@ARTICLE{TreeBASE2Ref19101, author = {Torbjorn Ekrem and Elisabeth Stur and Paul D. N. Hebert}, title = {Females do count: Documenting Chironomidae (Diptera) species diversity using DNA barcoding}, year = {2010}, keywords = {DNA barcoding, biodiversity, insects, freshwater, Diptera, Chironomidae}, doi = {10.1007/s13127-010-0034-y}, url = {http://}, pmid = {}, journal = {Organisms Diversity and Evolution}, volume = {10}, number = {}, pages = {397--408}, abstract = {Because the family Chironomidae, or non-biting midges, is one of the most species rich groups of macroinvertebrates in freshwater, species-level identifications of chironomids are important for biodiversity assessments in these ecosystems. We used DNA barcoding to investigate how inclusion of the often neglected adult females would influence the species count from springs and spring brooks at S?lendet Nature Reserve in Central Norway. Using DNA barcodes we were able to associate 77.6% of the females with males from the study site or other regions while the remainder were unlinked and could therefore only be identified to genus level. Our results show that the number of recorded species increased by 27% when females were included. We also found that DNA barcoding is effective for the detection of taxonomically challenging species and species groups. Using DNA barcoding in combination with traditional taxonomy, we recognized at least five species new to science and three species and one genus new to Norway.} }

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