TreeBASE Search-Analyses for study 18454

@ARTICLE{TreeBASE2Ref25250, author = {Washington A Salazar and Maria E Ordonez and Cristina Toapanta and Charles Wesley Barnes and Paul Gamboa}, title = {Ganoderma ecuadoriense W.A. Salazar, C.W. Barnes & Ord??ez, sp. nov.}, year = {2016}, keywords = {}, doi = {}, url = {http://}, pmid = {}, journal = {Persoonia}, volume = {36}, number = {}, pages = {}, abstract = {Ganoderma ecuadoriense Salazar, Barnes & Ord??ez, sp. nov. Etymology. Name reflects the geographical origin from which the fungus was collected. Basidiomata annual, flabelliform, pileate, pileus 21.65 x 21.88mm, surface glabrous, woody, reddish brown, laccate, upper surface covered by cinnamon colored powder of deposited basidiospores. Basidiospore surface smooth, white when fresh and dark brown when dry, pores, 5-7 per mm, round, thick walls. Stipe missing from sample, but lateral. Hyphal system dimitic, skeletal hyphae yellow to pale brown, 3.44-5.56 μm wide, end in ramifications, generative hyphae thin-walled, hyaline to pale yellow, 1.07-2.75 μm wide. Hyphae faintly amyloid when dispersed and slightly dextrinoid when in masses with Melzer. Cuticule cells club-like, slight amyloid reaction to 5% KOH and Melzer. Resin deposits between the trama and cuticle. Basidia not observed. Basidiospores hyaline to pale yellow, truncated, 8.09 -10.61 x 4.42 - 6.88 μm, no reaction to 5% KOH or Melzer. Typus. ECUADOR, Yasun? Research Station, Orellana Province, Ecuador, Mar. 2013, on decaying wood, Andr?s Salazar, holotype QCA(M)3430, ITS sequence GenBank XXX, Treebase XXX. Notes ? Morphological identification using the Neotropical Polyporaceae key (Ryvarden, 2004), showed G. ecuadoriense to be very close to G. perzonatum. However, Blastn ITS sequence comparisons, after trimming 18S and 28S sequence (Schoch et al., 2014), gave the top scores to Ganoderma orbiforme from Brazil, and G. cupreum, G. mastoporum, and G. fornicatum from China (Fig. 1). The sequence of Ganoderma sp. VPB202 from Brazil is actually identical, but has a low query score due to the discrepancy in sequence length, missing roughly 25 bases at the 3? end of ITS2. There were seven consistent differences, three in ITS1 and four in ITS2, between G. ecuadoriense, collected in the Northwestern Amazon basin in Ecuador, and the G. orbiforme, collected in the Southeastern Amazon in Brazil. Twenty sequences, 16 for G. ecuadoriense and four for G. orbiforme , were used in the DNA alignment analysis. The Ganoderma sp. VPB202 sequence suggests G. ecuadoriense occurs throughout the Amazon basin, but due to the missing bases of the ITS2 sequence, this is somewhat speculative.} }