@ARTICLE{TreeBASE2Ref19897,
author = {Patrik Inderbitzin and Richard M Bostock and R Michael Davis and Toshiyuki Usami and W Harold Platt and Krishna V Subbarao},
title = {Phylogenetics and taxonomy of the fungal vascular wilt pathogen Verticillium, with the descriptions of five new species},
year = {2011},
keywords = {Ascomycetes, Pezizomycotina, Hypocreomycetes, Hypocreomycetidae, Plectosphaerellaceae},
doi = {10.1371/journal.pone.0028341},
url = {http://www.plosone.org/article/info:doi/10.1371/journal.pone.0028341},
pmid = {},
journal = {PloS One},
volume = {6},
number = {12},
pages = {e28341},
abstract = {Knowledge of pathogen biology and genetic diversity is a cornerstone of effective disease management. Species names provide an ideal framework for storage and retrieval of relevant information, a system that is contingent on a clear understanding of species boundaries and consistent species identification. Verticillium, a genus of ascomycete fungi, contains important plant pathogens whose species boundaries have been ill defined. Using phylogenetic analyses, morphological investigations and comparisons to herbarium material and the literature, we established a solid taxonomic framework for Verticillium comprising ten species, five of which were new to science. We used a collection of 74 isolates representing as much of the known diversity of Verticillium as possible, and phylogenetic analyses based on the ribosomal internal transcribed spacer region (ITS), partial sequences of the protein coding genes actin (ACT), elongation factor 1-alpha (EF), glyceraldehyde-3-phosphate dehydrogenase (GPD) and tryptophan synthase (TS). Combined analyses of the ACT, EF, GPD and TS datasets using three different algorithms recognized two major groups within Verticillium, Clade Flavexudans and Clade Flavnonexudans, reflecting the respective production and absence of yellow hyphal pigments. Clade Flavexudans comprised V. albo-atrum and V. tricorpus as well as the new species V. zaregamsianum, V. isaacii and V. klebahnii, of which the latter two were morphologically indistinguishable from V. tricorpus but may differ in pathogenicity. Clade Flavnonexudans comprised V. nubilum, V. dahliae and V. longisporum, as well as the two new species V. alfalfae and V. nonalfalfae which resembled the distantly related V. albo-atrum in morphology. Apart from the diploid hybrid V. longisporum, each of the ten species corresponded to a single clade in the phylogenetic tree comprising just one ex-type strain, thereby establishing a direct link to a name tied to a herbarium specimen. A morphology-based key is provided for identification to species or species groups, however, due to instability of many of the diagnostic morphological characters, DNA based identification is required for authoritative identification of Verticillium species.}
}
Citation for Study 11756
Citation title:
"Phylogenetics and taxonomy of the fungal vascular wilt pathogen Verticillium, with the descriptions of five new species".
Study name:
"Phylogenetics and taxonomy of the fungal vascular wilt pathogen Verticillium, with the descriptions of five new species".
This study is part of submission 11746
(Status: Published).
Citation
Inderbitzin P., Bostock R.M., Davis R.M., Usami T., Platt W.H., & Subbarao K.V. 2011. Phylogenetics and taxonomy of the fungal vascular wilt pathogen Verticillium, with the descriptions of five new species. PloS One, 6(12): e28341.
Authors
-
Inderbitzin P.
(submitter)
530 752 8015
-
Bostock R.M.
-
Davis R.M.
-
Usami T.
-
Platt W.H.
-
Subbarao K.V.
Abstract
Knowledge of pathogen biology and genetic diversity is a cornerstone of effective disease management. Species names provide an ideal framework for storage and retrieval of relevant information, a system that is contingent on a clear understanding of species boundaries and consistent species identification. Verticillium, a genus of ascomycete fungi, contains important plant pathogens whose species boundaries have been ill defined. Using phylogenetic analyses, morphological investigations and comparisons to herbarium material and the literature, we established a solid taxonomic framework for Verticillium comprising ten species, five of which were new to science. We used a collection of 74 isolates representing as much of the known diversity of Verticillium as possible, and phylogenetic analyses based on the ribosomal internal transcribed spacer region (ITS), partial sequences of the protein coding genes actin (ACT), elongation factor 1-alpha (EF), glyceraldehyde-3-phosphate dehydrogenase (GPD) and tryptophan synthase (TS). Combined analyses of the ACT, EF, GPD and TS datasets using three different algorithms recognized two major groups within Verticillium, Clade Flavexudans and Clade Flavnonexudans, reflecting the respective production and absence of yellow hyphal pigments. Clade Flavexudans comprised V. albo-atrum and V. tricorpus as well as the new species V. zaregamsianum, V. isaacii and V. klebahnii, of which the latter two were morphologically indistinguishable from V. tricorpus but may differ in pathogenicity. Clade Flavnonexudans comprised V. nubilum, V. dahliae and V. longisporum, as well as the two new species V. alfalfae and V. nonalfalfae which resembled the distantly related V. albo-atrum in morphology. Apart from the diploid hybrid V. longisporum, each of the ten species corresponded to a single clade in the phylogenetic tree comprising just one ex-type strain, thereby establishing a direct link to a name tied to a herbarium specimen. A morphology-based key is provided for identification to species or species groups, however, due to instability of many of the diagnostic morphological characters, DNA based identification is required for authoritative identification of Verticillium species.
Keywords
Ascomycetes, Pezizomycotina, Hypocreomycetes, Hypocreomycetidae, Plectosphaerellaceae
External links
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http://purl.org/phylo/treebase/phylows/study/TB2:S11756
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- Show BibTeX reference
@ARTICLE{TreeBASE2Ref19897,
author = {Patrik Inderbitzin and Richard M Bostock and R Michael Davis and Toshiyuki Usami and W Harold Platt and Krishna V Subbarao},
title = {Phylogenetics and taxonomy of the fungal vascular wilt pathogen Verticillium, with the descriptions of five new species},
year = {2011},
keywords = {Ascomycetes, Pezizomycotina, Hypocreomycetes, Hypocreomycetidae, Plectosphaerellaceae},
doi = {10.1371/journal.pone.0028341},
url = {http://www.plosone.org/article/info:doi/10.1371/journal.pone.0028341},
pmid = {},
journal = {PloS One},
volume = {6},
number = {12},
pages = {e28341},
abstract = {Knowledge of pathogen biology and genetic diversity is a cornerstone of effective disease management. Species names provide an ideal framework for storage and retrieval of relevant information, a system that is contingent on a clear understanding of species boundaries and consistent species identification. Verticillium, a genus of ascomycete fungi, contains important plant pathogens whose species boundaries have been ill defined. Using phylogenetic analyses, morphological investigations and comparisons to herbarium material and the literature, we established a solid taxonomic framework for Verticillium comprising ten species, five of which were new to science. We used a collection of 74 isolates representing as much of the known diversity of Verticillium as possible, and phylogenetic analyses based on the ribosomal internal transcribed spacer region (ITS), partial sequences of the protein coding genes actin (ACT), elongation factor 1-alpha (EF), glyceraldehyde-3-phosphate dehydrogenase (GPD) and tryptophan synthase (TS). Combined analyses of the ACT, EF, GPD and TS datasets using three different algorithms recognized two major groups within Verticillium, Clade Flavexudans and Clade Flavnonexudans, reflecting the respective production and absence of yellow hyphal pigments. Clade Flavexudans comprised V. albo-atrum and V. tricorpus as well as the new species V. zaregamsianum, V. isaacii and V. klebahnii, of which the latter two were morphologically indistinguishable from V. tricorpus but may differ in pathogenicity. Clade Flavnonexudans comprised V. nubilum, V. dahliae and V. longisporum, as well as the two new species V. alfalfae and V. nonalfalfae which resembled the distantly related V. albo-atrum in morphology. Apart from the diploid hybrid V. longisporum, each of the ten species corresponded to a single clade in the phylogenetic tree comprising just one ex-type strain, thereby establishing a direct link to a name tied to a herbarium specimen. A morphology-based key is provided for identification to species or species groups, however, due to instability of many of the diagnostic morphological characters, DNA based identification is required for authoritative identification of Verticillium species.}
}
- Show RIS reference
TY - JOUR
ID - 19897
AU - Inderbitzin,Patrik
AU - Bostock,Richard M
AU - Davis,R Michael
AU - Usami,Toshiyuki
AU - Platt,W Harold
AU - Subbarao,Krishna V
T1 - Phylogenetics and taxonomy of the fungal vascular wilt pathogen Verticillium, with the descriptions of five new species
PY - 2011
KW - Ascomycetes
KW - Pezizomycotina
KW - Hypocreomycetes
KW - Hypocreomycetidae
KW - Plectosphaerellaceae
UR - http://www.plosone.org/article/info:doi/10.1371/journal.pone.0028341
N2 - Knowledge of pathogen biology and genetic diversity is a cornerstone of effective disease management. Species names provide an ideal framework for storage and retrieval of relevant information, a system that is contingent on a clear understanding of species boundaries and consistent species identification. Verticillium, a genus of ascomycete fungi, contains important plant pathogens whose species boundaries have been ill defined. Using phylogenetic analyses, morphological investigations and comparisons to herbarium material and the literature, we established a solid taxonomic framework for Verticillium comprising ten species, five of which were new to science. We used a collection of 74 isolates representing as much of the known diversity of Verticillium as possible, and phylogenetic analyses based on the ribosomal internal transcribed spacer region (ITS), partial sequences of the protein coding genes actin (ACT), elongation factor 1-alpha (EF), glyceraldehyde-3-phosphate dehydrogenase (GPD) and tryptophan synthase (TS). Combined analyses of the ACT, EF, GPD and TS datasets using three different algorithms recognized two major groups within Verticillium, Clade Flavexudans and Clade Flavnonexudans, reflecting the respective production and absence of yellow hyphal pigments. Clade Flavexudans comprised V. albo-atrum and V. tricorpus as well as the new species V. zaregamsianum, V. isaacii and V. klebahnii, of which the latter two were morphologically indistinguishable from V. tricorpus but may differ in pathogenicity. Clade Flavnonexudans comprised V. nubilum, V. dahliae and V. longisporum, as well as the two new species V. alfalfae and V. nonalfalfae which resembled the distantly related V. albo-atrum in morphology. Apart from the diploid hybrid V. longisporum, each of the ten species corresponded to a single clade in the phylogenetic tree comprising just one ex-type strain, thereby establishing a direct link to a name tied to a herbarium specimen. A morphology-based key is provided for identification to species or species groups, however, due to instability of many of the diagnostic morphological characters, DNA based identification is required for authoritative identification of Verticillium species.
L3 - 10.1371/journal.pone.0028341
JF - PloS One
VL - 6
IS - 12
ER -
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