@ARTICLE{TreeBASE2Ref19335, author = {John D Hall and Karolina Fucikova and Chien Lo and Louise A. Lewis and Kenneth G. Karol}, title = {An assessment of proposed DNA barcodes in freshwater green algae.}, year = {2010}, keywords = {DNA barcode, green algae, Charophyceae, Chlorophyceae, Zygnematophyceae, 18S rDNA, cox1, ITS rDNA, rbcL, tufA, UPA}, doi = {}, url = {http://}, pmid = {}, journal = {Cryptogamie Algologie}, volume = {31}, number = {4}, pages = {}, abstract = {The development of DNA barcodes for green algae would aid in their accurate and rapid identification. This is particularly important for the green algae because in many genera structural characters are few or difficult to observe. In this study we test the utility of several DNA barcode loci in seven distantly related species groups of charophytes and chlorophytes. The ITS rDNA and cox1 markers were the most variable, although cox1 was least applicable because it was successfully amplified and sequenced in only one test genus. The ITS1 and ITS2 rDNA regions each amplified only a subset of the charophytes. Both rbcL and tufA were moderately variable, although tufA may pose challenges in some lineages where the gene is encoded in the nucleus. The 18S rDNA and UPA were the least variable loci tested. Of the markers tested, rbcL, ITS and tufA (in chlorophytes) are the most promising for use as DNA barcodes. However, none of the loci tested were ideal for use across all tested lineages of green algae because of taxon-specific applicability and because none of the markers could distinguish closely related species in all groups tested.} }

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