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Citation for Study 17220

Citation title: "Enhanced Somatic embryogenesis in Theobroma cacao using the homologous BABYBOOM transcription factor".

Study name: "Enhanced Somatic embryogenesis in Theobroma cacao using the homologous BABYBOOM transcription factor".

This study is part of submission 17220 (Status: Published).

Citation

Florez S.L., Erwin R.L., Maximova S.N., Guiltinan M.J., & Curtis W.R. 2015. Enhanced Somatic embryogenesis in Theobroma cacao using the homologous BABYBOOM transcription factor. BMC Plant Biology, .

Authors

• Florez S.L.
• Erwin R.L.
• Maximova S.N.
• Guiltinan M.J.
• Curtis W.R.

Abstract

Introduction Theobroma cacao, the chocolate tree, is an important economic crop in East Africa, South East Asia, and South and Central America. Propagation of elite varieties has been achieved through somatic embryogenesis (SE) but low efficiencies and genotype dependence still represents a significant limitation for its propagation at commercial scales. Manipulation of transcription factors has been used to enhance the formation of SEs in several other plant species. This work describes the use of the transcription factor Babyboom (BBM) to promote the transition of somatic cacao cells from the vegetative to embryonic state. Results An ortholog of the Arabidopsis thaliana BBM gene (AtBBM) was characterized in T. cacao (TcBBM). TcBBM expression was observed throughout embryo development and was expressed at higher levels during SE as compared to zygotic embryogenesis (ZE). TcBBM overexpression in A. thaliana and T. cacao led to phenotypes associated with SE that did not require exogenous hormones. While transient ectopic expression of TcBBM provided only moderate enhancements in embryogenic potential, constitutive overexpression dramatically increased SE proliferation but also appeared to inhibit subsequent development. Conclusion Our work provides validation that TcBBM is an ortholog to AtBBM and has a specific role in both somatic and zygotic embryogenesis. Furthermore, our studies revealed that TcBBM transcript levels could serve as a biomarker for embryogenesis in cacao tissue. Results from transient expression of TcBBM provide confirmation that transcription factors can be used to enhance SE without compromising plant development. This strategy could compliment a hormone-based method of reprogramming somatic cells and lead to more precise manipulation of SE at the regulatory level of transcription factors. The technology would benefit the propagation of elite varieties with low regeneration potential as well as the production of transgenic plants, which similarly require somatic cell reprogramming.

Keywords

BABYBOOM, Somatic embryogenesis, Theobroma cacao, Cell reprogramming, plant propagation, transient gene expression

External links

About this resource

• Canonical resource URI: http://purl.org/phylo/treebase/phylows/study/TB2:S17220
• Other versions: Nexus NeXML
• Show BibTeX reference

  		@ARTICLE{TreeBASE2Ref24287,
  	 author = {Sergio L Florez and Rachel L Erwin and Siela N Maximova and Mark J Guiltinan and Wayne R Curtis},
  	 title = {Enhanced Somatic embryogenesis in Theobroma cacao using the homologous BABYBOOM transcription factor},
  	 year = {2015},
  	 keywords = {BABYBOOM, Somatic embryogenesis, Theobroma cacao, Cell reprogramming, plant propagation, transient gene expression},
  	 doi = {},
  	 url = {http://},
  	 pmid = {},
  	 journal = {BMC Plant Biology},
  	 volume = {},
  	 number = {},
  	 pages = {},
  	 abstract = {Introduction
  Theobroma cacao, the chocolate tree, is an important economic crop in East Africa, South East Asia, and South and Central America. Propagation of elite varieties has been achieved through somatic embryogenesis (SE) but low efficiencies and genotype dependence still represents a significant limitation for its propagation at commercial scales. Manipulation of transcription factors has been used to enhance the formation of SEs in several other plant species. This work describes the use of the transcription factor Babyboom (BBM) to promote the transition of somatic cacao cells from the vegetative to embryonic state. 
  Results
  An ortholog of the Arabidopsis thaliana BBM gene (AtBBM) was characterized in T. cacao (TcBBM).  TcBBM expression was observed throughout embryo development and was expressed at higher levels during SE as compared to zygotic embryogenesis (ZE). TcBBM overexpression in A. thaliana and T. cacao led to phenotypes associated with SE that did not require exogenous hormones. While transient ectopic expression of TcBBM provided only moderate enhancements in embryogenic potential, constitutive overexpression dramatically increased SE proliferation but also appeared to inhibit subsequent development. 
  Conclusion
  Our work provides validation that TcBBM is an ortholog to AtBBM and has a specific role in both somatic and zygotic embryogenesis. Furthermore, our studies revealed that TcBBM transcript levels could serve as a biomarker for embryogenesis in cacao tissue.  Results from transient expression of TcBBM provide confirmation that transcription factors can be used to enhance SE without compromising plant development.  This strategy could compliment a hormone-based method of reprogramming somatic cells and lead to more precise manipulation of SE at the regulatory level of transcription factors. The technology would benefit the propagation of elite varieties with low regeneration potential as well as the production of transgenic plants, which similarly require somatic cell reprogramming. 
  }
  }
  		

• Show RIS reference

  		TY  - JOUR
  ID  - 24287
  AU  - Florez,Sergio L
  AU  - Erwin,Rachel L
  AU  - Maximova,Siela N
  AU  - Guiltinan,Mark J
  AU  - Curtis,Wayne R
  T1  - Enhanced Somatic embryogenesis in Theobroma cacao using the homologous BABYBOOM transcription factor
  PY  - 2015
  KW  - BABYBOOM
  KW  - Somatic embryogenesis
  KW  - Theobroma cacao
  KW  - Cell reprogramming
  KW  - plant propagation
  KW  - transient gene expression
  UR  - http://dx.doi.org/
  N2  - Introduction
  Theobroma cacao, the chocolate tree, is an important economic crop in East Africa, South East Asia, and South and Central America. Propagation of elite varieties has been achieved through somatic embryogenesis (SE) but low efficiencies and genotype dependence still represents a significant limitation for its propagation at commercial scales. Manipulation of transcription factors has been used to enhance the formation of SEs in several other plant species. This work describes the use of the transcription factor Babyboom (BBM) to promote the transition of somatic cacao cells from the vegetative to embryonic state. 
  Results
  An ortholog of the Arabidopsis thaliana BBM gene (AtBBM) was characterized in T. cacao (TcBBM).  TcBBM expression was observed throughout embryo development and was expressed at higher levels during SE as compared to zygotic embryogenesis (ZE). TcBBM overexpression in A. thaliana and T. cacao led to phenotypes associated with SE that did not require exogenous hormones. While transient ectopic expression of TcBBM provided only moderate enhancements in embryogenic potential, constitutive overexpression dramatically increased SE proliferation but also appeared to inhibit subsequent development. 
  Conclusion
  Our work provides validation that TcBBM is an ortholog to AtBBM and has a specific role in both somatic and zygotic embryogenesis. Furthermore, our studies revealed that TcBBM transcript levels could serve as a biomarker for embryogenesis in cacao tissue.  Results from transient expression of TcBBM provide confirmation that transcription factors can be used to enhance SE without compromising plant development.  This strategy could compliment a hormone-based method of reprogramming somatic cells and lead to more precise manipulation of SE at the regulatory level of transcription factors. The technology would benefit the propagation of elite varieties with low regeneration potential as well as the production of transgenic plants, which similarly require somatic cell reprogramming. 
  
  L3  - 
  JF  - BMC Plant Biology
  VL  - 
  IS  - 
  ER  -