@ARTICLE{TreeBASE2Ref19441,
author = {Angelique Helena Hoolahan and Vivian C Blok and Tracey Gibson and Mark Dowton},
title = {A Comparison of Three Molecular Markers for the Identification of Populations of Globodera pallida},
year = {2011},
keywords = {Diagnostics, genetics, genetic distance, Globodera pallida, internal transcribed spacer, mitochondrial DNA, phylogenetics, potato cyst nematode, rbp-1, systematics},
doi = {},
url = {http://},
pmid = {},
journal = {Journal of Nematology},
volume = {},
number = {},
pages = {},
abstract = {Potato cyst nematodes cost the potato industry substantial financial losses annually. Through the use of molecular markers, the distribution and infestation routes of these nematodes can be better elucidated, permitting the development of more effective preventative methods. Here we assess the ability of three molecular markers to resolve multiple representatives of five Globodera pallida populations as monophyletic groups. Molecular markers included a region of the rbp-1 gene (a pathogenicity factor), a non-coding nuclear DNA region (the ITS region), and a novel marker for G. pallida, a ~3.4 kb non-coding mitochondrial DNA (mtDNA) region. Multiple phylogenetic analysis methods were performed on the three DNA regions separately, and on a data set of these three regions combined. The analyses of the combined data set were similar to that of the sole mtDNA marker; resolving more populations as monophyletic groups, relative to that of the ITS region and rbp-1 gene region. This suggests that individual markers may be inadequate for distinguishing populations of G. pallida. The use of this new non-coding mtDNA marker may provide further insights into the historical distribution of G. pallida, as well as enable the development of more sensitive diagnostic methods.}
}
Citation for Study 11163
Citation title:
"A Comparison of Three Molecular Markers for the Identification of Populations of Globodera pallida".
Study name:
"A Comparison of Three Molecular Markers for the Identification of Populations of Globodera pallida".
This study is part of submission 11153
(Status: Published).
Citation
Hoolahan A.H., Blok V.C., Gibson T., & Dowton M. 2011. A Comparison of Three Molecular Markers for the Identification of Populations of Globodera pallida. Journal of Nematology, .
Authors
-
Hoolahan A.H.
(submitter)
+61 2 4298 1992
-
Blok V.C.
-
Gibson T.
-
Dowton M.
Abstract
Potato cyst nematodes cost the potato industry substantial financial losses annually. Through the use of molecular markers, the distribution and infestation routes of these nematodes can be better elucidated, permitting the development of more effective preventative methods. Here we assess the ability of three molecular markers to resolve multiple representatives of five Globodera pallida populations as monophyletic groups. Molecular markers included a region of the rbp-1 gene (a pathogenicity factor), a non-coding nuclear DNA region (the ITS region), and a novel marker for G. pallida, a ~3.4 kb non-coding mitochondrial DNA (mtDNA) region. Multiple phylogenetic analysis methods were performed on the three DNA regions separately, and on a data set of these three regions combined. The analyses of the combined data set were similar to that of the sole mtDNA marker; resolving more populations as monophyletic groups, relative to that of the ITS region and rbp-1 gene region. This suggests that individual markers may be inadequate for distinguishing populations of G. pallida. The use of this new non-coding mtDNA marker may provide further insights into the historical distribution of G. pallida, as well as enable the development of more sensitive diagnostic methods.
Keywords
Diagnostics, genetics, genetic distance, Globodera pallida, internal transcribed spacer, mitochondrial DNA, phylogenetics, potato cyst nematode, rbp-1, systematics
External links
About this resource
- Canonical resource URI:
http://purl.org/phylo/treebase/phylows/study/TB2:S11163
- Other versions:
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NeXML
- Show BibTeX reference
@ARTICLE{TreeBASE2Ref19441,
author = {Angelique Helena Hoolahan and Vivian C Blok and Tracey Gibson and Mark Dowton},
title = {A Comparison of Three Molecular Markers for the Identification of Populations of Globodera pallida},
year = {2011},
keywords = {Diagnostics, genetics, genetic distance, Globodera pallida, internal transcribed spacer, mitochondrial DNA, phylogenetics, potato cyst nematode, rbp-1, systematics},
doi = {},
url = {http://},
pmid = {},
journal = {Journal of Nematology},
volume = {},
number = {},
pages = {},
abstract = {Potato cyst nematodes cost the potato industry substantial financial losses annually. Through the use of molecular markers, the distribution and infestation routes of these nematodes can be better elucidated, permitting the development of more effective preventative methods. Here we assess the ability of three molecular markers to resolve multiple representatives of five Globodera pallida populations as monophyletic groups. Molecular markers included a region of the rbp-1 gene (a pathogenicity factor), a non-coding nuclear DNA region (the ITS region), and a novel marker for G. pallida, a ~3.4 kb non-coding mitochondrial DNA (mtDNA) region. Multiple phylogenetic analysis methods were performed on the three DNA regions separately, and on a data set of these three regions combined. The analyses of the combined data set were similar to that of the sole mtDNA marker; resolving more populations as monophyletic groups, relative to that of the ITS region and rbp-1 gene region. This suggests that individual markers may be inadequate for distinguishing populations of G. pallida. The use of this new non-coding mtDNA marker may provide further insights into the historical distribution of G. pallida, as well as enable the development of more sensitive diagnostic methods.}
}
- Show RIS reference
TY - JOUR
ID - 19441
AU - Hoolahan,Angelique Helena
AU - Blok,Vivian C
AU - Gibson,Tracey
AU - Dowton,Mark
T1 - A Comparison of Three Molecular Markers for the Identification of Populations of Globodera pallida
PY - 2011
KW - Diagnostics
KW - genetics
KW - genetic distance
KW - Globodera pallida
KW - internal transcribed spacer
KW - mitochondrial DNA
KW - phylogenetics
KW - potato cyst nematode
KW - rbp-1
KW - systematics
UR - http://dx.doi.org/
N2 - Potato cyst nematodes cost the potato industry substantial financial losses annually. Through the use of molecular markers, the distribution and infestation routes of these nematodes can be better elucidated, permitting the development of more effective preventative methods. Here we assess the ability of three molecular markers to resolve multiple representatives of five Globodera pallida populations as monophyletic groups. Molecular markers included a region of the rbp-1 gene (a pathogenicity factor), a non-coding nuclear DNA region (the ITS region), and a novel marker for G. pallida, a ~3.4 kb non-coding mitochondrial DNA (mtDNA) region. Multiple phylogenetic analysis methods were performed on the three DNA regions separately, and on a data set of these three regions combined. The analyses of the combined data set were similar to that of the sole mtDNA marker; resolving more populations as monophyletic groups, relative to that of the ITS region and rbp-1 gene region. This suggests that individual markers may be inadequate for distinguishing populations of G. pallida. The use of this new non-coding mtDNA marker may provide further insights into the historical distribution of G. pallida, as well as enable the development of more sensitive diagnostic methods.
L3 -
JF - Journal of Nematology
VL -
IS -
ER -
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