@ARTICLE{TreeBASE2Ref17058, author = {Stephen W. Peterson and Fernando E. Vega and Francisco Posada and Chifumi Nagai}, title = {Penicillium coffeae a new endophytic species isolated from a coffee plant and its phylogenetic relationship to P. fellutanum, P. thiersii and P. brocae based on parsimony analysis of multilocus DNA sequences}, year = {2005}, keywords = {}, doi = {}, url = {}, pmid = {}, journal = {Mycologia}, volume = {}, number = {}, pages = {}, abstract = {Penicillium coffeae is described as a novel endophyte isolated from a Coffeae arabica L. plant in Hawaii. The species is slow growing with short, vesiculate, monoverticillate conidiophores. Phylogenetic analysis using three loci shows that P. coffeae forms a strongly supported clade with P. fellutanum, P. charlesii, P. chermesinum, P. indicum, P. phoeniceum and P. brocae. Phenotypic analysis shows that these species are quite similar but can be distinguished. The EF-1a gene from P. fellutanum, P. charlesii, P. chermesinum and P. indicum lack introns, P. coffeae and P. phoeniceum have a previously unknown intron at codon 20 and P. brocae and P. thiersii isolates have a single intron at codon 26. The most parsimonious interpretation of the strongly supported phylogenetic tree requires the gain of a novel intron at position 20 and loss of intron 26 to arrive at the current distribution of introns in this gene. This is one of only a few examples of intron gain in genes.} }

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