@ARTICLE{TreeBASE2Ref26076, author = {Peter Montgomery Henry and Sharon Clark Kirkpatrick and Christina M Islas and Ana Maria Pastrana and Jenna Yoshisato and Steven T Koike and Oleg Daugovish and Thomas R Gordon}, title = {The Local Population and Methods for Detection of Fusarium oxysporum f. sp. fragariae, Cause of Fusarium Wilt of Strawberry, in California}, year = {2016}, keywords = {Fusarium oxysporum, strawberry, Fusarium, fragariae}, doi = {}, url = {http://}, pmid = {}, journal = {Plant Disease (APS)}, volume = {}, number = {}, pages = {}, abstract = {The objectives of this study were to investigate the structure of the population of Fusarium oxysporum f. sp. fragariae in California and to evaluate methods for its detection. Fifty-nine isolates of F. oxysporum f. sp. fragariae were obtained from diseased strawberry plants and their identity was confirmed by pathogenicity testing. The full intergenic spacer (IGS) and elongation factor 1-alpha (EF-1a) region were amplified by PCR and sequenced to elucidate phylogenetic relationships among isolates. IGS and EF-1a sequences revealed three main lineages, which corresponded to three somatic compatibility groups. Primers designed to detect F. oxysporum f. sp. fragariae in Japan amplified a 239 base pair product from 55 of 59 California isolates of F. oxysporum f. sp. fragariae and from no nonpathogenic isolates of F. oxysporum. The sequence of this PCR product was identical to the sequence obtained from F. oxysporum f. sp. fragariae isolates in Japan. Intensive sampling at two locations in California showed tests based on PCR and somatic compatibility to be in agreement (both positive or both negative) for 97% (257/264) of isolates tested. Our findings reveal considerable diversity in the California population of F. oxysporum f. sp. fragariae, which was most likely established through multiple introductions.} }

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