@ARTICLE{TreeBASE2Ref15668, author = {M. L. Guillemin and S. A. Akki and Thierry Givernaud and Aziza Mouradi and Myriam Valero and Christophe Destombe}, title = {Molecular characterisation and development of rapid molecular methods to identify species of Gracilariaceae from the Atlantic coast of Morocco}, year = {2008}, keywords = {}, doi = {10.1016/j.aquabot.2008.03.008}, url = {}, pmid = {}, journal = {Aquatic Botany}, volume = {}, number = {}, pages = {}, abstract = {In Gracilariaceae, species identification is traditionally based on gross morphology; therefore taxonomic status of terete individuals remains frequently problematic due to the high morphological plasticity. Different morphological species have been recorded along the Atlantic coast of Morocco; however, no clear diagnostic characters were available to discriminate between cylindrical species. In this study, molecular markers were used to characterize species. The sequence of the Rubisco spacer allowed identification of six species of Gracilariaceae: G. gracilis, G. dura, G. conferta, G. vermiculophylla and G. multipartita and Gracilariopsis longissima. In order to identify species with certainty, two simple and rapid methods based on the amplification of rDNA ITS and PCR-RFPL of the large subunit of the Rubisco were developed.} }

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