@ARTICLE{TreeBASE2Ref27204,
author = {Xuewen Xu and Minyang Chen and Jing Ji and Qiang Xu and Xiaohua Qi and Xuehao Chen},
title = {Comparative RNA-seq based transcriptome profiling of waterlogging response in cucumber hypocotyls reveals novel insights into the de novo adventitious root primordia initiation },
year = {2017},
keywords = {Cucumber, Waterlogging, Adventitious root primordia, RNA-seq, Ethylene},
doi = {},
url = {http://},
pmid = {},
journal = {BMC Plant Biology},
volume = {},
number = {},
pages = {},
abstract = {Background: Waterlogging is a serious impediment to plant growth because it results in the decline in the availability of oxygen to submerged tissues. Although cucumber (Cucumis sativus L.) is sensitive to waterlogging, its ability to produce adventitious roots (ARs) facilitates gas diffusion and increases plant survival when the oxygen concentration is decreased. To gain a better understanding of the molecular mechanisms that enable de novo AR primordia emergence upon waterlogging, the RNA sequencing-based transcriptomic responses of two contrasting cucumber genotypes, Zaoer-N (waterlogging tolerant) and Pepino (waterlogging sensitive), which differed in their abilities to form AR were compared.
Results: More than 27,000 transcripts were examined in cucumber hypocotyls, from which 1,494 and 1,766 genes in ?Zaoer-N? and ?Pepino?, respectively, were differentially expressed 2 days after waterlogging. The significant positive correlation between RNA sequencing data and a qPCR analysis indicated that the identified genes were credible. A comparative analysis revealed that genes functioning in carbohydrate mobilization, nitrate assimilation, hormone production and signaling pathways, transcription factors and cell division might contribute to the waterlogging-triggered AR primordia initiation. Ethylene was determined to be an important plant hormone responsible for the initiation of ARs in cucumber. Additionally, genes encoding cytochrome P450, ankyrin repeat-containing proteins and sulfite oxidases were determined as important in waterlogging acclimation.
Conclusion: This research broadens our understanding of the mechanism underlying waterlogging-triggered ARs emergence, and provides valuable information for the breeding of cucumber with enhanced waterlogging tolerance.}
}
Matrices for Study 21003
Citation title:
"Comparative RNA-seq based transcriptome profiling of waterlogging response in cucumber hypocotyls reveals novel insights into the de novo adventitious root primordia initiation ".
Study name:
"Comparative RNA-seq based transcriptome profiling of waterlogging response in cucumber hypocotyls reveals novel insights into the de novo adventitious root primordia initiation ".
This study is part of submission 21003
(Status: Published).
Matrices
Go to search
Citation
Taxa
Matrices
Trees
Analyses
