@ARTICLE{TreeBASE2Ref28700, author = {Marco Antonio Bautista-Cruz and Gustavo Almaguer-Vargas and Santos Gerardo Leyva-Mir and Maria Teresa Colinas-Le?n and Kamila Camara Correia and Mois?s Camacho-Tapia and Leticia Robles-Yerena and Sami Jorge Michereff and Juan Manoel Tovar-Pedraza}, title = {Phylogeny, Distribution, and Pathogenicity of Lasiodiplodia Species Associated With Cankers and Dieback Symptoms of Persian Lime in Mexico.}, year = {2019}, keywords = {Citrus latifolia, dieback, pathogenicity, virulence, phylogenetic inference}, doi = {10.1094/PDIS-06-18-1036-RE}, url = {http://apsjournals.apsnet.org/doi/10.1094/PDIS-06-18-1036-RE}, pmid = {}, journal = {Plant Disease}, volume = {}, number = {}, pages = {}, abstract = {Persian lime (Citrus latifolia Tan.) is an important and widely cultivated fruit crop in several regions of Mexico. In recent years, severe symptoms of gummosis, stem cankers, and dieback were detected in the Persian lime-producing region in the states of Veracruz and Puebla, Mexico. The aims of this study were to identify the species of Lasiodiplodia associated with these symptoms, determine the distribution of these species and test their pathogenicity and virulence on Persian lime plants. In 2015, symptomatic samples of 12 commercial Persian lime orchards were collected and 60 Lasiodiplodia isolates were obtained. Fungal identification of 32 representative isolates was performed using a phylogenetic analysis based on DNA sequence data of the internal transcribed spacer (ITS) region, and part of the translation elongation factor 1-α (EF1-α), and β-tubulin (BT2) genes. Sequence analysis was carried out using the Maximum Likelihood and Bayesian Inference methods. Six Lasiodiplodia species were identified as L. pseudotheobromae, L. theobromae, L. brasiliense, L. subglobosa, L. citricola and L. iraniensis. All Lasiodiplodia species of this study are reported for first time in association with Persian lime in Mexico and worldwide. Lasiodiplodia pseudotheobromae (42% of isolates) was the most frequently isolated species, followed by L. theobromae (28%) and L. brasiliense (12.5%). Pathogenicity on Persian lime young plant using a mycelial plug inoculation method showed that all identified Lasiodiplodia species were able to cause necrotic lesions and gummosis, but L. subglobosa, L. iraniensis, and L. pseudotheobromae were the most virulent.} }

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