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@ARTICLE{TreeBASE2Ref16978, author = {Draginja Pavlic and Bernard Slippers and Teresa A. Coutinho and Michael J Wingfield}, title = {Identification of Botryosphaeria spp. from native Syzygium cordatum in South Africa}, year = {2006}, keywords = {}, doi = {}, url = {}, pmid = {}, journal = {Studies in Mycology}, volume = {}, number = {}, pages = {}, abstract = {Botryosphaeria spp. are widely distributed canker and die-back pathogens occurring on many forest tree species, including Eucalyptus (Myrtaceae). These fungi have recently been reported from native South African Myrtaceae, such as Syzygium cordatum and Heteropyxis natalensis. This finding initiated a survey of botryosphaeriaceous fungi from S. cordatum, which is the most common myrtaceous tree species in South Africa. Botryosphaeria strains were isolated from dying and asymptomatic twigs and leaves of S. cordatum from different geographical regions in South Africa. All isolates were induced to sporulate in culture and separated into eight groups based on conidial morphology. To facilitate their identification, representative isolates from these groups were compared with known Botryosphaeria spp. based on ITS rDNA sequence data. Phylogenetic analysis distinguished seven clades for the isolates obtained from S. cordatum. The ITS rDNA sequence data did not separate isolates of the cryptic species B. parva and B. ribis. These species could also not be distinguished confidently based on morphological data. A PCR-RFLP fingerprinting technique was, therefore, used to distinguish between isolates of these two species. From these data, eight Botryosphaeria spp. were identified including B. parva, B. ribis, B. lutea, B. australis, B. rhodina, B. dothidea, Fusicoccum mangiferum and Lasiodiplodia gonubiensis. Botryosphaeria ribis, B. parva and F. mangiferum were the most abundant species while only one isolate represented B. dothidea. The most abundant species on Syzygium are the same as those occurring on Eucalyptus and Mango trees in South Africa. It is clearly important for management programs to consider the potential impact of pathogens, such as Botryosphaeria, that can cross-infect native and introduced tree species.} }