TreeBASE Search-Matrices for study 10773

@ARTICLE{TreeBASE2Ref19135, author = {Laura Lowe Forrest and Noris Salazar Allen and Jose A Gudi?o and Helena Korpelainen and David G. Long}, title = {Molecular and morphological evidence for distinct species in Dumortiera (Dumortieraceae)}, year = {2011}, keywords = {Dumortiera, liverwort species, Central America, rbcL, psbA-trnH, rpoC1, ITS}, doi = {10.1639/0007-2745-114.1.102}, url = {http://www.bioone.org/doi/full/10.1639/0007-2745-114.1.102}, pmid = {}, journal = {The Bryologist}, volume = {114}, number = {1}, pages = {102--115}, abstract = {Contemporary taxonomists usually recognize a single species in Dumortiera, which is widely distributed through tropical and subtropical regions and extends into some temperate regions. Two subspecies, D. hirsuta subsp hirsuta and subsp. nepalensis, are also recognized in some treatments; both of these were thought to occur in Central America. We used DNA sequence data from the nuclear internal transcribed spacer region ITS, and three chloroplast loci (rbcL, psbA-trnH and rpoC1) to barcode Dumortiera across a wide geographic range, included a total of 50 accessions from the Americas, Asia and Europe. We also used light and scanning electron microscopy to examine morphological differences between accessions from Central America. Phylogenetic analyses of the sequence data show two highly distinct lineages. One appears to be restricted to Central America, while the other occurred in all the geographic regions sampled. This widespread lineage could itself be subdivided into several genetically and geographically distinct clades. The restricted Central American lineage contains plants that have a light green velvety appearance, due to an abundance of papillae on their upper thallus surface surrounded by elongated cells delimiting polygonal areas, and few to absence of marginal hairs. These can therefore be distinguished morphologically from Central American accessions from the widespread lineage, which are dark green, with a wide range of papillae densities (absent, few or abundant) on the thallus and usually with abundant marginal hairs. However, the wider application of these characters to lineages outside the Central American region has not yet been confirmed. We conclude that Dumortiera contains at least two, if not more, distinct species. However, we consider the application of the epithet nepalensis to plants in Central America premature until a reinvestigation of type material has been made. } }