@ARTICLE{TreeBASE2Ref21694,
author = {Elizabeth Alvarez and Lederson Gañán and Alberto Rojas and Juan F Mejía and Germán A. Llano and Alonso Gonzalez},
title = {Diversity and pathogenicity of Colletotrichum species isolated from soursop in Colombia},
year = {2013},
keywords = {},
doi = {10.1007/s10658-014-0388-7},
url = {http://},
pmid = {},
journal = {European Journal of Plant Pathology},
volume = {},
number = {},
pages = {},
abstract = {Anthracnose, caused by Colletotrichum species is a highly limiting disease for the production of the tropical fruit tree crop, soursop (Annona muricata L.). In this study, 83 single-spore isolates of Colletotrichum were obtained from diseased soursoup tissues and subjected to a species complex-specific PCR assay. The isolates were identified as C. gloeosporioides sensu lato (n?=?60), C. boninense s. lat. (n?=?22), or C. acutatum s. lat. (n?=?1). A subset of 21 selected isolates was identified to species level by means of a multi-locus phylogenetic analysis using sequences from the ITS region and partial sequences of the actin, ?-tubulin-2, glyceraldehyde-3-phosphate dehydrogenase, and chitin synthase-1 genes. The multi-locus phylogenetic analysis resolved C. theobromicola, C. tropicale, C. siamense, and C. gloeosporioides sensu stricto in the C. gloeosporioides complex; C. karstii and one undetermined species in the C. boninense complex; as well as one undetermined species in the C. acutatum complex. Significant differences in anthracnose severity were observed between Colletotrichum species when tested for pathogenicity on attached twigs of soursop cv. Elita. Colletotrichum theobromicola and C. tropicale were associated with high and intermediate virulence, respectively, whereas the remaining species were associated with low virulence.}
}
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Citation title: "Diversity and pathogenicity of Colletotrichum species isolated from soursop in Colombia".
