@ARTICLE{TreeBASE2Ref18202, author = {Alexandra H Wortley and P. J. Rudall and David J Harris and Robert W. Scotland}, title = {How Much Data are Needed to Resolve a Difficult Phylogeny? Case Study in Lamiales.}, year = {2005}, keywords = {}, doi = {10.1080/10635150500221028}, url = {}, pmid = {}, journal = {Systematic Biology}, volume = {54}, number = {5}, pages = {697--709}, abstract = {Reconstructing phylogeny is a crucial target of contemporary biology, now commonly approached through computerised analysis of genetic sequence data. In angiosperms, despite recent progress at the ordinal level, many relationships between families remain unclear. Here we take a case study from Lamiales, an angiosperm order in which interfamilial relationships have so far proved particularly problematic. We analyse the effect of changing one factor ? the quantity of sequence data analysed ? on phylogeny reconstruction in this group. We use simulation to estimate a priori the sequence data that would be needed to resolve an accurate, supported phylogeny of Lamiales. We investigate the effect of increasing the length of sequence data analysed, the rate of substitution in the sequences used, and of combining gene partitions. This method could be a valuable technique for planning systematic investigations in other problematic groups. Our results suggest that increasing sequence length is a better way to improve support, resolution and accuracy than employing sequences with a faster substitution rate. Indeed the latter may in some cases have detrimental effects on phylogeny reconstruction. Further molecular sequencing ? of at least 10,000 bp ? should result in a fully-resolved and supported phylogeny of Lamiales, but at present the problematic aspects of this tree model remain.} }

• Go to search
• Citation
• Taxa
• Matrices
• Trees
• Analyses
• 

CiteULike

Delicious

Connotea