@ARTICLE{TreeBASE2Ref21605, author = {Wataru Shinohara}, title = {The use of matK in Ophioglossaceae Phylogeny and the determination of Mankyua chromosome number}, year = {2012}, keywords = {Chromosome number, Helminthostachys, Mankyua chejuense, matK, Ophioglossaceae}, doi = {}, url = {http://}, pmid = {}, journal = {Systematic Botany}, volume = {}, number = {}, pages = {}, abstract = {The chromosome number and phylogenetic position of the monotypic eusporangiate genus Mankyua (Ophioglossaceae), which shares peculiar morphological characteristics with Ophioglossum s. l. and Helminthostachys, were determined. Meiotic and mitotic chromosome observations of Mankyua show n = 130 and 2n = ca. 260, suggesting that the base number of this genus is x = 130, which is a new finding and the highest base number reported so far for Ophioglossaceae. The molecular phylogenetic trees derived from nucleotide sequence variation of rbcL and matK genes differ in terms of the phylogenetic position of Mankyua. The molecular phylogenetic trees based on matK, which show higher bootstrap values or posterior probabilities than those of rbcL, suggest that Mankyua might be the sister group of the clade comprised of Helminthostachys and Ophioglossum s. l.} }

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