@ARTICLE{TreeBASE2Ref15474, author = {Timothy P. Friedlander and Jerome C. Regier and Charles Mitter and David L. Wagner}, title = {A nuclear gene for higher level phylogenetics: Phosphoenolpyruvate carboxykinase tracks Mesozoic-age divergences within Lepidoptera (Insecta).}, year = {1996}, keywords = {}, doi = {}, url = {http://mbe.oxfordjournals.org/cgi/content/abstract/13/4/594}, pmid = {}, journal = {Molecular Biology and Evolution}, volume = {13}, number = {4}, pages = {594--604}, abstract = {The sequence of phosphoenolpyruvate carboxykinase (PEPCK) has been previously identified as a promising candidate for reconstructing Mesozoic-age divergences (Friedlander, Regier, and Mitter 1992, 1994). To test this hypothesis more rigorously, 597 nucleotides of aligned PEPCK coding sequence (similar to 30% of the coding region) were generated from 18 species representing Mesozoic-age lineages of moths (Insects: Lepidoptera) and outgroup taxa. Relationships among basal Lepidoptera are well established by morphological analysis, providing a strong test for the utility of a gene which has not previously been used in systematics. Parsimony and other phylogenetic analyses were conducted on nucleotides by codon positions (nt1, nt2, nt3) separately and in combination, and on amino acids, for comparison to the test phylogeny. The highest concordance was achieved with nt1 + nt2, for which one of two most-parsimonious frees was identical to the test phylogeny, and with all nucleotides when nt3 was downweighted sevenfold or higher, for which a single most-parsimonious tree identical to the test phylogeny resulted. Substitutions in nt3 approached saturation in many, but not all, pairwise comparisons and their exclusion or severe downweighting greatly increased the degree of concordance with the test phylogeny. Neighbor-joining analysis confirms this finding. The utility of PEPCK for phylogenetics is demonstrated over a time span for which few other suitable genes are currently available.} }

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