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Citation for Study 14849

Citation title: "Midgut Transcriptome Profiling of Anoplophora glabripennis, a Lignocellulose Degrading Cerambycid Beetle".

Study name: "Midgut Transcriptome Profiling of Anoplophora glabripennis, a Lignocellulose Degrading Cerambycid Beetle".

This study is part of submission 14849 (Status: Published).

Citation

Scully E.D., Hoover K., Carlson J.E., Tien M., & Geib S.M. 2013. Midgut Transcriptome Profiling of Anoplophora glabripennis, a Lignocellulose Degrading Cerambycid Beetle. BMC Genomics, 14(850).

Authors

• Scully E.D. (submitter) 814-873-5399
• Hoover K.
• Carlson J.E.
• Tien M.
• Geib S.M.

Abstract

Wood-feeding insects often work in collaboration with microbial symbionts to degrade lignin biopolymers and release glucose and other fermentable sugars from recalcitrant plant cell wall carbohydrates, including cellulose and hemicellulose. Here, we present the midgut transcriptome of larval Anoplophora glabripennis, a wood-boring beetle with documented lignin-, cellulose-, and hemicellulose- degrading capabilities, which provides valuable insights into how this insect overcomes challenges associated with feeding in woody tissue. Transcripts from putative protein coding regions of over 9,000 insect-derived genes were identified in the A. glabripennis midgut transcriptome using a combination of 454 shotgun and Illumina paired-end reads. The most highly-expressed genes predicted to encode digestive-related enzymes were trypsins, carboxylesterases, β-glucosidases, and cytochrome P450s. Furthermore, 180 unigenes predicted to encode glycoside hydrolases (GHs) were identified and included several GH 5, 45, and 48 cellulases, GH 1 xylanases, and GH 1 β-glucosidases. In addition, transcripts predicted to encode enzymes involved in detoxification were detected, including a substantial number of unigenes classified as cytochrome P450s (CYP6B) and carboxylesterases, which are hypothesized to play pivotal roles in detoxifying host tree defensive chemicals and could make important contributions to A. glabripennis? expansive host range. While a large diversity of insect-derived transcripts predicted to encode digestive and detoxification enzymes were detected, few transcripts predicted to encode enzymes required for lignin degradation or synthesis of essential nutrients were identified, suggesting that collaboration with microbial enzymes may be required for survival in woody tissue. A. glabripennis produces a number of enzymes with putative roles in cell wall digestion, detoxification, and nutrient extraction, which likely contribute to its ability to thrive in a broad range of host trees. This system is quite different from the previously characterized termite fermentation system and provides new opportunities to discover enzymes that could be exploited for cellulosic ethanol biofuel production or the development of novel methods to control wood-boring pests.

Keywords

cellulase, carboxylesterase, cytochrome P450, xylanase, comparative transcriptomics, hemicellulose, biofuels; beta-glucosidase

External links

• DOI: 10.1186/1471-2164-14-850
• Other URL: http://www.biomedcentral.com/1471-2164/14/850

About this resource

• Canonical resource URI: http://purl.org/phylo/treebase/phylows/study/TB2:S14849
• Other versions: Nexus NeXML
• Show BibTeX reference

  		@ARTICLE{TreeBASE2Ref22500,
  	 author = {Erin D Scully and Kelli  Hoover and John E Carlson and Ming  Tien and Scott M Geib},
  	 title = {Midgut Transcriptome Profiling of Anoplophora glabripennis, a Lignocellulose Degrading Cerambycid Beetle},
  	 year = {2013},
  	 keywords = {cellulase, carboxylesterase, cytochrome P450, xylanase, comparative transcriptomics, hemicellulose, biofuels; beta-glucosidase},
  	 doi = {10.1186/1471-2164-14-850},
  	 url = {http://www.biomedcentral.com/1471-2164/14/850},
  	 pmid = {},
  	 journal = {BMC Genomics},
  	 volume = {14},
  	 number = {850},
  	 pages = {},
  	 abstract = {Wood-feeding insects often work in collaboration with microbial symbionts to degrade lignin biopolymers and release glucose and other fermentable sugars from recalcitrant plant cell wall carbohydrates, including cellulose and hemicellulose.  Here, we present the midgut transcriptome of larval Anoplophora glabripennis, a wood-boring beetle with documented lignin-, cellulose-, and hemicellulose- degrading capabilities, which provides valuable insights into how this insect overcomes challenges associated with feeding in woody tissue. Transcripts from putative protein coding regions of over 9,000 insect-derived genes were identified in the A. glabripennis midgut transcriptome using a combination of 454 shotgun and Illumina paired-end reads.  The most highly-expressed genes predicted to encode digestive-related enzymes were trypsins, carboxylesterases, β-glucosidases, and cytochrome P450s.  Furthermore, 180 unigenes predicted to encode glycoside hydrolases (GHs) were identified and included several GH 5, 45, and 48 cellulases, GH 1 xylanases, and GH 1 β-glucosidases.  In addition, transcripts predicted to encode enzymes involved in detoxification were detected, including a substantial number of unigenes classified as cytochrome P450s (CYP6B) and carboxylesterases, which are hypothesized to play pivotal roles in detoxifying host tree defensive chemicals and could make important contributions to A. glabripennis? expansive host range.  While a large diversity of insect-derived transcripts predicted to encode digestive and detoxification enzymes were detected, few transcripts predicted to encode enzymes required for lignin degradation or synthesis of essential nutrients were identified, suggesting that collaboration with microbial enzymes may be required for survival in woody tissue. A. glabripennis produces a number of enzymes with putative roles in cell wall digestion, detoxification, and nutrient extraction, which likely contribute to its ability to thrive in a broad range of host trees.  This system is quite different from the previously characterized termite fermentation system and provides new opportunities to discover enzymes that could be exploited for cellulosic ethanol biofuel production or the development of novel methods to control wood-boring pests. }
  }
  		

• Show RIS reference

  		TY  - JOUR
  ID  - 22500
  AU  - Scully,Erin D
  AU  - Hoover,Kelli 
  AU  - Carlson,John E
  AU  - Tien,Ming 
  AU  - Geib,Scott M
  T1  - Midgut Transcriptome Profiling of Anoplophora glabripennis, a Lignocellulose Degrading Cerambycid Beetle
  PY  - 2013
  KW  - cellulase
  KW  - carboxylesterase
  KW  - cytochrome P450
  KW  - xylanase
  KW  - comparative transcriptomics
  KW  - hemicellulose
  KW  - biofuels; beta-glucosidase
  UR  - http://www.biomedcentral.com/1471-2164/14/850
  N2  - Wood-feeding insects often work in collaboration with microbial symbionts to degrade lignin biopolymers and release glucose and other fermentable sugars from recalcitrant plant cell wall carbohydrates, including cellulose and hemicellulose.  Here, we present the midgut transcriptome of larval Anoplophora glabripennis, a wood-boring beetle with documented lignin-, cellulose-, and hemicellulose- degrading capabilities, which provides valuable insights into how this insect overcomes challenges associated with feeding in woody tissue. Transcripts from putative protein coding regions of over 9,000 insect-derived genes were identified in the A. glabripennis midgut transcriptome using a combination of 454 shotgun and Illumina paired-end reads.  The most highly-expressed genes predicted to encode digestive-related enzymes were trypsins, carboxylesterases, β-glucosidases, and cytochrome P450s.  Furthermore, 180 unigenes predicted to encode glycoside hydrolases (GHs) were identified and included several GH 5, 45, and 48 cellulases, GH 1 xylanases, and GH 1 β-glucosidases.  In addition, transcripts predicted to encode enzymes involved in detoxification were detected, including a substantial number of unigenes classified as cytochrome P450s (CYP6B) and carboxylesterases, which are hypothesized to play pivotal roles in detoxifying host tree defensive chemicals and could make important contributions to A. glabripennis? expansive host range.  While a large diversity of insect-derived transcripts predicted to encode digestive and detoxification enzymes were detected, few transcripts predicted to encode enzymes required for lignin degradation or synthesis of essential nutrients were identified, suggesting that collaboration with microbial enzymes may be required for survival in woody tissue. A. glabripennis produces a number of enzymes with putative roles in cell wall digestion, detoxification, and nutrient extraction, which likely contribute to its ability to thrive in a broad range of host trees.  This system is quite different from the previously characterized termite fermentation system and provides new opportunities to discover enzymes that could be exploited for cellulosic ethanol biofuel production or the development of novel methods to control wood-boring pests. 
  L3  - 10.1186/1471-2164-14-850
  JF  - BMC Genomics
  VL  - 14
  IS  - 850
  ER  -