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Citation for Study 11444

Citation title: "Toward a DNA Taxonomy of Alpine Rhithrogena (Ephemeroptera: Heptageniidae) Using a Mixed Yule-Coalescent Analysis of Mitochondrial and Nuclear DNA".

Study name: "Toward a DNA Taxonomy of Alpine Rhithrogena (Ephemeroptera: Heptageniidae) Using a Mixed Yule-Coalescent Analysis of Mitochondrial and Nuclear DNA".

This study is part of submission 11434 (Status: Published).

Citation

Vuataz L., Sartori M., Wagner A., & Monaghan M.T. 2011. Toward a DNA Taxonomy of Alpine Rhithrogena (Ephemeroptera: Heptageniidae) Using a Mixed Yule-Coalescent Analysis of Mitochondrial and Nuclear DNA. PLoS ONE, .

Authors

• Vuataz L. (submitter) +41213163505
• Sartori M.
• Wagner A.
• Monaghan M.T.

Abstract

Aquatic larvae of many Rhithrogena mayflies (Ephemeroptera) inhabit sensitive Alpine environments. A number of species are on the IUCN Red List and many recognized species have restricted distributions and are of conservation interest. Ambiguous morphological differences among closely related species suggest that the current taxonomy may not accurately reflect the evolutionary diversity of the group. Here we examined the species status of ca. 50% of European Rhithrogena diversity using a widespread sampling scheme of Alpine species that included individuals from 22 type localities, analyses of one standard mtDNA marker using the general mixed Yule-coalescent (GMYC) model, and one newly developed nDNA marker, and morphological identification where possible. Using sequences from 533 individuals from 144 sampling localities, we observed significant clustering of the mitochondrial (cox1) marker into 31 GMYC species. Twenty-one of these could be identified based on the presence of topotypes (expertly identified specimens from type locality of the species) or unambiguous morphology. Significant clustering was not detected with protein-coding nuclear PEPCK and incongruence in the two data sets was probably resulted from incomplete sorting of ancestral polymorphism. Nonetheless, nine GMYC species were congruent with nDNA genotype clusters. Bayesian phylogenetic analyses of both gene regions recovered four of the six recognized Rhithrogena species groups in our samples as monophyletic. The study provides a broad overview of the extent of both cryptic diversity and taxonomic oversplitting in Rhithrogena. Future development of more nuclear markers would facilitate multi-locus analysis of unresolved, closely related species pairs. The DNA taxonomy developed here lays the groundwork for a future revision of the important but cryptic Rhithrogena genus in Europe.

Keywords

species delimitation; mtDNA; nDNA; GMYC; DNA taxonomy; Rhithrogena; Alps; species groups; type locality

External links

About this resource

• Canonical resource URI: http://purl.org/phylo/treebase/phylows/study/TB2:S11444
• Other versions: Nexus NeXML
• Show BibTeX reference

  		@ARTICLE{TreeBASE2Ref19658,
  	 author = {Laurent  Vuataz and Michel  Sartori and Andr?  Wagner and Michael T Monaghan},
  	 title = {Toward a DNA Taxonomy of Alpine Rhithrogena (Ephemeroptera: Heptageniidae) Using a Mixed Yule-Coalescent Analysis of Mitochondrial and Nuclear DNA},
  	 year = {2011},
  	 keywords = {species delimitation; mtDNA; nDNA; GMYC; DNA taxonomy; Rhithrogena; Alps; species groups; type locality},
  	 doi = {},
  	 url = {http://},
  	 pmid = {},
  	 journal = {PLoS ONE},
  	 volume = {},
  	 number = {},
  	 pages = {},
  	 abstract = {Aquatic larvae of many Rhithrogena mayflies (Ephemeroptera) inhabit sensitive Alpine environments. A number of species are on the IUCN Red List and many recognized species have restricted distributions and are of conservation interest. Ambiguous morphological differences among closely related species suggest that the current taxonomy may not accurately reflect the evolutionary diversity of the group. Here we examined the species status of ca. 50% of European Rhithrogena diversity using a widespread sampling scheme of Alpine species that included individuals from 22 type localities, analyses of one standard mtDNA marker using the general mixed Yule-coalescent (GMYC) model, and one newly developed nDNA marker, and morphological identification where possible. Using sequences from 533 individuals from 144 sampling localities, we observed significant clustering of the mitochondrial (cox1) marker into 31 GMYC species. Twenty-one of these could be identified based on the presence of topotypes (expertly identified specimens from type locality of the species) or unambiguous morphology. Significant clustering was not detected with protein-coding nuclear PEPCK and incongruence in the two data sets was probably resulted from incomplete sorting of ancestral polymorphism. Nonetheless, nine GMYC species were congruent with nDNA genotype clusters. Bayesian phylogenetic analyses of both gene regions recovered four of the six recognized Rhithrogena species groups in our samples as monophyletic. The study provides a broad overview of the extent of both cryptic diversity and taxonomic oversplitting in Rhithrogena. Future development of more nuclear markers would facilitate multi-locus analysis of unresolved, closely related species pairs. The DNA taxonomy developed here lays the groundwork for a future revision of the important but cryptic Rhithrogena genus in Europe.}
  }
  		

• Show RIS reference

  		TY  - JOUR
  ID  - 19658
  AU  - Vuataz,Laurent 
  AU  - Sartori,Michel 
  AU  - Wagner,Andr? 
  AU  - Monaghan,Michael T
  T1  - Toward a DNA Taxonomy of Alpine Rhithrogena (Ephemeroptera: Heptageniidae) Using a Mixed Yule-Coalescent Analysis of Mitochondrial and Nuclear DNA
  PY  - 2011
  KW  - species delimitation; mtDNA; nDNA; GMYC; DNA taxonomy; Rhithrogena; Alps; species groups; type locality
  UR  - http://dx.doi.org/
  N2  - Aquatic larvae of many Rhithrogena mayflies (Ephemeroptera) inhabit sensitive Alpine environments. A number of species are on the IUCN Red List and many recognized species have restricted distributions and are of conservation interest. Ambiguous morphological differences among closely related species suggest that the current taxonomy may not accurately reflect the evolutionary diversity of the group. Here we examined the species status of ca. 50% of European Rhithrogena diversity using a widespread sampling scheme of Alpine species that included individuals from 22 type localities, analyses of one standard mtDNA marker using the general mixed Yule-coalescent (GMYC) model, and one newly developed nDNA marker, and morphological identification where possible. Using sequences from 533 individuals from 144 sampling localities, we observed significant clustering of the mitochondrial (cox1) marker into 31 GMYC species. Twenty-one of these could be identified based on the presence of topotypes (expertly identified specimens from type locality of the species) or unambiguous morphology. Significant clustering was not detected with protein-coding nuclear PEPCK and incongruence in the two data sets was probably resulted from incomplete sorting of ancestral polymorphism. Nonetheless, nine GMYC species were congruent with nDNA genotype clusters. Bayesian phylogenetic analyses of both gene regions recovered four of the six recognized Rhithrogena species groups in our samples as monophyletic. The study provides a broad overview of the extent of both cryptic diversity and taxonomic oversplitting in Rhithrogena. Future development of more nuclear markers would facilitate multi-locus analysis of unresolved, closely related species pairs. The DNA taxonomy developed here lays the groundwork for a future revision of the important but cryptic Rhithrogena genus in Europe.
  L3  - 
  JF  - PLoS ONE
  VL  - 
  IS  - 
  ER  -