TreeBASE Search-Matrices for study 24165

@ARTICLE{TreeBASE2Ref29415, author = {Minna Annina Launis and Jiř? Mal?ček and M?ns Svensson and Andrei Tsurykau and Emmanu?l S?rusiaux and Leena Myllys}, title = {Sharpening species boundaries in the Micarea prasina group with notes on the type species M. prasina}, year = {2019}, keywords = {crystalline granules; diversity; dead wood; epitypes; lichens; molecular phylogenetics; new species; species delimitation; taxonomy; type species. }, doi = {}, url = {http://}, pmid = {}, journal = {Mycologia}, volume = {}, number = {}, pages = {}, abstract = {Micarea is a lichenized genus in the family Pilocarpaceae (Ascomycota). We studied the phylogeny and reassessed the current taxonomy of the M. prasina group. We focused especially on the taxonomic questions concerning the type species M. prasina and, furthermore, challenges concerning type specimens that are too old for successful DNA barcoding and molecular studies. The phylogeny was reconstructed using nuc rDNA internal transcribed spacer region (ITS1-5.8S-ITS2), mito rDNA small subunit (mtSSU) and replication licensing factor MCM7 gene from 31 taxa species. Fifty-six new sequences were generated. The data was analyzed using maximum parsimony and maximum likelihood methods. The results revealed four undescribed, well-supported lineages. Three lineages represent new species described here as M. fallax, M. flavoleprosa and M. pusilla. In addition, our results support the recognition of M. melanobola as a distinct species. Micarea fallax is characterized by a vivid to olive green thallus composed of aggregated granules and whitish or brownish apothecia sometimes with greyish tinge (Sedifolia-grey pigment). Micarea flavoleprosa has a thick, wide-spreading yellowish-, whitish to olive-green sorediate thallus and lacks the Sedifolia-grey pigmentation. The species is mostly anamorphic, developing apothecia rarely. Micarea melanobola is characterized by a pale to dark vivid green granular thallus and darkly pigmented apothecia (Sedifolia-grey). Micarea pusilla is characterized by a whitish-green to olive-green thinly granular or membranous thallus, numerous and very small whitish apothecia lacking the Sedifolia-grey pigment, and by the production of methoxymicareic acid. Micarea fallax, M. flavoleprosa and M. melanobola produce micareic acid. The reliability of crystalline granules as a character for species delimitation was investigated and was highly informative for linking the old type specimen of M. prasina to fresh material.} }