@ARTICLE{TreeBASE2Ref19603,
author = {Angelique Corthals and Antonius Koller and Dwight Martin and Robert Rieger and Emily Chen and Mario Bernaski and Gabriella Recagno and Liliana M Davalos},
title = {Detecting the Immune System Response of a 500 Year-old Inca Mummy},
year = {2012},
keywords = {forensic DNA, immune system, proteomics, SH test.},
doi = {10.1371/journal.pone.0041244},
url = {http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0041244},
pmid = {},
journal = {PLoS One},
volume = {7},
number = {7},
pages = {e41244},
abstract = {Disease detection in historical samples currently relies on DNA extraction and amplification, or immunoassays. These techniques only establish pathogen presence rather than active disease. We report the first use of shotgun proteomics to detect the protein expression profile of buccal swabs and cloth samples from two 500-year-old Andean mummies. The profile of one of the mummies is consistent with immune system response to severe pulmonary bacterial infection at the time of death. Presence of a probably pathogenic Mycobacterium sp. in one buccal swab was confirmed by DNA amplification, sequencing, and phylogenetic analyses. Our study provides positive evidence of active pathogenic infection in an ancient sample for the first time. The protocol introduced here is less susceptible to contamination than DNA-based or immunoassay-based studies. In scarce forensic samples, shotgun proteomics narrows the range of pathogens to detect using DNA assays, reducing cost. This analytical technique can be broadly applied for detecting infection in ancient samples to answer questions on the historical ecology of specific pathogens, as well as in medico-legal cases when active pathogenic infection is suspected.}
}
Citation for Study 11376
Citation title:
"Detecting the Immune System Response of a 500 Year-old Inca Mummy".
Study name:
"Detecting the Immune System Response of a 500 Year-old Inca Mummy".
This study is part of submission 11366
(Status: Published).
Citation
Corthals A., Koller A., Martin D., Rieger R., Chen E., Bernaski M., Recagno G., & Davalos L.M. 2012. Detecting the Immune System Response of a 500 Year-old Inca Mummy. PLoS One, 7(7): e41244.
Authors
-
Corthals A.
-
Koller A.
-
Martin D.
-
Rieger R.
-
Chen E.
-
Bernaski M.
-
Recagno G.
-
Davalos L.M.
(submitter)
6314137417
Abstract
Disease detection in historical samples currently relies on DNA extraction and amplification, or immunoassays. These techniques only establish pathogen presence rather than active disease. We report the first use of shotgun proteomics to detect the protein expression profile of buccal swabs and cloth samples from two 500-year-old Andean mummies. The profile of one of the mummies is consistent with immune system response to severe pulmonary bacterial infection at the time of death. Presence of a probably pathogenic Mycobacterium sp. in one buccal swab was confirmed by DNA amplification, sequencing, and phylogenetic analyses. Our study provides positive evidence of active pathogenic infection in an ancient sample for the first time. The protocol introduced here is less susceptible to contamination than DNA-based or immunoassay-based studies. In scarce forensic samples, shotgun proteomics narrows the range of pathogens to detect using DNA assays, reducing cost. This analytical technique can be broadly applied for detecting infection in ancient samples to answer questions on the historical ecology of specific pathogens, as well as in medico-legal cases when active pathogenic infection is suspected.
Keywords
forensic DNA, immune system, proteomics, SH test.
External links
About this resource
- Canonical resource URI:
http://purl.org/phylo/treebase/phylows/study/TB2:S11376
- Other versions:
Nexus
NeXML
- Show BibTeX reference
@ARTICLE{TreeBASE2Ref19603,
author = {Angelique Corthals and Antonius Koller and Dwight Martin and Robert Rieger and Emily Chen and Mario Bernaski and Gabriella Recagno and Liliana M Davalos},
title = {Detecting the Immune System Response of a 500 Year-old Inca Mummy},
year = {2012},
keywords = {forensic DNA, immune system, proteomics, SH test.},
doi = {10.1371/journal.pone.0041244},
url = {http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0041244},
pmid = {},
journal = {PLoS One},
volume = {7},
number = {7},
pages = {e41244},
abstract = {Disease detection in historical samples currently relies on DNA extraction and amplification, or immunoassays. These techniques only establish pathogen presence rather than active disease. We report the first use of shotgun proteomics to detect the protein expression profile of buccal swabs and cloth samples from two 500-year-old Andean mummies. The profile of one of the mummies is consistent with immune system response to severe pulmonary bacterial infection at the time of death. Presence of a probably pathogenic Mycobacterium sp. in one buccal swab was confirmed by DNA amplification, sequencing, and phylogenetic analyses. Our study provides positive evidence of active pathogenic infection in an ancient sample for the first time. The protocol introduced here is less susceptible to contamination than DNA-based or immunoassay-based studies. In scarce forensic samples, shotgun proteomics narrows the range of pathogens to detect using DNA assays, reducing cost. This analytical technique can be broadly applied for detecting infection in ancient samples to answer questions on the historical ecology of specific pathogens, as well as in medico-legal cases when active pathogenic infection is suspected.}
}
- Show RIS reference
TY - JOUR
ID - 19603
AU - Corthals,Angelique
AU - Koller,Antonius
AU - Martin,Dwight
AU - Rieger,Robert
AU - Chen,Emily
AU - Bernaski,Mario
AU - Recagno,Gabriella
AU - Davalos,Liliana M
T1 - Detecting the Immune System Response of a 500 Year-old Inca Mummy
PY - 2012
KW - forensic DNA
KW - immune system
KW - proteomics
KW - SH test.
UR - http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0041244
N2 - Disease detection in historical samples currently relies on DNA extraction and amplification, or immunoassays. These techniques only establish pathogen presence rather than active disease. We report the first use of shotgun proteomics to detect the protein expression profile of buccal swabs and cloth samples from two 500-year-old Andean mummies. The profile of one of the mummies is consistent with immune system response to severe pulmonary bacterial infection at the time of death. Presence of a probably pathogenic Mycobacterium sp. in one buccal swab was confirmed by DNA amplification, sequencing, and phylogenetic analyses. Our study provides positive evidence of active pathogenic infection in an ancient sample for the first time. The protocol introduced here is less susceptible to contamination than DNA-based or immunoassay-based studies. In scarce forensic samples, shotgun proteomics narrows the range of pathogens to detect using DNA assays, reducing cost. This analytical technique can be broadly applied for detecting infection in ancient samples to answer questions on the historical ecology of specific pathogens, as well as in medico-legal cases when active pathogenic infection is suspected.
L3 - 10.1371/journal.pone.0041244
JF - PLoS One
VL - 7
IS - 7
ER -
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