@ARTICLE{TreeBASE2Ref30477, author = {Xiaodong Zheng and Yuxiong Xiao and Yike Tian and Shaolan Yang and Caihong Wang}, title = {PcDWF1, a Pear Brassinosteroid Biosynthetic Gene Homologous to AtDWARF1, Affected the Vegetative and Reproductive Growth of Plants}, year = {2020}, keywords = {Brassinosteroids, PcDWF1, Vegetative and Reproductive Growth, Pyrus ussuriensis, Nicotiana tabacum}, doi = {}, url = {http://}, pmid = {}, journal = {BMC Plant Biology}, volume = {}, number = {}, pages = {}, abstract = {Background: The steroidal hormones brassinosteroids (BRs) play important roles in plant growth and development. The pathway and genes involved in BR biosynthesis have been identified primarily in model plants like Arabidopsis, but little is known about BR biosynthesis in woody fruits such as pear. Results: In this study, we found that applying exogenous brassinolide (BL) could significantly increase the stem growth and rooting ability of Pyrus ussuriensis. PcDWF1, which had a significantly lower level of expression in the dwarf-type pear than in the standard-type pear, was cloned for further analysis. A phylogenetic analysis showed that PcDWF1 was a pear brassinosteroid biosynthetic gene that was homologous to AtDWARF1. The subcellular localization analysis indicated that PcDWF1 was located in the plasma membrane. Overexpression of PcDWF1 in tobacco (Nicotiana tabacum) or pear (Pyrus ussuriensis) plants promoted the growth of the stems, which was caused by a larger cell size and more developed xylem than those in the control plants, and the rooting ability was significantly enhanced. In addition to the change in vegetative growth, the tobacco plants overexpressing PcDWF1 also had a delayed flowering time and larger seed size than did the control tobacco plants. These phenotypes were considered to result from the higher BL contents in the transgenic lines than in the control tobacco and pear plants. Conclusions: Taken together, these results reveal that the pear BR biosynthetic gene PcDWF1 affected the vegetative and reproductive growth of Pyrus ussuriensis and Nicotiana tabacum and could be characterized as an important BR biosynthetic gene in perennial woody fruit plants.} }

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