TreeBASE Search-Matrices for study 14141

@ARTICLE{TreeBASE2Ref21929, author = {Dhanushka Udayanga and Lisa A. Castlebury and Amy Y. Rossman and Kevin D Hyde}, title = {Species limits in Diaporthe: molecular re-assessment of D. citri, D. cytosporella, D. foeniculina and D. rudis}, year = {2013}, keywords = {epitypification, genealogical sorting index, melanose, multi-gene phylogeny, primer design, Phomopsis, species recognition, stem end rot, systematics}, doi = {}, url = {http://}, pmid = {}, journal = {Persoonia}, volume = {}, number = {}, pages = {}, abstract = {Abstract Species of Diaporthe are important plant pathogens associated with a wide range of hosts worldwide. In the present study, the species associated with melanose and stem end rot diseases on Citrus spp. are revised. Morphology and molecular phylogenetic analyses of the complete nuclear ribosomal internal transcribed spacer regions and partial sequences of actin, beta-tubulin, calmodulin and translation elongation factor 1-alpha were used to resolve the species relationships and boundaries of species of Diaporthe. Three species of Diaporthe are associated with Citrus namely Diaporthe citri, Diaporthe cytosporella and Diaporthe foeniculina. Diaporthe citri occurs on Citrus throughout the Citrus-growing regions of the world. Diaporthe cytosporella is found on Citrus in Europe and California (USA). Diaporthe foeniculina, including the synonym D. neotheicola, is recognized as a species with an extensive host range including Citrus. Diaporthe medusaea, a name widely used for D. citri, was determined to be a synonym of D. rudis, a species with a broad host range. Diaporthe citri, D. cytosporella, D. foeniculina and D. rudis are epitypified, fully described and illustrated with a review of all synonyms based on molecular data and morphological studies. Newly designed primers are introduced to optimize the amplification and sequencing of calmodulin and actin genes in Diaporthe. A discussion is provided of the utility of ITS and EF1-α gene regions and the need for multi-gene phylogenies when distinguishing species of Diaporthe or describing new species. } }