@ARTICLE{TreeBASE2Ref30884, author = {D Rabern Simmons and Anne E Bonds and Buck T Castillo and Rebecca A Clemons and Alex D Glasco and Jillian M Myers and Natasha Thapa and Peter M Letcher and Martha J Powell and Joyce E Longcore and Timothy Y. James}, title = {The Collection of Zoosporic Eufungi at the University of Michigan (CZEUM): Introducing a new repository of fully barcoded Chytridiomyceta and Blastocladiomycota cultures}, year = {2020}, keywords = {cryopreservation; Oxford Nanopore; phylogeny}, doi = {10.1186/s43008-020-00041-z}, url = {http://}, pmid = {}, journal = {IMA Fungus}, volume = {11}, number = {}, pages = {20}, abstract = {We formed the Collection of Zoosporic Eufungi at the University of Michigan (CZEUM) in 2018 as a cryopreserved fungal collection consolidating the University of Maine Culture Collection (UMCC, or JEL), the University of Alabama Chytrid Culture Collection (UACCC), and additional zoosporic eufungal accessions. The CZEUM is publicly accessible and contains 1044 cryopreserved cultures of Chytridiomycota, Monoblepharidomycota, and Blastocladiomycota, with 51 cultures being ex-type strains. We molecularly characterized 431 cultures by amplifying the majority of the rDNA operon in a single reaction, yielding an average fragment length of 4739 bp. We sequenced multiplexed samples with an Oxford Nanopore Technology MinION device and software, which was capable of producing sequences identical to published Sanger sequences. With this data, we generated a phylogeny with an additional 451 published zoosporic eufungi sequences to produce the most comprehensive phylogeny of these taxa to date. The CZEUM is thus largely characterized by molecular data, which can guide instructors and researchers on future studies of these organisms. Cultures from the CZEUM can be purchased by contacting the collection curator.} }

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