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Citation for Study 10089

About Citation title: "Molecular evolution and phylogenetics of complete chloroplast genomes in Poaceae.".
About This study was previously identified under the legacy study ID S2429 (Status: Published).

Citation

Duvall M.R., Leseberg C., Grennan C., & Morris L. 2008. "Molecular evolution and phylogenetics of complete chloroplast genomes in Poaceae." In: Seberg O., Petersen G., Barfod A.S., & Davis J.I., eds. Fifth International Symposium on Grass Systematics and Evolution. pp. . Copenhagen, Aarhus University Press.

Authors

  • Duvall M.R.
  • Leseberg C.
  • Grennan C.
  • Morris L.

Abstract

Phylogenetic issues in Poaceae not resolved by previous multi-gene analyses can be usefully investigated by small genome-scale analyses. In this pilot study, complete or nearly complete chloroplast genomes (plastomes) were sequenced from six selected graminoids. Representatives of Anomochlooideae, Puelioideae, Bambusoideae, both major tribes of Panicoideae and Joinvilleaceae were newly sampled to supplement previously published plastome data from Ehrhartoideae, Pooideae, and Andropogoneae. For amplification and sequencing, over 200 pairs of primers were designed in conserved regions of published grass plastomes that were positioned to flank overlapping 1200-base pair fragments around the entire plastome. As expected, gene order and number were highly conserved. Concurrent with the high conservation of the plastome was considerable cumulative variation useful for studies within the family and even within a single tribe. Readily interpreted mutational patterns were observed, such as small inversions of the loop in hairpin-loop regions and indels resulting from slipped-strand mispairings. Phylogenetic analyses were conducted on these and eight previously published plastomes. Maximum or near-maximum support was observed in all likelihood and parsimony bootstrap analyses including shallow nodes, such as those within a clade corresponding to a complex of four Andropogoneae, and deep nodes, such as the one uniting the bambusoid/ehrhartoid/pooid (BEP) clade.

About this resource

  • Canonical resource URI: http://purl.org/phylo/treebase/phylows/study/TB2:S10089
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