoid species), age (time since collection), and DNA concentration from each extract on the probability of amplifying meaningful fragments of two commonly used genetic loci. We found that age was a significant factor for determining the probability of success for sequencing both 28S and COI fragments. While the size of the braconid parasitoids significantly affected the total amount of extracted DNA, neither size nor DNA concentration were significant factors for the amplification of either gene region. We also tested several primer combinations of various lengths, but were unable to amplify fragments longer than ,150 base pairs. These short fragments of 28S and COI were however sufficient for species identification, and for the discovery of within species genetic variation.}
}
- Show RIS reference
TY - JOUR
ID - 20549
AU - Andersen,Jeremy Catalin
AU - Mills,Nicholas J
T1 - DNA extraction from museum specimens of parasitic Hymenoptera
PY - 2012
KW - ancient DNA
KW - Insect Museum
KW - Braconidae
KW - Trioxys
KW - Metoerus
KW - Atanycolus
UR - http://dx.doi.org/10.1371/journal.pone.0045549
N2 - At the same time that molecular researchers are improving techniques to extract DNA from museum specimens, this increased demand for access to museum specimens has created tension between the need to preserve specimens for maintaining collections and morphological research and the desire to conduct molecular analyses. To address these concerns, we examined the suitability of non-invasive DNA extraction techniques on three species of parasitic Hymenoptera (Braconidae), and test the effects of body size (parasitoid species), age (time since collection), and DNA concentration from each extract on the probability of amplifying meaningful fragments of two commonly used genetic loci. We found that age was a significant factor for determining the probability of success for sequencing both 28S and COI fragments. While the size of the braconid parasitoids significantly affected the total amount of extracted DNA, neither size nor DNA concentration were significant factors for the amplification of either gene region. We also tested several primer combinations of various lengths, but were unable to amplify fragments longer than ,150 base pairs. These short fragments of 28S and COI were however sufficient for species identification, and for the discovery of within species genetic variation.
L3 - 10.1371/journal.pone.0045549
JF - PLoS ONE
VL - 7
IS - 10
ER -
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