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Citation for Study 16506

Citation title: "Production of potent antimicrobial agent by actinomycete, Streptomyces sannanensis strain SU118 isolated from phoomdi in Loktak lake of Manipur, India".

Study name: "Production of potent antimicrobial agent by actinomycete, Streptomyces sannanensis strain SU118 isolated from phoomdi in Loktak lake of Manipur, India".

This study is part of submission 16506 (Status: Published).

Citation

Singh L.S., Sharma H., & Talukdar N.C. 2014. Production of potent antimicrobial agent by actinomycete, Streptomyces sannanensis strain SU118 isolated from phoomdi in Loktak lake of Manipur, India. BMC Microbiology, .

Authors

• Singh L.S. (submitter) 09476299197
• Sharma H.
• Talukdar N.C.

Abstract

Background: Actinomycetes have provided a wealth of bioactive secondary metabolites with interesting activities such as antimicrobial, antiviral and anticancer. The study aims at isolation, characterization and the antimicrobial potentiality of Streptomyces sannanensis SU118 obtained from Phoomdi, a unique habitat of Loktak Lake of Manipur, India. Results: An actinomycete strain isolated from Phoomdi soil of Loktak Lake of Manipur, India was identified as Streptomyces sannanensis SU118. It is a Gram-positive filamentous bacterium which exhibits antimicrobial activity only against Gram-positive bacteria, while Gram-negative organisms were not affected. Glucose Soyabean meal broth was found to be the suitable medium for antibiotic production at 280 C for seven days of incubation. The antimicrobial agent produced by the strain was extracted with ethyl acetate as solvent and purified by thin layer chromatography. Screening and bioassay - guided fractionation of the ethyl acetate extract from the culture filtrate led to the isolation of an active potential compound (Rf value 0.56) with λmax 275.0 nm which has got the lowest minimum inhibitory concentration (0.5 ?g/ml) against Staphylococcus aureus MTCC 96 and Staphylococcus aureus (clinical isolate), whereas highest (3.0 ?g/ml) was recorded against Mycobacterium smegmatis MTCC 6 and Bacillus circulans MTCC 8074. Conclusion: This study has therefore uncovered the potential of exploring virgin untapped habitats in the Indo-Burma biodiversity hot spot region as reservoir of promising antimicrobial metabolite producer. These results highlighted the scope for further characterization of the metabolite and could be a candidate in the generation of new antimicrobial agents.

Keywords

Phoomdi, Streptomyces sannanensis SU118, Antibacterial potential, Minimum inhibitory concentration

External links

About this resource

• Canonical resource URI: http://purl.org/phylo/treebase/phylows/study/TB2:S16506
• Other versions: Nexus NeXML
• Show BibTeX reference

  		@ARTICLE{TreeBASE2Ref23760,
  	 author = {Laishram Shantikumar Singh and Hemant  Sharma and Narayan Chandra  Talukdar},
  	 title = {Production of potent antimicrobial agent by actinomycete, Streptomyces sannanensis strain SU118 isolated from phoomdi in Loktak lake of Manipur, India},
  	 year = {2014},
  	 keywords = {Phoomdi, Streptomyces sannanensis SU118, Antibacterial potential, Minimum inhibitory concentration},
  	 doi = {},
  	 url = {http://},
  	 pmid = {},
  	 journal = {BMC Microbiology},
  	 volume = {},
  	 number = {},
  	 pages = {},
  	 abstract = {Background: Actinomycetes have provided a wealth of bioactive secondary metabolites with interesting activities such as antimicrobial, antiviral and anticancer. The study aims at isolation, characterization and the antimicrobial potentiality of Streptomyces sannanensis SU118 obtained from Phoomdi, a unique habitat of Loktak Lake of Manipur, India.      
  Results: An actinomycete strain isolated from Phoomdi soil of Loktak Lake of Manipur, India was identified as Streptomyces sannanensis SU118. It is a Gram-positive filamentous bacterium which exhibits antimicrobial activity only against Gram-positive bacteria, while Gram-negative organisms were not affected. Glucose Soyabean meal broth was found to be the suitable medium for antibiotic production at 280 C for seven days of incubation. The antimicrobial agent produced by the strain was extracted with ethyl acetate as solvent and purified by thin layer chromatography. Screening and bioassay - guided fractionation of the ethyl acetate extract from the culture filtrate led to the isolation of an active potential compound (Rf value 0.56) with λmax 275.0 nm which has got the lowest minimum inhibitory concentration (0.5 ?g/ml) against Staphylococcus aureus MTCC 96 and Staphylococcus aureus (clinical isolate), whereas highest (3.0 ?g/ml) was recorded against Mycobacterium smegmatis MTCC 6 and Bacillus circulans MTCC 8074.
  Conclusion: This study has therefore uncovered the potential of exploring virgin untapped habitats in the Indo-Burma biodiversity hot spot region as reservoir of promising antimicrobial metabolite producer. These results highlighted the scope for further characterization of the metabolite and could be a candidate in the generation of new antimicrobial agents.
  }
  }
  		

• Show RIS reference

  		TY  - JOUR
  ID  - 23760
  AU  - Singh,Laishram Shantikumar
  AU  - Sharma,Hemant 
  AU  - Talukdar,Narayan Chandra 
  T1  - Production of potent antimicrobial agent by actinomycete, Streptomyces sannanensis strain SU118 isolated from phoomdi in Loktak lake of Manipur, India
  PY  - 2014
  KW  - Phoomdi
  KW  - Streptomyces sannanensis SU118
  KW  - Antibacterial potential
  KW  - Minimum inhibitory concentration
  UR  - http://dx.doi.org/
  N2  - Background: Actinomycetes have provided a wealth of bioactive secondary metabolites with interesting activities such as antimicrobial, antiviral and anticancer. The study aims at isolation, characterization and the antimicrobial potentiality of Streptomyces sannanensis SU118 obtained from Phoomdi, a unique habitat of Loktak Lake of Manipur, India.      
  Results: An actinomycete strain isolated from Phoomdi soil of Loktak Lake of Manipur, India was identified as Streptomyces sannanensis SU118. It is a Gram-positive filamentous bacterium which exhibits antimicrobial activity only against Gram-positive bacteria, while Gram-negative organisms were not affected. Glucose Soyabean meal broth was found to be the suitable medium for antibiotic production at 280 C for seven days of incubation. The antimicrobial agent produced by the strain was extracted with ethyl acetate as solvent and purified by thin layer chromatography. Screening and bioassay - guided fractionation of the ethyl acetate extract from the culture filtrate led to the isolation of an active potential compound (Rf value 0.56) with λmax 275.0 nm which has got the lowest minimum inhibitory concentration (0.5 ?g/ml) against Staphylococcus aureus MTCC 96 and Staphylococcus aureus (clinical isolate), whereas highest (3.0 ?g/ml) was recorded against Mycobacterium smegmatis MTCC 6 and Bacillus circulans MTCC 8074.
  Conclusion: This study has therefore uncovered the potential of exploring virgin untapped habitats in the Indo-Burma biodiversity hot spot region as reservoir of promising antimicrobial metabolite producer. These results highlighted the scope for further characterization of the metabolite and could be a candidate in the generation of new antimicrobial agents.
  
  L3  - 
  JF  - BMC Microbiology
  VL  - 
  IS  - 
  ER  -