@ARTICLE{TreeBASE2Ref17454, author = {Andrew M. Schurko and Leonel Mendoza and C. A. Levesque and N. L. Desaulniers and Arthur W. A. M. de Cock and Glen R. Klassen}, title = {A molecular phylogeny of Pythium insidiosum.}, year = {2003}, keywords = {}, doi = {}, url = {}, pmid = {}, journal = {Molecular Ecology}, volume = {}, number = {}, pages = {}, abstract = {Sequence analysis of the ribosomal DNA internal transcribed spacers (ITS) was used to establish phylogenetic relationships among 23 isolates of Pythium insidiosum, the etiological agent of pythiosis in mammals. The isolates were divided into three distinct clades which exhibited significant geographic isolation. Clade I consisted of isolates from North and South America while clade II contained isolates from Asia and Australia. Also present in clade II was an isolate from a patient in United States, but the origin of infection may in fact be in the Middle East. Clade III was comprised of isolates from Thailand and the United States. All 23 P. insidiosum isolates were more closely related to each other than to any other Pythium species in this study. Additionally, all Pythium isolates formed a clade separate from both outgroup species, Phytophthora megasperma and Lagenidium giganteum. The ITS sequence results tend to support evidence for geographic variants within P. insidiosum or that P. insidiosum is in fact comprised of more than one species. As well, the sequence information obtained provides an abundance of molecular data for applications in the diagnosis of pythiosis and identification of P. insidiosum from clinical samples.} }

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