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Citation for Study 27596

Citation title: "Evaluating species in Botryosphaeriales".

Study name: "Evaluating species in Botryosphaeriales".

This study is part of submission 27596 (Status: Published).

Citation

Zhang W., Groenewald J.Z., Lombard L., Schumacher R.K., Phillips A., & Crous P.W. 2021. Evaluating species in Botryosphaeriales. Persoonia, 46: 63-115.

Authors

• Zhang W. 1389362509
• Groenewald J.Z. (submitter) +31302122600
• Lombard L.
• Schumacher R.K.
• Phillips A.
• Crous P.W.

Abstract

The Botryosphaeriales (Dothideomycetes) includes numerous endophytic, saprobic, and plant pathogenic species associated with a wide range of symptoms, most commonly on woody plants. In a recent phylogenetic treatment of 499 isolates in the culture collection (CBS) of the Westerdijk Institute, we evaluated the families and genera accommodated in this order of important fungi. The present study presents multigene phylogenetic analyses for an additional 230 isolates, using ITS, tef1, tub2, LSU and rpb2 loci, in combination with morphological data. Based on these data, 58 species are reduced to synonymy, and eight novel species are described. They include Diplodia afrocarpi (Afrocarpus, South Africa), Dothiorella diospyricola (Diospyros, South Africa), Lasiodiplodia acaciae (Acacia, Indonesia), Neofusicoccum podocarpi (Podocarpus, South Africa), N. rapaneae (Rapanea, South Africa), Phaeobotryon ulmi (Ulmus, Germany), Saccharata grevilleae (Grevillea, Australia) and S. hakeiphila (Hakea, Australia). The results have clarified the identity of numerous isolates that lacked Latin binomials or had been deposited under incorrect names in the CBS collection in the past. They also provide a solid foundation for more in-depth future studies on taxa in the order. Sequences of the tef1, tub2 and rpb2 genes proved to be the most reliable markers. At the species level, results showed that the most informative genes were inconsistent, but that a combination of four candidate barcodes (ITS, tef1, tub2 and rpb2) provided reliable resolution. Furthermore, given the large number of additional isolates included in this study, and newly generated multigene DNA datasets, several species could also be reduced to synonymy. The study illustrates the value of reassessing the identity of older collections in culture collections utilising modern taxonomic frameworks and methods.

Keywords

Multi-Locus Sequence Typing (MLST); canker and leaf spot pathogens; new taxa; systematics

External links

• DOI: 10.3767/persoonia.2021.46.03
• Other URL: http://doi.org/10.3767/persoonia.2021.46.03

About this resource

• Canonical resource URI: http://purl.org/phylo/treebase/phylows/study/TB2:S27596
• Other versions: Nexus NeXML
• Show BibTeX reference

  		@ARTICLE{TreeBASE2Ref31676,
  	 author = {Wu  Zhang and  Johannes (Ewald) Zacharias Groenewald and Lorenzo  Lombard and Rene K. Schumacher and Alan John Lander  Phillips and Pedro W. Crous},
  	 title = {Evaluating species in Botryosphaeriales},
  	 year = {2021},
  	 keywords = {Multi-Locus Sequence Typing (MLST); canker and leaf spot pathogens; new taxa; systematics},
  	 doi = {10.3767/persoonia.2021.46.03},
  	 url = {http://doi.org/10.3767/persoonia.2021.46.03},
  	 pmid = {},
  	 journal = {Persoonia},
  	 volume = {46},
  	 number = {},
  	 pages = {63--115},
  	 abstract = {The Botryosphaeriales (Dothideomycetes) includes numerous endophytic, saprobic, and plant pathogenic species associated with a wide range of symptoms, most commonly on woody plants. In a recent phylogenetic treatment of 499 isolates in the culture collection (CBS) of the Westerdijk Institute, we evaluated the families and genera accommodated in this order of important fungi. The present study presents multigene phylogenetic analyses for an additional 230 isolates, using ITS, tef1, tub2, LSU and rpb2 loci, in combination with morphological data. Based on these data, 58 species are reduced to synonymy, and eight novel species are described. They include Diplodia afrocarpi (Afrocarpus, South Africa), Dothiorella diospyricola (Diospyros, South Africa), Lasiodiplodia acaciae (Acacia, Indonesia), Neofusicoccum podocarpi (Podocarpus, South Africa), N. rapaneae (Rapanea, South Africa), Phaeobotryon ulmi (Ulmus, Germany), Saccharata grevilleae (Grevillea, Australia) and S. hakeiphila (Hakea, Australia). The results have clarified the identity of numerous isolates that lacked Latin binomials or had been deposited under incorrect names in the CBS collection in the past. They also provide a solid foundation for more in-depth future studies on taxa in the order. Sequences of the tef1, tub2 and rpb2 genes proved to be the most reliable markers. At the species level, results showed that the most informative genes were inconsistent, but that a combination of four candidate barcodes (ITS, tef1, tub2 and rpb2) provided reliable resolution. Furthermore, given the large number of additional isolates included in this study, and newly generated multigene DNA datasets, several species could also be reduced to synonymy. The study illustrates the value of reassessing the identity of older collections in culture collections utilising modern taxonomic frameworks and methods.}
  }
  		

• Show RIS reference

  		TY  - JOUR
  ID  - 31676
  AU  - Zhang,Wu 
  AU  - Groenewald, Johannes (Ewald) Zacharias
  AU  - Lombard,Lorenzo 
  AU  - Schumacher,Rene K.
  AU  - Phillips,Alan John Lander 
  AU  - Crous,Pedro W.
  T1  - Evaluating species in Botryosphaeriales
  PY  - 2021
  KW  - Multi-Locus Sequence Typing (MLST); canker and leaf spot pathogens; new taxa; systematics
  UR  - http://doi.org/10.3767/persoonia.2021.46.03
  N2  - The Botryosphaeriales (Dothideomycetes) includes numerous endophytic, saprobic, and plant pathogenic species associated with a wide range of symptoms, most commonly on woody plants. In a recent phylogenetic treatment of 499 isolates in the culture collection (CBS) of the Westerdijk Institute, we evaluated the families and genera accommodated in this order of important fungi. The present study presents multigene phylogenetic analyses for an additional 230 isolates, using ITS, tef1, tub2, LSU and rpb2 loci, in combination with morphological data. Based on these data, 58 species are reduced to synonymy, and eight novel species are described. They include Diplodia afrocarpi (Afrocarpus, South Africa), Dothiorella diospyricola (Diospyros, South Africa), Lasiodiplodia acaciae (Acacia, Indonesia), Neofusicoccum podocarpi (Podocarpus, South Africa), N. rapaneae (Rapanea, South Africa), Phaeobotryon ulmi (Ulmus, Germany), Saccharata grevilleae (Grevillea, Australia) and S. hakeiphila (Hakea, Australia). The results have clarified the identity of numerous isolates that lacked Latin binomials or had been deposited under incorrect names in the CBS collection in the past. They also provide a solid foundation for more in-depth future studies on taxa in the order. Sequences of the tef1, tub2 and rpb2 genes proved to be the most reliable markers. At the species level, results showed that the most informative genes were inconsistent, but that a combination of four candidate barcodes (ITS, tef1, tub2 and rpb2) provided reliable resolution. Furthermore, given the large number of additional isolates included in this study, and newly generated multigene DNA datasets, several species could also be reduced to synonymy. The study illustrates the value of reassessing the identity of older collections in culture collections utilising modern taxonomic frameworks and methods.
  L3  - 10.3767/persoonia.2021.46.03
  JF  - Persoonia
  VL  - 46
  IS  - 
  SP  - 63
  EP  - 115
  ER  -