@ARTICLE{TreeBASE2Ref21597, author = {Tetyana Tsykun and Daniel Rigling and Simone Prospero}, title = {A new multilocus approach for a reliable DNA-based identification of Armillaria species.}, year = {2013}, keywords = {}, doi = {}, url = {http://}, pmid = {}, journal = {Mycologia}, volume = {}, number = {}, pages = {}, abstract = {During the past two decades, morphology-based identification of fungal species has gradually been replaced by DNA-based identification. In this paper, we highlight and critically discuss possible limitations of the molecular methods via the example of the basidiomycete genus Armillaria. We analyzed a total of 144 sequences of three DNA regions commonly used for identifying fungi (ribosomal IGS-1 and ITS regions; translation elongation factor-1 alpha gene) from 48 specimens of six Armillaria species occurring in Europe (A. cepistipes, A. ostoyae, A. gallica, A. borealis, A. mellea, and A. tabescens). Species were identified by comparing our sequences with those from the NCBI database, phylogenetic analyses, and PCR-RFLP analyses of the three regions considered. When analyzed separately, no region could unambiguously identify all six Armillaria species because of low interspecific and high intra-sequence variability. We therefore developed a multilocus approach, which involves the stepwise use of the three regions. Following this scheme, all six species could be clearly discriminated. Our study suggests that, to improve the reliability of DNA-based techniques for species identification, multiple genes or intergenic regions should be analyzed.} }

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