@ARTICLE{TreeBASE2Ref17680, author = {Claire Streten and Karen S Gibb}, title = {Genetic variation in Candidatus Phytoplasma}, year = {2004}, keywords = {}, doi = {}, url = {}, pmid = {}, journal = {Plant Pathology}, volume = {}, number = {}, pages = {}, abstract = {Diversity studies of phytoplasmas have shown that the conserved 16S rRNA gene may not distinguish closely related phytoplasmas such as members of Candidatus Phytoplasma australiense. This study examined whether genes that are less conserved than the 16S rRNA gene would distinguish Ca. P. australiense strains that are identical based on their 16S rRNA genes. The capacity to distinguish these phytoplasmas may provide insight into their origin and distribution, and may show a pattern of association with particular plant hosts. Sequence analysis of the tuf gene and rp operon showed that Ca. P. australiense strains could be differentiated into four subgroups, which were named 16SrXII-B(tuf-Australia I)(rp-A), 16SrXII-B(tuf-New Zealand I; rp-B), 16SrXII-B(tuf-New Zealand II) and 16SrXII-B (rp-C). Strawberry lethal yellows 1 (SLY1), strawberry green petal (SGP), Australian grapevine yellows, pumpkin yellow leaf curl and cottonbush witches broom phytoplasmas were designated members of 16SrXII-B (tuf-Australia I; rp-A) subgroup. The strawberry lethal yellows 2 and cottonbush reduced yellow leaves phytoplasmas were assigned to 16SrXII (tuf-New Zealand II; rp-B) subgroup. No relationship was observed between these phytoplasma subgroups and collection date, location or host plant. However, the study revealed evolutionary divergence in the 16SrXII group.} }

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