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Citation for Study 10246

About Citation title: "Concatenated SSU and LSU rDNA data confirm the main evolutionary trends within myxosporeans (Myxozoa: Myxosporea) and provide an effective tool for their molecular phylogenetics.".
About This study was previously identified under the legacy study ID S2603 (Status: Published).

Citation

Bartosova P., Fiala I., & Hypsa V. 2009. Concatenated SSU and LSU rDNA data confirm the main evolutionary trends within myxosporeans (Myxozoa: Myxosporea) and provide an effective tool for their molecular phylogenetics. Molecular Phylogenetics and Evolution, 53(1): 81-93.

Authors

  • Bartosova P.
  • Fiala I.
  • Hypsa V.

Abstract

Views on myxosporean phylogeny and systematics have recently undergone substantial changes resulting from analyses of SSU rDNA. Here, we further investigate the evolutionary trends within myxosporean lineages by using 35 new sequences of the LSU rDNA. We show a good agreement between the two rRNA genes and confirm the main phylogenetic split between the freshwater and marine lineages. The informative superiority of the LSU data is shown by an increase of the resolution, nodal supports and tree indexes in the LSU rDNA and combined analyses. We determine the most suitable part of LSU for the myxosporean phylogeny by comparing informative content in various regions of the LSU sequences. Based on this comparison, we propose the D530-end part of the LSU rRNA gene as the most informative region which provides in concatenation with the complete SSU a well resolved and robust tree. To allow for simple amplification of the marker, we design specific primer set for this part of LSU rDNA.

Keywords

Myxosporea; Phylogeny; LBA; LSU rDNA; 28S; SSU rDNA; 18S; D domains

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About this resource

  • Canonical resource URI: http://purl.org/phylo/treebase/phylows/study/TB2:S10246
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