CiteULike

Delicious

Connotea

Citation for Study 1049

Citation title: "Identifying pine-inhabiting Lophodermium species using PCR-RFLP.".

This study was previously identified under the legacy study ID S944 (Status: Published).

Citation

Johnston P., Park D., Dick M., Ortiz-garc?a S., & Gernandt D. 2003. Identifying pine-inhabiting Lophodermium species using PCR-RFLP. New Zealand Journal of Forestry Science, 33: 10-24.

Authors

• Johnston P. +64 9 574 4714
• Park D.
• Dick M.
• Ortiz-garc?a S.
• Gernandt D.

Abstract

Three species of Lophodermium are previously reported from pine in New Zealand?L. conigenum, L. pinastri, and the newly reported L. molitoris. All are saprobes with an initial endophytic phase. The North American species L. seditiosum is considered a potential threat to New Zealand's plantation forests. Because these fungi are difficult to distinguish morphologically, a simple molecular method was developed to distinguish the four species. The ITS region is digested using HaeIII and HpaII in a single reaction, resulting in unique RFLP banding patterns for each of the species treated. Two genetically distinct groups were recognised within Lophodermium pinastri, one North American and one European. All the New Zealand isolates of L. pinastri tested were identical to the North American group.

About this resource

• Canonical resource URI: http://purl.org/phylo/treebase/phylows/study/TB2:S1049
• Other versions: Nexus NeXML
• Show BibTeX reference

  		@ARTICLE{TreeBASE2Ref16022,
  	 author = {Peter  Johnston and Duckchul  Park and Margaret A  Dick and Sol  Ortiz-Garc?a and David S.  Gernandt},
  	 title = {Identifying pine-inhabiting Lophodermium species using PCR-RFLP.},
  	 year = {2003},
  	 keywords = {},
  	 doi = {},
  	 url = {},
  	 pmid = {},
  	 journal = {New Zealand Journal of Forestry Science},
  	 volume = {33},
  	 number = {},
  	 pages = {10--24},
  	 abstract = {Three species of Lophodermium are previously reported from pine in New Zealand?L. conigenum, L. pinastri, and the newly reported L. molitoris. All are saprobes with an initial endophytic phase. The North American species L. seditiosum is considered a potential threat to New Zealand's plantation forests. Because these fungi are difficult to distinguish morphologically, a simple molecular method was developed to distinguish the four species. The ITS region is digested using HaeIII and HpaII in a single reaction, resulting in unique RFLP banding patterns for each of the species treated. Two genetically distinct groups were recognised within Lophodermium pinastri, one North American and one European. All the New Zealand isolates of L. pinastri tested were identical to the North American group.}
  }
  		

• Show RIS reference

  		TY  - JOUR
  ID  - 16022
  AU  - Johnston,Peter 
  AU  - Park,Duckchul 
  AU  - Dick,Margaret A 
  AU  - Ortiz-Garc?a,Sol 
  AU  - Gernandt,David S. 
  T1  - Identifying pine-inhabiting Lophodermium species using PCR-RFLP.
  PY  - 2003
  UR  - 
  N2  - Three species of Lophodermium are previously reported from pine in New Zealand?L. conigenum, L. pinastri, and the newly reported L. molitoris. All are saprobes with an initial endophytic phase. The North American species L. seditiosum is considered a potential threat to New Zealand's plantation forests. Because these fungi are difficult to distinguish morphologically, a simple molecular method was developed to distinguish the four species. The ITS region is digested using HaeIII and HpaII in a single reaction, resulting in unique RFLP banding patterns for each of the species treated. Two genetically distinct groups were recognised within Lophodermium pinastri, one North American and one European. All the New Zealand isolates of L. pinastri tested were identical to the North American group.
  L3  - 
  JF  - New Zealand Journal of Forestry Science
  VL  - 33
  IS  - 
  SP  - 10
  EP  - 24
  ER  -