TreeBASE Search-Matrices for study 14663

@ARTICLE{TreeBASE2Ref24314, author = {Xiang-Hua Wang and Bart Buyck and Annemieke Verbeken and Karen Hansen}, title = {Revisiting the morphology and phylogeny of Lactifluus with three new lineages from southern China}, year = {2015}, keywords = {Lactarius, lactiferous hyphae, Russulaceae, subtropical-tropical Asia, taxonomy}, doi = {}, url = {http://}, pmid = {}, journal = {Mycologia}, volume = {}, number = {}, pages = {}, abstract = {As a recent group mainly defined by molecular data, the genus Lactifluus is in high need of further study to provide insight into the morphological and molecular variation within the genus, species limits and relationships. Phylogenetic analyses of nuc rDNA ITS1-5.8S-ITS2 (ITS), D1 and D2 domains of nuc 28S rDNA (28S), and part of the second largest subunit of the RNA polymerase II (rpb2) (6?7 region) sequences of 28 samples from southern China revealed three new lineages of Lactifluus. Two of them are nested in a major clade that includes the type of Lactifluus, treated as two new sections: L. sect. Ambicystidiati and L. sect. Tenuicystidiati. Lactifluus ambicystidiati, described here as a new species (= sect. Ambicystidiati), has both lamprocystidia and macrocystidia in the hymenium, a unique combination of features within Russulaceae. Furthermore, only remnants of lactiferous hyphae are present in L. ambicystidiatus and our results suggest that the ability to form a lactiferous system has been lost in this lineage within Lactifluus. Lactifluus sect. Tenuicystidiati formed a strongly supported monophyletic group as a sister lineage to L. sect. Lactifluus (the L. volemus species complex). We recognize it based on the thin-walled macrocystidia and smaller ellipsoid spores. The former placement of L. tenuicystidiatus in the African L. sect. Pseudogymnocarpi was not supported. Using genealogical concordance we recognize five phylogenetic species within L. sect. Tenuicystidiati, and describe two of these as new, L. subpruinosus and L. tropicosinicus. The third lineage, represented by L. leoninus, formed a sister group to L. subg. Lactariopsis sensu stricto. The three lineages provide further evidence for morphological features in Lactifluus being homoplasious. Some sections and species complexes are likely to constitute more species and merit further investigations and subtropical-tropical Asia is likely a key region in accomplishing the sampling.} }