TreeBASE Search-Matrices for study 11262

@ARTICLE{TreeBASE2Ref19513, author = {Eric M Adetutu and Krystal Thorpe and Steven Bourne and Xiangsheng Cao and Esmaeil Shahsavari and Greg Kirby and Andy S Ball}, title = {Phylogenetic Diversity of Fungal Communities in Tourist Accessible and Inaccessible Areas in Naracoorte Caves.}, year = {2011}, keywords = {DGGE, environmental factors, ITS regions, tourism }, doi = {}, url = {http://}, pmid = {}, journal = {Mycologia}, volume = {}, number = {}, pages = {}, abstract = {Abstract : The fungal diversity in tourist accessible and inaccessible areas at UNESCO World Heritage Listed Naracoorte Caves was investigated using culture dependent and independent techniques for assistance in cave management protocol development. The caves studied (Stick Tomato, Alexandra and Strawhaven Caves) were selected based on tourist numbers and configurations: Stick Tomato Cave (open, high tourist numbers), Alexandra Cave (lockable openings, high tourist numbers), and Strawhaven Cave (control; no tourist access). Culture based survey revealed Ascomycota dominance irrespective of sampling area with Microascales being most frequently isolated. Capnodiales, Hypocreales (Stick Tomato) and Eurotiales ?like (Alexandra) sequences were only cultured from tourist inaccessible areas. All these orders were also detected by DGGE assay irrespective of sampling area. The predominance of Ascomycota (especially Microascales) suggested their important ecological roles in these caves. Culture independent analysis showed higher Shannon fungal diversity values (from ITS based DGGE profiles) in tourist accessible areas of these caves than in inaccessible areas with the fungal community banding patterns being substantially different in Stick Tomato Cave. Further investigations are needed to determine the cause of the differences in the fungal communities of Stick Tomato Cave, although cave-related factors such as cave use, configuration and sediment heterogeneity might have contributed to these differences. } }