@ARTICLE{TreeBASE2Ref29956, author = {Pei Tian and Wenbo Xu and Chunjie Li and Hui Song and Meining Wang and Christopher Lewis Schardl and Zhibiao Nan}, title = {Confirmation and taxonomy of one hybrid Epichlo? species symbiotic with Festuca sinensis in Northwest of China.}, year = {2019}, keywords = {Epichlo?, endophyte; Festuca sinensis; hybrid species; morphology; phylogenetic relationships }, doi = {}, url = {http://}, pmid = {}, journal = {Mycologia}, volume = {}, number = {}, pages = {}, abstract = {More than forty unique taxa in Epichlo? that form associations with grasses in the sub family Pooideae have been described based on morphology, host specificity and phylogenetic analyses. In this study, a new Epichlo? species, Epichlo? sinensis was isolated from seeds of endophyte-infected Festuca sinensis that were collected from northwest China. This endophyte had typical morphological characteristics of the Epichlo? genus. Endophyte hyphae were long, thin, septate, a little curved and sparsely branched in seed aleurone layer and were orientated longitudinally and parallel to each other without branching in the intercellular spaces of leaf sheaths of plants grown from seeds. In culture of PDA, endophyte colonies are smooth, cottony and white. Phylogenetic relationships showed that this endophyte was an asexual interspecific hybrid of the E. typhina complex (ETC, E. typhina subsp. poae ) and E. sibirica clades based on introns sequences of the housekeeping genes β-tubulin (tubB) and translation elongation factor 1-α (tefA). Morphological and phylogenetic evidence supported the designation of isolates from F. sinensis as a new interspecific hybrid species. } }

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