@ARTICLE{TreeBASE2Ref23490, author = {Tiago de Souza Leite and Carla Aparecida de Oliveira Castro and Olinto Liparini Pereira and Elza Fernandes de Araujo and Marisa Vieira de Queiroz}, title = {Enrichment and characterization of basidiomycetous endophytes associated with soybeans and coffee tissues}, year = {2014}, keywords = {benomyl; endophytes; tolerant; filamentous fungi; yeasts}, doi = {}, url = {http://}, pmid = {}, journal = {}, volume = {}, number = {}, pages = {}, abstract = {Most fungal endophytes associated with plant tissues are representatives of the phylum Ascomycota. However, representatives of the phylum Basidiomycota can also be found in plant tissues, at a higher frequency than traditional isolation methods have demonstrated thus far. In this study, we used the fungicide benomyl, as a semi-selective agent, to improve the isolation of basidiomycetous filamentous fungi and yeasts from soybean and coffee tissues. From a total of 851 CFU.g-1 of tissue, 58 taxa were identified, of which 3 were basidiomycetous filamentous fungi (Coprinellus radians, Marasmius tenuissimus and Peniophora sp.); 19 were basidiomycetous yeasts (genera: Bulleromyces, Cryptococcus, Erythrobasidium, Fellomyces, Hannaella, Kurtzmanomyces, Occultifur, Rhodotorula and Sporidiobolus); 18 were fungal species of the Order Pleosporales (Phylum Ascomycota), previously reported to be tolerant to benomyl, 3 were ascomycetous yeasts and another 15 were also representatives of the phylum Ascomycota. A total of 43, i.e., 74%, of the taxa were representatives of basidiomycetous fungi and ascomycetous fungi, known to be more resistant to benomyl. Additionally, 22 of the 58 taxa, i.e., 38%, were basidiomycetous fungi (3 taxa) and yeasts (19 taxa), a significant proportion compared to data reported in the literature. There was no significant difference in fungi or yeasts diversity among soybean tissues, but the coffee branches exhibited greater diversity compared to coffee leaves. The possible ecological role of these basidiomycetous fungi and yeasts, their potential and prospects for their study are discussed. To our knowledge, this is the first study that uses benomyl, to improve the isolation of basidiomycetous endophytes} }

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