@ARTICLE{TreeBASE2Ref24934,
author = {Pradeep Kumar Divakar and Steve Leavitt and Carmen M. Molina and Ruth Del-Prado and H. Thorsten Lumbsch and Ana Crespo},
title = {A DNA barcoding approach for improving sample identification of hidden diversity in the lichen-forming fungal family Parmeliaceae (Ascomycota): Parmelia sensu stricto - a case study},
year = {2015},
keywords = {cryptic species ? DNA barcodes ? genetic distances ? ITS ? lichenized fungi.},
doi = {},
url = {http://},
pmid = {},
journal = {Botanical Journal of the Linnean Society},
volume = {180},
number = {1},
pages = {21?29},
abstract = {Accurate specimen identification is challenging groups with subtle or scarce taxonomically diagnostic characters, and DNA barcode identification can provide an effective means for consistent identification. Here, we investigate the utility of DNA barcode identification of species in a cosmopolitan genus of lichen-forming, Parmelia (Parmeliaceae). A total of 202 ITS sequences generated from specimens collected gathered from all continents, including Antarctica, were analyzed, and DNA barcodes of 14 species of Parmelia sensu stricto are reported. Almost all species show a barcode gap. Overall intra-specific divergence values were lower than the threshold established for Parmeliaceae previously. However, the mean and range was elevated by deep barcode divergences in three species, indicating occurrence of overlooked species. We here provide a DNA barcode reference library together with well-identified specimens sampled worldwide including Antarctica and sequences of most of the type material to both enable easy and fast accurate sample identification as well as to assist uncovering overlooked species in Parmelia s.s. Further, our results confirm the efficiency of internal transcribed spacer region (ITS) in the identification of Parmelia s.s. species. }
}
Citation for Study 18060
Citation title:
"A DNA barcoding approach for improving sample identification of hidden diversity in the lichen-forming fungal family Parmeliaceae (Ascomycota): Parmelia sensu stricto - a case study".
Study name:
"A DNA barcoding approach for improving sample identification of hidden diversity in the lichen-forming fungal family Parmeliaceae (Ascomycota): Parmelia sensu stricto - a case study".
This study is part of submission 18060
(Status: Published).
Citation
Divakar P.K., Leavitt S., Molina C., Del-prado R., Lumbsch H.T., & Crespo A. 2015. A DNA barcoding approach for improving sample identification of hidden diversity in the lichen-forming fungal family Parmeliaceae (Ascomycota): Parmelia sensu stricto - a case study. Botanical Journal of the Linnean Society, 180(1): 21?29.
Authors
-
Divakar P.K.
+34913942282
-
Leavitt S.
(submitter)
-
Molina C.
-
Del-prado R.
-
Lumbsch H.T.
-
Crespo A.
Abstract
Accurate specimen identification is challenging groups with subtle or scarce taxonomically diagnostic characters, and DNA barcode identification can provide an effective means for consistent identification. Here, we investigate the utility of DNA barcode identification of species in a cosmopolitan genus of lichen-forming, Parmelia (Parmeliaceae). A total of 202 ITS sequences generated from specimens collected gathered from all continents, including Antarctica, were analyzed, and DNA barcodes of 14 species of Parmelia sensu stricto are reported. Almost all species show a barcode gap. Overall intra-specific divergence values were lower than the threshold established for Parmeliaceae previously. However, the mean and range was elevated by deep barcode divergences in three species, indicating occurrence of overlooked species. We here provide a DNA barcode reference library together with well-identified specimens sampled worldwide including Antarctica and sequences of most of the type material to both enable easy and fast accurate sample identification as well as to assist uncovering overlooked species in Parmelia s.s. Further, our results confirm the efficiency of internal transcribed spacer region (ITS) in the identification of Parmelia s.s. species.
Keywords
cryptic species ? DNA barcodes ? genetic distances ? ITS ? lichenized fungi.
External links
About this resource
- Canonical resource URI:
http://purl.org/phylo/treebase/phylows/study/TB2:S18060
- Other versions:
Nexus
NeXML
- Show BibTeX reference
@ARTICLE{TreeBASE2Ref24934,
author = {Pradeep Kumar Divakar and Steve Leavitt and Carmen M. Molina and Ruth Del-Prado and H. Thorsten Lumbsch and Ana Crespo},
title = {A DNA barcoding approach for improving sample identification of hidden diversity in the lichen-forming fungal family Parmeliaceae (Ascomycota): Parmelia sensu stricto - a case study},
year = {2015},
keywords = {cryptic species ? DNA barcodes ? genetic distances ? ITS ? lichenized fungi.},
doi = {},
url = {http://},
pmid = {},
journal = {Botanical Journal of the Linnean Society},
volume = {180},
number = {1},
pages = {21?29},
abstract = {Accurate specimen identification is challenging groups with subtle or scarce taxonomically diagnostic characters, and DNA barcode identification can provide an effective means for consistent identification. Here, we investigate the utility of DNA barcode identification of species in a cosmopolitan genus of lichen-forming, Parmelia (Parmeliaceae). A total of 202 ITS sequences generated from specimens collected gathered from all continents, including Antarctica, were analyzed, and DNA barcodes of 14 species of Parmelia sensu stricto are reported. Almost all species show a barcode gap. Overall intra-specific divergence values were lower than the threshold established for Parmeliaceae previously. However, the mean and range was elevated by deep barcode divergences in three species, indicating occurrence of overlooked species. We here provide a DNA barcode reference library together with well-identified specimens sampled worldwide including Antarctica and sequences of most of the type material to both enable easy and fast accurate sample identification as well as to assist uncovering overlooked species in Parmelia s.s. Further, our results confirm the efficiency of internal transcribed spacer region (ITS) in the identification of Parmelia s.s. species. }
}
- Show RIS reference
TY - JOUR
ID - 24934
AU - Divakar,Pradeep Kumar
AU - Leavitt,Steve
AU - Molina,Carmen M.
AU - Del-Prado,Ruth
AU - Lumbsch,H. Thorsten
AU - Crespo,Ana
T1 - A DNA barcoding approach for improving sample identification of hidden diversity in the lichen-forming fungal family Parmeliaceae (Ascomycota): Parmelia sensu stricto - a case study
PY - 2015
KW - cryptic species ? DNA barcodes ? genetic distances ? ITS ? lichenized fungi.
UR - http://dx.doi.org/
N2 - Accurate specimen identification is challenging groups with subtle or scarce taxonomically diagnostic characters, and DNA barcode identification can provide an effective means for consistent identification. Here, we investigate the utility of DNA barcode identification of species in a cosmopolitan genus of lichen-forming, Parmelia (Parmeliaceae). A total of 202 ITS sequences generated from specimens collected gathered from all continents, including Antarctica, were analyzed, and DNA barcodes of 14 species of Parmelia sensu stricto are reported. Almost all species show a barcode gap. Overall intra-specific divergence values were lower than the threshold established for Parmeliaceae previously. However, the mean and range was elevated by deep barcode divergences in three species, indicating occurrence of overlooked species. We here provide a DNA barcode reference library together with well-identified specimens sampled worldwide including Antarctica and sequences of most of the type material to both enable easy and fast accurate sample identification as well as to assist uncovering overlooked species in Parmelia s.s. Further, our results confirm the efficiency of internal transcribed spacer region (ITS) in the identification of Parmelia s.s. species.
L3 -
JF - Botanical Journal of the Linnean Society
VL - 180
IS - 1
ER -
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